how to make a working solution of a primers for pcr reaction?? - (Feb/06/2013 )
How do you know the conc. of lyophilized primer is 20pmol? Is it mentioned in the document that you attached? To make a stock of desired conc. you need to dissolve the lyophilized primer in autoclaved water. And from that stock you take the required amount to make up the final conc. of primer in PCR reaction. check the link for calculator-
The data sheet you attached says: "These Primers and Probe are synthesised and purified by PAGE. They are supplied as 0.2 OD (6.6 μgs) lyophilized. To reconstitute, dissolve in 20 μl sterile water, check concentraion by OD before use."
So, according to this you just have to add 20µl of water. The predicted concentration will be 330ng/µl (6.6µg/20µl), but you should confirm by nan
That is a really strange stock primer concentration. What company are you getting your primers from? Did you design them or are they predesigned? I tried searching for "GeneI" (the name at the top of your .pdf), but nothing came up.
it's this apparently (GeNei Bangalore)...