Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

Primer design for qPCR - (Jul/09/2010 )

Hi all,
could anyone tell me how to design primers for qPCR?
I'm not very good in bioinformatics... B)


Designing qPCR primers should be as easy as using the software that comes with your qPCR instrument. If you do not have access to such software, you can use software available online, like IDT's:

There are also online tutorials on what you need to consider, like this one from Premier Biosoft:

Good luck