|
|
Top :
Forum Archives: :
Protein Related Discussions
-
Unstable protein - (reply: 1)
-
Seperating High MW proteins - (reply: 2)
-
Disappearing protein MWM marker - (reply: 1)
-
Purification of GST FUSION PROTEIN - (reply: 1)
-
Protein isolation by Trizol - (reply: 1)
-
Can i send protein band from SDS-PAGE for MAlDI......... - What could be the complication!!!!!!!!!! (reply: 5)
-
protein in inclusion bodies-plz help - (reply: 4)
-
Protein Loading and Western Blot - Western Blot (reply: 5)
-
single stranded binding protein? - how to put into my PCR reaction? (reply: 6)
-
problem in protein expression after 8 months - (reply: 2)
-
mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
-
Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (reply: 1)
-
Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
-
GST protein purification - (reply: 1)
-
Removing all cell surface proteins/receptors - (reply: 3)
-
Pioneers of Mutagenesis to Probe Protein Structure and Function - (reply: 1)
-
heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
-
western blot: why are proteins not separated? - (reply: 4)
-
protein concentration - (reply: 4)
-
troubles with small proteins (less than 20) on SDS page - (reply: 5)
-
protein spreading in gel - (reply: 1)
-
no expression of my target protein - (reply: 3)
-
2D gelelectrophoresis: prestaining of proteins - (reply: 4)
-
general protein purification from mouse tissue - (reply: 4)
-
Different intensity present in reference protein even though BCA performed - (reply: 1)
-
Technique to estimate the level of protein expression from media - (reply: 2)
-
unexpected 40kDa band -E.coli protein expression - (reply: 3)
-
Protein purification by two stage chromatography - MDH by anion exhange & gel filtration (reply: 1)
-
Protein preicipitated after Dialysis - (reply: 7)
-
Methods of concentrating Protein lysate - (reply: 2)
-
DNA motif database / no TF - database of DNA motifs for binding of DNA replication proteins (reply: 2)
-
1% boiling buffer for protein extractions - (reply: 3)
-
Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
-
Gene complexity and protein complexity analysis - (reply: 2)
-
protein extraction from mouse brain tissue - (reply: 1)
-
pROTEIN DOMAINS - new to proteomics (reply: 3)
-
Protein identification - (reply: 1)
-
Phosphatase-Protease inhibitors in TCA total protein extraction - (reply: 2)
-
protein on membrane - (reply: 1)
-
protein concentration problem - (reply: 2)
-
protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
-
Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
-
temperature can effect the protein active? - (reply: 1)
-
storing dialysis tube(bag) - (reply: 2)
-
HIS TAG Protein Estimation - (reply: 5)
-
precipitation of protein with acetone - (reply: 3)
-
Protein Conc using Bradford Assay - (reply: 1)
-
optimal timepoint to collect secreted protein - (reply: 1)
-
Determining if a protein is a downstream target of another protein - (reply: 4)
-
Fast Protein Liquid Chromatography (FPLC)/ HPLC - (reply: 4)
-
protein solubility - (reply: 2)
-
A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
-
Advantages of Proteomics Study - (reply: 1)
-
Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
-
total protein extraction from brain tissue - (reply: 3)
-
smeared proteins western blot - (reply: 5)
-
Protein come from nowhere - we cannot find RNA (reply: 5)
-
Do we have fluorescence genes? - genes which code for fluoscent proteins (reply: 12)
-
what cell line to use for visualizing membrane localized protein - (reply: 1)
-
DNA "snot" in cell lysis/protein extraction - (reply: 6)
-
Protein insolubility problem after bacterial induction - (reply: 2)
-
TAT protein - (reply: 1)
-
Comparing surface protein expression of different cell lines - (reply: 1)
-
Protein elution problems - (reply: 2)
-
Comparative Genomics, Protein Sequencing, more - (reply: 1)
-
finding a protein's function - (reply: 1)
-
Negative Control Rabbit IgG for ChIP Assay - (reply: 3)
-
DNA Protein pull down protocol for mass spec - (reply: 2)
-
Protein ladder - (reply: 8)
-
amount of protein to load for a western? - (reply: 2)
