Protocol Online logo
Top : New Forum Archives (2009-): : PCR, RT-PCR and Real-Time PCR

Gene expression from whole pancreas or islets by using RT-PCR - (Feb/21/2013 )

Dear All,
Sorry, is me again.
I would like to ask for suggestion if it is better to extract the RNA from whole pancreas or from islets of a mice to do the RT-PCR for gene expression study?
Thank you very much.


It depends on the purpose of your study. If you want to look for expression specifically in islets and you can isolate islets, why not just using islets for RNA isolation?


Hi Epigenetist,
Yeah, indeed is better to use islets..
But it is kinda troublesome to isolate RNA from islets than pancreas. I am wondering will there be great difference of the gene expression using islets or pancreas?
Thank you.