PCR -No band formation - (Aug/04/2010 )
I used the PV92 Informatics Kit from BIO-RAD to amplify the samples in the PCR. I followed the protocol as mentioned in the manual. After i ran the samples in the agarose gel, I could see only bands from the Molecular Mass Ladder(MMR), and migration of loading dye from the other wells. Is there any way I can check till what stage the protocol works and any idea as to why it is not working?
I have attached a picture of the gel after it was stained. The right most well was loaded with MMR (the one that has bands)
Thanks in advance for your help !!!
See my other post in the mol biol sub-forum.... you need to start by saying whay you think might have gone wrong...