-
protein absorbance wavelength? - (reply: 1)
-
Protein size prediction - (reply: 4)
-
Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
-
Coating Protein A and G on ELISA plate - (reply: 2)
-
Huge mRNA overexpression, but no protein overexpression. - (reply: 10)
-
about transfer low molecular weight protein in tricine PAGE - (reply: 5)
-
protein isocratic HPLC.. preparatin of tris buffer + n-propanol mix - getting variations in Retention time.. (reply: 1)
-
19kda-15kda-6kda.. resolution of these proteins? - which gradient sds-page would be best suitable? thanks! (reply: 2)
-
Protein expression problems in HB101 - (reply: 1)
-
protein expression problem - (reply: 1)
-
protein extraction protocol? - (reply: 3)
-
Identifying a protein isoform - (reply: 1)
-
Trying to detect correct membrane protein size in gel - At wits end (reply: 4)
-
transfer problem - i cannot transfer proteins to a pvdf membrane (reply: 7)
-
isotope labeling of proteins - (reply: 4)
-
TRIZOL for protein extraction - any problem? (reply: 4)
-
Elution problem in Protein G column - Guide me (reply: 2)
-
proteins expression - (reply: 1)
-
Experience with protein G dynabeads? - (reply: 2)
-
Protein Informative Tools - (reply: 1)
-
URGENT: software tools for proteomics - (reply: 1)
-
Why Proteins? - Can't figure proteins out, let's find some help? (reply: 4)
-
DMSO for protein stability at -80oC - protein storage (reply: 4)
-
How to separate very close protein bands - (reply: 5)
-
size exclusion.. how to reduce tailing. for proteins.. - sillica matrix tsk columns.. (reply: 2)
-
oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
-
getting part of a protein sequence - finding protein domain sequences (reply: 1)
-
purification of non his-tagged proteins - (reply: 4)
-
Sample buffer and protein assay - (reply: 1)
-
protein extraction fromn soil bacterium - (reply: 1)
-
how to find the modification enzyme of a target protein - (reply: 1)
-
Residues don't match... why? - in human and mouse proteins (reply: 2)
-
How many ways are there to conc. protein? - (reply: 5)
-
6XHistaq proteins - (reply: 2)
-
minimum protein conc for immunoprecipitation - what's the minimum needed for results (reply: 1)
-
Seperation of unconjugated protein after hapten conjugation - (reply: 1)
-
Protein purification - (reply: 5)
-
Protein Extraction from SDS-PAGE - (reply: 1)
-
probing one protein with multiple antibodies - (reply: 2)
-
Acid hydrolysis of protein - (reply: 1)
-
Subcellular localization of -HA tagged protein - (reply: 1)
-
DNA binding proteins ELISA - need suggestions (reply: 1)
-
Western for a 500kda protein - some troubles, need help! - (reply: 1)
-
Inclusion bodies protein determination - (reply: 1)
-
How to make my protein Soluble? - Bacterial system it sucksss ? (reply: 1)
-
Protein homology modelling - (reply: 3)
-
wrong protein size - (reply: 6)
-
Mysterious protein degradation - (reply: 4)
-
protein extraction from yeast--all extracted proteins are below 50KD - (reply: 1)
-
Acid in Dialysis Tubing - probably a stupid question (reply: 2)
-
Which control protein for Western Blotting of nuclear protein? - (reply: 9)
-
Protein sequence alignment - (reply: 4)
-
Protein isolation from RNAlater stored tissue - (reply: 5)
-
How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
-
Point Mutation, Protein Folding, Base Pairing - (reply: 3)
-
Protein extraction from periplasmic space - a simple protocol anyone? (reply: 1)
-
membrane protein extraction - membrane protein extraction (reply: 7)
-
membrane protein and boiling - (reply: 3)
-
protein band is shifted after boiling sample with sample buffer - (reply: 7)
-
Dialysis, how long? - (reply: 8)
-
Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
-
Removing histone proteins from nuclear protein isolation - (reply: 2)
-
Bradford Assay - Calculation protein concentration (reply: 9)
-
Which quantity of purified protein in WB? - (reply: 1)
-
Protein questions - (reply: 6)
-
Elisa- need help - Elisa - Phosphorylation studies (reply: 1)
-
Dialysis Buffer for Recombinant Protein - (reply: 5)
-
protein recovery problems in analytical size exclusion chromatogrphay - or how do i have a more sensitive method? (reply: 3)
