Is it possible to compare data between real-time PCR plates - (Nov/16/2010 )
My question is regarding whether it is possible to compare expression of genes between realtime PCR plates if I include the same calibrator sample in each plate which I use for the deltadelta Ct calculation each time. In my experiment I have samples from 5 different patients but due to the number of treatments I can only fit one patient per plate. So I just wanted to double check that it is possible to compare data collected from different Qrt-PCR runs.
Thanks in advance for your help.
Yes, it is possible. If you are using the ABI program, go to New Analysis -> Add Plates.