Is Taq polymerase still active after staying at 10C for one day? - (Oct/24/2009 )
I accidentally left the polymerase tube outside the fridge for one day, (temp is around 10C since it previously in the ice bath...). Can I still use this Taq polymerase? Thanks!
DigitalPig on Oct 24 2009, 06:49 PM said:
Yes. Give it a try. It should be okay from this miss adventure.
Yes, almost certainly. Taq is a high temperature enzyme which survives 95C in a thermocycler. The main danger would be bacterial or fungal growth, but this is unlikely over a day, and probably would be inhibited by other components of the enzyme buffer.