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difference between PCR primers and sequencing primers - (Jan/15/2011 )

Hi
Is there any differences between primer design rules for PCr and sequencing reactions?
actually I design some primers for sequencing according to my experience on pcr primer design but it didnt work, so is there any special issue in designing sequencing primers?

regards

-sara.r-

You can often use the primers you used for PCR for sequencing reactions, however some will work much better than others (don't really know why). I would try and when it doesn't work, I'd redesign internal primers.
What is the annealing temperature of your PCR?

-Maddie-