Wrong PCR product - (Oct/11/2013 )
I have some problem with my PCR product. I design primer base on invitrogen. The expected size of my PCR product is 290bp. I do with RNA (RT-PCR one step). But The band is very nice but the size about 400bp. Please give me some advice? Thank you
-vpvatuan-
Your primer might recognise another gene than the one you want to detect, giving a completely different fragment. Try with a different pair of primers. An alternative might be designing primers with PrimerBLAST or looking for ones that have already been used in publications.
-Tabaluga-
Thank you Tabaluga, I try to do primerblast but it not similar with another gene.
-vpvatuan-