Using qPCR machine for simple PCR, is it possible? - (Sep/29/2015 )
Hi everyone! We've got only a qPCR machine in our lab, but I need to do a simple PCR. Maybe it is a stupid question, but I haven't got a clean answer yet.
-p.blaze53-
Yes, you can simply ignore the fluorescence readings. But they are useful in monitoring a normal PCR, since you can tell how many cycles are really required to amplify your sample.
-phage434-
Thank you for your useful answer!
-p.blaze53-
You can also take the products of a qPCR (or regular PCR run on a qPCR machine) and run them on a gel like normal.
-bob1-