-
to explain why i see two forms the same protein in SDS-PAGE - (reply: 1)
-
Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
-
Frozen Protein A agarose beads - (reply: 1)
-
protein inclusion bodies.. purpose of multiple water washes after lysate centrif - (reply: 5)
-
protein degredation - (reply: 3)
-
protein purification from yeast - (reply: 2)
-
Problem in concentrating protein - (reply: 1)
-
Reverse phase chromatography - protein sample preparation and loading - (reply: 1)
-
protein absorption at 260nm - (reply: 7)
-
can't view the protein band..help! - (reply: 1)
-
unstable protein expression,pleeeeeese help - (reply: 6)
-
cleaving protein - (reply: 8)
-
protein estimation colorimetry.. preferred range.. - statistically more precise & accurate? (reply: 2)
-
Recombinant protein stability - stability issue (reply: 1)
-
Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
-
How to find miRNAs for one designated protein? - miRNA (reply: 1)
-
transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
-
protein normalizer (equal protein loading) - (reply: 3)
-
ddephosphorylating proteins using acid phosphatase - (reply: 1)
-
Fishin out possible interacting protein for known RNA..?! - (reply: 1)
-
Protein extraction supernatant turns black/sooty color - (reply: 4)
-
Protocol for IgG Purification from Ascites? - (reply: 1)
-
experimental determination of protein function - (reply: 2)
-
How to separate proteins of equal size - (reply: 3)
-
Using urea in ion exchange chromatography of proteins - (reply: 1)
-
upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
-
protein tag for ICC/IF - (reply: 2)
-
cytosolic protein collection - (reply: 3)
-
Is there a way to screw up dialysis? - (reply: 8)
-
protein extraction from Daphnia/rotifera - (reply: 3)
-
immobilized protein affecting activity - (reply: 4)
-
Oligomeric protein - how to measure concentration? - (reply: 2)
-
his-tag protein pure but degradated during growth - his-tag protein pure but degradated during growth (reply: 15)
-
precipitation of guanidine HCl-sample after adding 4X laemmli buffer - SDS-PAGE protein sample preparation (reply: 2)
-
Protein expression changes after freezing+thawing - (reply: 1)
-
to cross-link a protein to a polysaccharide, which to choose? Sulfo-NHS or NHS? - (reply: 5)
-
programmes for protein sequences characterization - (reply: 1)
-
band size with flag tagged proteins? - (reply: 3)
-
identification of secretory protein - (reply: 1)
-
Immunization with protein band from SDS-PAGE - (reply: 5)
-
protein extraction - cytosolic lysis buffer (reply: 8)
-
protein elution - any idea to elute protein (reply: 5)
-
caspase-3 cleavage assay - how to test the clevage of a protein by caspase in vitro (reply: 2)
-
what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
-
protein concentration - (reply: 1)
-
bands sizes with sectreted protein - (reply: 1)
-
smearing of proteins during transfer - (reply: 10)
-
contamination of DNA in protein sample - (reply: 1)
-
analysing protein sequences - How to find domains? (reply: 5)
-
Gentle extraction of nuclear protein complex - (reply: 2)
-
Protein into the membrane - (reply: 2)
-
do i need to desalt my protein after purification - (reply: 1)
-
DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
-
reverse phase hplc of proteins.. mechanism of binding and elution.. - your views.. ! (reply: 3)
-
BSA vs. Nonfat dry milk for serine/threonine phosphorylation sites - which is better for quality blots? (reply: 1)
-
Western Blot for weakly expressed protein - (reply: 3)
-
My protein shows 3 bands - Reb1 phosphorylation? (reply: 1)
-
A ROS producer that doesn't cause protein carbonylation - (reply: 2)
-
Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
-
large quantity of high concentration protein needed - (reply: 3)
-
resolving 27 kDa protein - (reply: 3)
-
small volume spectrophotometrical protein quantification - (reply: 1)
-
HELP: protocol for protein extraction - (reply: 4)
-
Killing bacteria without denaturing proteins - (reply: 9)
-
immunohistochemistry and protein denaturation - (reply: 3)
