polymerase to use for cloning - (Mar/06/2013 )

Lets say I wanted to clone a region of DNA (a 2.0 kB putative promoter region) from genomic DNA into a reporter vector, like pGL3 basic, by PCR-mediated amplification.

Should I use a high fidelity polymerase for the PCR or just regular Taq?

If I use a high fidelity polymerase, which one should I use?

always better to go with high fidelity pol for cloning. there are lot available in the market, like Phusion (Neb), platinum pfx , Accuprime Pfx (invitrogen), Pfuultra (stratagene) etc ....i would go with Accuprime, as i am personally used to that.

I like KOD, but the pfu range tends to be the standard.

bob1 on Wed Mar 6 19:47:30 2013 said:

What is KOD? What do you mean by "pfu range?" Thanks.

KOD is KOD polymerase

pfu range means any one of the DNA pols that are derived from pfu (e.g. pfuUltra).