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PCR troubleshoooooting - primer dimer (Jan/13/2010 )

Hi all,,Iam amplifying specific gen by PCR, I got the product last time ,,but know i got two band , i think of the primer dimer,,what is the solution? is it contamination issue? :blink:

-maxmix-

maxmix on Jan 14 2010, 11:49 AM said:

Hi all,,Iam amplifying specific gen by PCR, I got the product last time ,,but know i got two band , i think of the primer dimer,,what is the solution? is it contamination issue? :blink:



Hey, it would be better if you also put the gel pic. need more details. What is the size of the other band?? maybe, last time u did the PCR, the extra band was there, running close to ur desired product and u didnt resolve it well enough (and therefore it appeared as a single band)....

-DRN-