-
How Conserved are the "Constant" regions of 'IgG' Heavy and Li - (reply: 5)
-
Protein is not binding to the ion-exchange column. - (reply: 4)
-
programme to predict amphipathic helices in a protein aminoacid sequence - (reply: 1)
-
How to tackle the problem of protein denaturation while storing? - (reply: 10)
-
Just a smear of proteins after ponceau red - Western Blot (reply: 4)
-
Storing tissue for protein and RNA extraction? - (reply: 13)
-
different western results when using acetone prep vs crude protein - (reply: 3)
-
Gap43 protein western Blot - (reply: 7)
-
post-translational modifications which increase molecular weight of the protein - (reply: 7)
-
SDS Gel - My protein bands go to the bottom of gel (reply: 9)
-
how to refold insoluble protein after purification - (reply: 10)
-
10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
-
how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
-
G protein inhibition - (reply: 3)
-
Isolation of golgi transmembrane protein - (reply: 4)
-
protein nature in different pH? - (reply: 3)
-
protein determination in loading buffer - (reply: 1)
-
oligos phosphorylation with pnk - (reply: 4)
-
problem in expression of target protein - (reply: 11)
-
concentrating low expression protein for western - will this work? (reply: 3)
-
Protein Refolding problem - (reply: 2)
-
Consistency problems with Micro BCA assay - (reply: 4)
-
Buffer Exchange & Protein Purification - (reply: 1)
-
Do I need to do BCA assay to quantify the protein before doing the ELISA? - BCA assay and ELISA (reply: 9)
-
storage buffer for purified protein - (reply: 3)
-
new techniques about protein ladder? - (reply: 3)
-
Solubilisation of proteins out of membranes - Which detergent at what concentration (reply: 1)
-
marker proteins for plasm membrane in c2c12 myoblasts - (reply: 1)
-
protein array - (reply: 1)
-
EMSA for non-DNA binding protein - (reply: 1)
-
who has done biotin pull down RNA interacting protein before? - biotin pull down RNA interacting protein (reply: 3)
-
Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
-
MAJOR PROBLEM IN EXPRESSING GST TAGGED PROTEIN - (reply: 1)
-
Problems with inconsitant protein recovery using ammonium sulphate (AS) precipit - protein precipitation/concentration (reply: 2)
-
Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
-
What is the best protein expression system - (reply: 1)
-
Big problem in GST Protein purification - (reply: 9)
-
Protein extraction from animal cells - (reply: 7)
-
preparing samples for phosphorylation sites identification - (reply: 3)
-
Trying to get protein out of insoluble fraction - (reply: 3)
-
getting rid of protein above 60 kDa - (reply: 7)
-
Suggested tool for protein analysis? - (reply: 4)
-
What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
-
Protein G purified IgG>>> extra band at approx 200 kd - (reply: 5)
-
How to stain protein fiber to view in light microscopy? - (reply: 1)
-
Protein reversed phase analysis.. linear or step gradients? - which is preferred and why?? (reply: 3)
-
protein isolation from cell lines - membrane bound proteins (reply: 3)
-
Protein Extraction - (reply: 1)
-
Can you get two proteins made from a heterozygous gene mutation? - (reply: 3)
-
Any good method for resuspension protein from TCA/acetone precip ? - (reply: 4)
-
SDS-page & lipidated proteins - (reply: 6)
-
Long PCR primers for epitope tagging - where to order? (reply: 3)
-
Separation of piperidine from proteins after AIC - (reply: 1)
-
IgG purification: Multimerization... - (reply: 4)
-
Protein crashing out of solution - (reply: 4)
-
Protein expression in E.coli DH5alpha? - (reply: 1)
-
question about protein molecular weight - (reply: 12)
-
Ab's production from recombinant proteins (2) - new idea (reply: 2)
-
how to identify the significance of a protein - (reply: 1)
-
Ab's production from recombinant proteins - (reply: 4)
-
how to define an appropriate protein reaction buffer? - (reply: 1)
-
Probelm with expression of ~85 kDa GSt fusion protein. - (reply: 5)
-
amount of protein for MALDI-TOF - (reply: 5)
-
Electro Elution of my protein after SDS-PAGE - (reply: 7)
