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pcr - (May/12/2015 )

Hi all, I got well amplified non specifically annealed bands in my GM testing PCR at 58.4 annealing temperature, even at 0.2 microliter volume of primers in a 15 microliter reaction volume. I also got  the gm positive band along with that. what can I do to prevent it?

Thanks 

-Chomolungma-

Contamination? Try different temperatures/conditions?

-bob1-