designing primers - (Jul/23/2009 )
Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words is the part of amplifing.
I am looking forward to your answers!
-Christina-
Christina on Jul 23 2009, 02:58 PM said:
Could anyone give me some advice about how to design good primers for the target sequence. I plan to carry bisulfite sequencing and need good primers. I design many pairs of primers but I could not get any good result. it seems as a difficult task.I'm worried about my job,I wish somebody could help me solving my problem,thanks. here my sequence,anybody can help me designing any primers?yellow words is the part of amplifing.
I am looking forward to your answers!
you can use methyla primer express, can just paste your target sequence and get the primers for BSP or MSP
-RKA-