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DNA polymerase for GC rich template? - (Jan/08/2013 )

In among all the brands of DNA polymerase, which one stand out among all?
I found out Bioline's MyTaq look pretty good when compare to other vendors' DNA polymerase with DNA template of 61%GC.
Anyone uses Bioline's product before?

-badguy-

you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

-Curtis-

I guess it depends on the target DNA in the end and you have to try out...there is no best one.

-hobglobin-

Curtis on Wed Jan 9 15:27:05 2013 said:


you can't really say. I'm doing some difficult pcr with Finnzyme's Phusion which comes with a GC buffer only for GC rich sequeces. We used Bioline's, it didn't amplify our fragment

What Bioline's polymerase you used?

-badguy-

BIO-X-ACT™ Long DNA Polymerase

-Curtis-