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Touchdown PCR - (Apr/27/2009 )

Hey all,

Just wondering if anyone has a pretty generic touchdown pcr protocol? doesnt have to be too specific :)


I usually use the following: 2x 65 C, 2 x 62C, 2 x 60C, 5 x 58 C, 20 x 56 C

works fine for me :wacko: