Primer Dilution Problem - D'oh! (Sep/22/2010 )
Hello one and all. I have a problem and I was hoping you all could give me some feedback!
I received in some oligonucleotide primers today (joy!) and diluted them down to my lab's required concentration (250 pmol/uL). Anyway, I diluted the first two primers (corresponding to one PCR rxn) and realized I was using DEPC-treated water, and not buffer TE! Gah! Should I just bite the bullet and dilute more to a lower concentration in TE and water? Or will the DNA be fine in water? We store our oligos in a -20 freezer. Your help is GREATLY appreciated!
Your oligos will be just fine in water at -20oC. The worst that can happen is that they will not last as long, but easily months in water when frozen.
Thank you so much! I was freaking out! I'm just a lowly Master's Student in my lab and my PhD student is, hmm, particular about how we do things. Haha! Again, thank you for your help!