PCR double-bands - (Feb/11/2011 )
Im running some PCRs with bacterial DNA as sample and primers that are "universal" for 16S.
The sample I have should have a fairly low number of bacteria in.
I thought I would just ask the people in here if you have any explanations to these "double-bands" that I get.
Does it indicate several species of bacteria or could it be something else?
Different allels perhaps? Though I'm not sure if bacteria have this...Or different strains or clones that have some difference in this DNA region? Depends what samples you used.