Resuspending primers calculation - (Jul/11/2013 )
I'm having problems trying to understand de chemestry in resuspending primers. I know I can use online calculators, and I have the general formula like multiply the initial (dry) concentration x10 and get a final concentration of 100 ul/L or
Usually primers (oligos) come in lyphilized powder with the amount (mass) indicated on the tube, for example, 4.9 nmol. We usually reconstitute the primer to make a 100 uM/L stock solution, we will need to add 49 ul TE buffer to the tube (4.9 nM/49 ul = 100 um/L). So if you know the amount of the primer from the tube, it is easy to determine how much solvent is needed without any calculation.
To put it in a simple way, if you have
be careful with nomenclature. uM=umol/L. uM/L is, at best, redundant, at worst, meaningless.
Thank you very much for your answers. My confusion is with nomenclature indeed.
We multiply nmol of primers by 50 to find the micro liter of water that should be added to them.
primer GAPDH-F nMol is 20
so multiply it by 50=> 20*50= 1000
now add 1000 microlitter of clean water to it and mix.
now 1micro litter is enough for 10 to 50 micro litter of pcr reaction.