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Primers mix - (Dec/03/2013 )

I am new to the PCR multiplex stuff and would like to know if its better to make a primer mix or work with any primer separately? I am working with genotyping (SNPs) of multi allelic genes?


-Paulo S Schlögl-

Primer separately.

-Kasturi Borah-

Agree. Keeping the primers separate allow you to use them individually for sequencing, or as primers for a different PCR with a distinct second primer. You can prepare a dilute 10 uM mix, but keep the 100 uM stock primer separate.