Universal 16s rRNA primers needed - (Oct/30/2009 )
I need your help.
I need a universal 16s rRNA primers which is already made as commercials.
Do you know which company have this pre-made Universal 16s rRNA primers? (this is not custom made)
Why don't you just pull them out of a publication?
Here's a link. http://aem.asm.org/cgi/content/abstract/AEM.02220-08v1
See this page:
try following primers known as 27F and 1492R, they amplify a long 1.5 kb almost all of 16S gene, they work perfectly. do tell if u need short segments
27f AGA GTT TGA TCC TGG CTC AG
1492 TAC GGT TAC CTT GTT ACG ACT T