Measuring pcr fragments in a gel - (Jun/27/2014 )
I have two fragments (~ 1kb) , one is 7 bp larger than the other, can I run them in a denser (higher %) agarose gel to figure out which is which? What type of concentration should i use?
Short answer: No.
You will never tell the difference between those fragments on an agarose gel.
Can you cut one of them into shorter fragments with an enzyme?