RT-PCR Gel - Ladder looks terrible and product is fuzzy! (Apr/21/2010 )
I'm having some issues running a gel. My product is ~80 bp so I am running it on a 2% gel at 90-100 V for 40 minutes. The ladder isn't getting good separation and my product appears fuzzy. Does anyone have any suggestions? Thanks!
ｍｙ products is 206bpｓ，3% gel.The ladder got good separation. you ｍａｙ ｔｒｙ ｔｏ increase your gel ｃｏｎｃｅｎｔｒａｔｉｏｎ．ｔｒｙ ３％ ｇｅｌ ｏｒ ｈｉｇｈｅｒ．