a doubt about PCR gel purification - (Mar/25/2009 )
I want ask you a very simple question. I´m going to use the Qiagen gel extraction kit to purify a PCR amplified product from an agarose gel with thidium bromide, and I want ask you: how do you avoid ethidium bromide contamination using this kit? Do you do "something" betwen cutting the gel and putting it into the tube?
Just wear gloves.
thanks, but I mean how take ethidium bromide out from DNa, is it possible?
survi on Mar 25 2009, 03:43 PM said:
As far as I know the kit does that for you. Between gel and kit you dont need to do anything, and then your DNA is ready for any downstream use you need.
Dont worry about Ethidium bromide it will go off during the washing step of PCR product purification. EtBr never form any strong bounding with DNA so simple washing is enough to remove EtBr from PCR...so there will no Etbr in your pcr product...