-
Western for membrane bound protein - (reply: 11)
-
Size Exclusion Column - Proteins not coming out ! - BMP-2 (30 kDa) is inhjected in a colum (size exclusion at 100kDa) and (reply: 2)
-
Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
-
antibody against yeast protein - (reply: 1)
-
In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
-
My fusion protein's reading frame disrupted? - Please help...... (reply: 4)
-
Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
-
ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
-
Epitope tagging - Several basic questions (reply: 2)
-
Prtocol for large scale protein expression - (reply: 1)
-
Methods needed for gene/protein identification and function - (reply: 2)
-
detecting small membrane protein by western blot - (reply: 4)
-
losing protein expression after stablizing cells( please help) - (reply: 4)
-
Please help me! I am really tired of this stupid protein purification! - (reply: 8)
-
wetern for membrane associated protein - (reply: 1)
-
Protein Sample - How to store it? (reply: 1)
-
protein marker for 2D-PAGE - (reply: 2)
-
[In vitro Kinase assay] Do phosphorylated proteins migrate more in SDS-PAGE? - (reply: 3)
-
Measurement of protein- protein interaction? - (reply: 2)
-
HPV E7 protein - (reply: 1)
-
Rabbit/pig/human IgG Light chain amino acid sequence needed - (reply: 1)
-
IP first-timer - doubts on equal protein concentration control (reply: 2)
-
no protein expression - (reply: 2)
-
Protein stability in acetonitrile - storage conditions? - (reply: 1)
-
Sensitive protein assay - (reply: 2)
-
timing - DNA to RNA to Protein (reply: 1)
-
when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
-
protein band is shifted after incubation with plant protein extracts! - (reply: 2)
-
passage of cell culture and protein locolization - (reply: 1)
-
positive IgG in ChIP - (reply: 2)
-
What is the most effective method of protein precipitation? - (reply: 2)
-
protein precipitating - (reply: 8)
-
Dialysis doubt - (reply: 1)
-
protein estimation of monoclonal antibody - inconsistent values (reply: 2)
-
WB trouble - no signal of low kd protein - (reply: 5)
-
what's the difference between the purification of GST-fused protein and His- - (reply: 4)
-
validating the efficiency of inhibitors of protein kinases A & G - (reply: 3)
-
HELP estimation of protein in ammonium sulphate purified igG - (reply: 6)
-
Protein G beads for IP - (reply: 1)
-
please help: how to isolate nuclear protein from tissue samples - (reply: 1)
-
lysis buffer for coip with a membrane protein - (reply: 2)
-
Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
-
Antibody not binding to protein A/G - Immunoprecipitation (reply: 3)
-
Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
-
problem in centrifuge of protein - (reply: 3)
-
for protein refolding - (reply: 5)
-
protein kinase C (PKC) inhibitors - (reply: 1)
-
Size exclusion chromatography for 23 and 20.5KDa? - protein purification (reply: 3)
-
extract proteins for WB - (reply: 4)
-
TCA radiacive protein precipitation without lysis - (reply: 1)
-
How to chose vector and E.coli strain for protein expression - (reply: 2)
-
Methods for large-scale protein purification - (reply: 5)
-
Could protein modification block an antibody epitope? - (reply: 4)
-
How do i organize immunoblotting for 24 proteins from the same sample? - (reply: 4)
-
how to investigare new protein - (reply: 2)
-
protein extract questions - (reply: 1)
-
Yeast protein extract preparation for a quick western blot - (reply: 5)
-
Loss of LMW in Protein Standard - (reply: 2)
-
promoter regulation but not protein regulation - (reply: 4)
-
why express mamallian proteins in DH5alpha ? - (reply: 1)
-
Protein expression - (reply: 1)
-
Stability of protein back bone? - (reply: 6)
-
Problems experienced from adding 3xHA epitope to the C- terminus of some interes - Problems experienced from adding 3xHA epitope to the C- terminus of so (reply: 2)
-
ELISA-amount of total protein to coat wells - (reply: 2)
-
Protein extraction-Lysis Buffer - WB (reply: 1)
-
SOS for GST-fusion protein expression - (reply: 1)
-
Find a known domain a protein? - (reply: 3)
