Faint band and primer dimer in PCR - (Nov/16/2017 )
Hi everybody, can you explain my situation ? When i run electrophoresis after pcr, in 3 wells , only 2 of 3 present desired band and all 3 wells have primer-dimer. The primer- dimer is brighter than my desired band. Help me please !!!!!
-Bao Duong-
trite primer concentration or rise annealing temperature.
-merlav-