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How to calculate the price of PCR test per sample? - (Dec/04/2011 )

I need to calculate the price of PCR test per sample ,how can I do it?
What items must be calculated other than kit price and solutions
Thanks in advance

Thanks all
Now I need to know how much does one sample cost in a biotechnology center?


working time, fail-rate?


ongoing maintenance of your PCR machine? (although per sample that is probably negligible?.....)


cost of extracting DNA (if you are doing this for PCR only)


How much time has a technician spent on standardising the reaction must also be accounted for.


Also pipette use and tips.


Agarose and Gel running related stuffs??


plastic ware, tips - major part of the costs!


if using etbr - the disposal costs for contaminated material