Information about the use of DNA diluted in Real Time PCR - (Sep/19/2013 )
For the removal os PCR inhibitors for Real Time Detection we should dilute the DNA sample 1:10 fold, consequently we are reducing the amount of DNA available for amplification, this fact cannot give false- negative results?
So for PCR inhibitors removal, what do you recomend to use in daily diagnosis to prevent false negative results?
There isn't anything that I am aware of. The false negatives won't be entirely removed by diluting 1:10, you could also try 1:100, 1:1000 etc.
But the dilutions that you recommend wont increase the chances of the sample give a false negative result? Mainly in the cases of very minimum loads of DNA/ RNA target?
Potentially, though if you are doing qPCR with good primers, then the reaction should be close to 100% efficient, so detection should work from very low abundance targets.
Ok, thank you very much