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PCR reagents, can I use reagents from different manufacturers? - (Mar/31/2010 )

Hi all,

I am mainly doing clinical screening using nested PCR in my lab.
And I am wondering whether would it be OK to use reagents from different manufacturers,
ie using Taq from Invitrogen and dNTP from Fermentas.
Would doing so have any effect on my results?

Any input is greatly appreciated!
Thanks!

-Square Pig-

It should work fine. I'm not sure about how it would affect GLP issues in a clinical lab, where I would expect there to be well established, somewhat rigid protocols.

-phage434-

dNTP s from any relaible source dosnt effect results but efficiency of taq or any other enzyme shuld be optimized and confirmed before starting any serious and sensitive type of reaction.

as i dnt run clinical type of reaction bt once i face different band intensities from different sources of taq.

-chicken-

Hi all,

Thank you for the input.
Attached File

Attached File

-Square Pig-

Hey square pig,
Did it end up working okay?

I have been looking for the same answer about using different manufacturers. I am thinking about using these reagents from YSI.

Any help would be appreciated. Thanks

-Eric

-reagent tester-