Primer design - Primer design (Jan/03/2011 )

Why when we make Forward primer we keep the sequence as it is without doing it in complementary, while we do reverse complement in the reverse primers. any clue

science-lover on Tue Jan 4 01:59:30 2011 said:

The DNA-polymerase can only sinthesize in  5' to 3' direction. Your fwd primer is in on the beginning  of the + strand in 5' to 3' direction, and your reverse primer is on the beginnig of the -strand in 5' to 3' (or if it easier to visualize for you: on end of the - strand in 3' to 5' direction). So after you amplify your desired  gene with this primers you will get the dsDNA with fwd and reverse primer (http://lslab.lscore.ucla.edu/Mitochondria/primer.htm).
Also, there are lots of  other useful sites about primer design, just google it. Here are some I came across: PCR_Primer Design, primer_design


Cheers 

Thanks Lab_microbe. this was very helpful.