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probe conc. of Asymmetric PCR - (Aug/01/2009 )

Does anyone know what concentration I should use for Asymmetirc PCR? Since the probe can't prime to the target (no extra melting peak apper).

Currently, I use
Reverse Primer 500nM
forward primer 100nM
probe 500nM

Thank you


The unlabelled probe is

What is the possible factor that make it can't bind to the sequence?