RNA - extraction and pcr (Jul/18/2011 )
Can someone explain to me about my result???
After extract my RNA and ran the gel electrophoresis, I couldnt see any bands for my RNA extraction. However, after synthesized my cDNA (using the same RNA) and did PCR reaction adn ran again the electrophoresis, I could see clear bands with the right size for my housekeeping gene (ubiquitin). I wonder why I cannot see the band at first?
What conditions did you used for RNA gel? Total RNA is usually run in denaturing gel or buffer, stained with EtBr, then you can see two (three) bands of rRNA (tRNA) and smear around which is mRNA. Was that the first time you run RNA on gel or did it work well before?