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PCR problem - (Sep/05/2013 )



When I use above 15 DNA samples, my PCR doesn' t work but when I use below 15 (same DNA), the pcr is perfect. I use invitrogen' s taq dna polymerase, dntp. According to you what is the problem?


some more details would be good. Are you using a mastermix?


Yes, I use mm everytime.