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No PCR amplification with b-actin primers - (Jun/22/2012 )


I'm trying to amplify the b-actin gene in Oncorhynchus mykiss in conventional PCR. I designed the primers with bioinformatic programs but nothing is working, I haven't found primers for DNA for this specie, or similar, like salmo salar... Does anyone have work with this procedure? I need more ideas for the primer design

please, help!


It might be a problem in your PCR master mix or any component of that.

The first thing is, did you get anything in your PCR. I used to design my primers using primer design(applied bio system) and it works for me.