Real Time PCR Primers and Probes - (Dec/14/2010 )

Hi
This is the first time for me to design real time PCR primers and probes, Iam seeking for advice which software should I use

It depends on the qPCR instrument you have. If you have one of Applied Biosystem's (Life Tech) instruments they come with their own assay design software. I would recommend using that if you have access to it.

Cheers

No I have Biorad iCycler