sequencing problem after pcr - (Mar/16/2009 )
HI, i would like to ask about is it feasible to mix 2 tubes of pcr product (different mgcl2 concentration) for purification from gel and then for sequencing?
the products have the same size (same position).
Its okay, no problem.
same gene and organism????
Yeah........Gebirgsziege has a point.....its got to be teh same product from the same gene and organism.........from yr post I took it for granted that the only difference between the 2 tubes if magnesium ion concentration.
gebirgsziege on Mar 16 2009, 06:59 PM said:
THX for all the replies, ya, it is from the same gene and same organism. only the magnesium chloride concentrations are different. really thz alot!