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Is it possible to compare data between real-time PCR plates - (Nov/16/2010 )


My question is regarding whether it is possible to compare expression of genes between realtime PCR plates if I include the same calibrator sample in each plate which I use for the deltadelta Ct calculation each time. In my experiment I have samples from 5 different patients but due to the number of treatments I can only fit one patient per plate. So I just wanted to double check that it is possible to compare data collected from different Qrt-PCR runs.

Thanks in advance for your help.


Yes, it is possible. If you are using the ABI program, go to New Analysis -> Add Plates.