-
reuse of Protein G in IP procedure - (reply: 2)
-
plasmid prep with protein? contamination - (reply: 3)
-
Temperature effect of protein? - (reply: 5)
-
Protein loading control? - (reply: 4)
-
how can I get enough proteins for Mass spec? drive me mad! - (reply: 12)
-
Identify one protein among several - (reply: 4)
-
IgG purification - amonium precipitation method (reply: 5)
-
problem with overexpression - mRNA vs protein level (reply: 2)
-
3D protein structure - (reply: 2)
-
What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
-
phospho protein - (reply: 11)
-
IgG molecule - molecular weight (reply: 2)
-
antibody purification - how to get rid of bovine IgG? (reply: 3)
-
tips for detect phosphorylated proteins - (reply: 2)
-
transfected protein sequestered inside vesicles - (reply: 3)
-
Western Blots with (250 kDa) High Molecular Weight Protein - (reply: 2)
-
Trouble finding 5kDa protein in western blot - (reply: 5)
-
Larger sized Protein Standard Ladder Req - (reply: 1)
-
identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
-
GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
-
anti IgM secondary HRP? - (reply: 1)
-
cell lysis to investigate phosphorylated protein - (reply: 1)
-
protein ppt removes nucleotides? - (reply: 3)
-
How do you know if a specif Ser of the substrate protein is phosphorylated by a - (reply: 3)
-
isolation of protein complex by use percoll gradient - (reply: 1)
-
siRNA causing no protein knockdown - (reply: 1)
-
mass spectrometry analysis protein identification drive me crazy - (reply: 9)
-
How to renature denatured protein? - (reply: 4)
-
Separation of protein size of 20kDa and 150kDa - (reply: 4)
-
Can membrane proteins be separated using 2D-PAGE?!?! - (reply: 2)
-
Help--how to isolate the monomer proteins - (reply: 3)
-
Protein purification - (reply: 2)
-
40kDa protein for ladder - (reply: 4)
-
loading control for nuclear protein in rabbit cell extract - (reply: 1)
-
Vector for expressing proteins to be secreted - (reply: 3)
-
Protein Extraction from cell media for western blot - (reply: 3)
-
N-terminal protein sequencing.. "holes" at lysine residues.. meaning? - what kinda modifications cud've happnd? (reply: 2)
-
Western Blot for Higher molecular weight Proteins - (reply: 1)
-
HELP! contaminants do not separate from my protein during purification - (reply: 5)
-
Protein Expression Problem - (reply: 5)
-
How to measure protein expression levels of multiple forms of glycosylated prote - (reply: 2)
-
Protein from Liver tissue - (reply: 2)
-
Problem with Protein purification - Protein seems to stick to sepharose (reply: 6)
-
Estimating Protein Mass - Best way to quantitiate protein (reply: 1)
-
Phospho protein % in the cell - signal transduction (reply: 1)
-
Total proteins extraction - (reply: 2)
-
Epitope mapping - (reply: 5)
-
Surface proteins isolated by biotinylation are contaminated.. - (reply: 3)
-
membrane protein western blot - (reply: 3)
-
where can I access the all known proteins express in brain? - perhapes a database , but where to find it? (reply: 4)
-
Protein purification - (reply: 2)
-
Incomplete transfer of proteins for western blot - (reply: 4)
-
how to avoid cytosolic proteins in nucleus - (reply: 3)
-
Mammalian protein expresion in bacteria - Which plasmid and strain to use (reply: 7)
-
pET IPTG expression - Getting protein expression (reply: 1)
-
PRotein marker can give concentration of my protein in gel? - (reply: 4)
-
Gel shift with high isoelectric point protein - (reply: 1)
-
ChIP IgG has the same signal than the antibody tested - (reply: 9)
-
Protein lysis buffer for mass spec - recipe (reply: 15)
-
peptide phosphorylation analysis method - (reply: 3)
-
does low mRNA expression mean low relevant protein expression - looking for answers or references (reply: 9)
-
Hard to dissolve pelleted protein after TCA precipitation - Urgent!! Please check this out!! (reply: 2)
-
Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
-
Question for purchasing purified protein - (reply: 3)
-
Enough protein for mass spectrometry - How much is enough? (reply: 2)
-
Protein cleavage site? - (reply: 1)
-
