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problems with Bisulfite PCR - (Feb/12/2012 )


I'm new to the the world of methylation and am trying to amplify CpG islands after bisulfite conversion. But when i run the PCR products on a gel i get smears and they aren't even in the correct range. I designed my primers using meth primer and when the first PCR didn't work i tried lowering the extension temperature to 63 C ( first i used 72 C).

Any suggestions would be greatly appreciated.


most liekly a primer design problem, I would try to redesign them again, maybe using MethylPrimer Express which is freely available.