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Real Time PCR Primers and Probes - (Dec/14/2010 )

This is the first time for me to design real time PCR primers and probes, Iam seeking for advice which software should I use


It depends on the qPCR instrument you have. If you have one of Applied Biosystem's (Life Tech) instruments they come with their own assay design software. I would recommend using that if you have access to it.



No I have Biorad iCycler