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PCR using genomic DNA - (Oct/15/2009 )

Anyone has some experiences on the pcr using transformed yeast genomic DNA as template. i think the transformation worked, but sometimes i can not get the band by PCR. some ideas?


We need you protocol before we can help at all...


i think swanny s right.. we have no information watsoever!!!
and u think it has worked?? why do you think so??
please provide some basic information for us to be able to troubleshoot!!!

-Pradeep Iyer-