MW marker for 150 kDa protein in Western blot - (reply: 5)
-
Correcting phospho-protein with total protein - (reply: 2)
-
Protein insoluble in 8M urea or 6MGuHCl - (reply: 8)
-
purification of proteins from sample - (reply: 12)
-
how can I dialyze protein through its PI and avoid precipitation? - (reply: 1)
-
questions on protein glycosylation - (reply: 2)
-
Immunoprecipitation & Mass Spec analysis - How much mg(ug) of protein lysate is needed for IP followed by Mass Sp (reply: 2)
-
dialysis tubes for chromatin purification - (reply: 5)
-
Protein expression. Question of V5 epitope and 6x His tag ! - (reply: 2)
-
EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
-
protein chromatography - (reply: 5)
-
Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
-
CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
-
CFP-protein - fuses proteins (reply: 1)
-
2D-PAGE PROTEIN ANALYSIS - SECRETED PROTEIN DESALTING (reply: 4)
-
Gel Loading- Equal Protein or equal volumes? - (reply: 2)
-
How to test the relationship between secreted protein and signaling pathway - (reply: 4)
-
loading control for mitochondrial protein - (reply: 1)
-
Protein expression Using NEB yest kit - (reply: 1)
-
GST fusion protein preparation protocol and troubleshooting - (reply: 1)
-
Protein Solubility - How to determine? (reply: 2)
-
E coli protein extraction for EMSA - (reply: 1)
-
Plant Protein Extraction - (reply: 2)
-
Chemiluminescent protein marker - (reply: 3)
-
Silver stain Protein detection - Silver stain problem (reply: 4)
-
Preventing protein aggregation - (reply: 2)
-
Method to determine whether protein exists as Monomer/Dimer etc.? - (reply: 6)
-
half life of EGFP protein - (reply: 3)
-
subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
-
Transfer optimization of membrane proteins to PVDF - (reply: 3)
-
protein precipitated - (reply: 2)
-
Phosphorylated Proteins - (reply: 3)
-
How to purify protein secreted in medium - (reply: 2)
-
Protein isolation - Small size His-tag Protein isolation (reply: 4)
-
Protein purification - (reply: 3)
-
protein extraction from SDS-PAG (GeBAflex) - your opinion about usefulness (reply: 1)
-
Why the DNA sequence big different but protein the similarity? - such as MAOA and MAOB genes (reply: 4)
-
Loss of phosphorylation after gel purification? - (reply: 3)
-
How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (reply: 2)
-
Retinoic Acid Receptors Protein Extraction - (reply: 2)
-
Protein purification - (reply: 3)
-
protein extraction from muscle biopsies - protocols needed (reply: 3)
-
Protein high molecular weight standards for gel filtration - (reply: 2)
-
How many methods can demenstrate to endogenous protein interactio? - (reply: 3)
-
Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
-
will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
-
Protein storage and BSA - (reply: 1)
-
A CoIP problem - How to reduce the non-specific binding of Protein A beads? (reply: 4)
-
SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
-
native protein - (reply: 2)
-
concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
-
Serum ELISA IgG titer quantitation - How to determine IgG titer without known sample? (reply: 4)
-
Does protein A affect Protein B level in my case? - need advice on my confusing result (reply: 7)
-
Specific and Total Activity for Protein Purification - (reply: 2)
-
Purified protein has no activity - (reply: 8)
-
Protein purification after cleavage (thioredoxin) - (reply: 3)
-
Isolating c. elegans protein free from bacterial's - (reply: 1)
-
CIP and protein - (reply: 2)
-
2D protein gel for miRNA target screening? - (reply: 2)
-
confusing shape of survivin protein band - (reply: 3)
-
protein concentration - bradford method (reply: 9)
-
size of tagged proteins - (reply: 3)
-
Which water is better for chemical preparation ? - water grade for protein purification (reply: 2)
-
Fusion protein without stop codon? - (reply: 1)
-
large protein degradation - during baculovirus purification (reply: 2)
-
truncated protein under normal conditions. - it is possible? (reply: 6)
-
how to recover my protein? - A terrible mistake (reply: 9)
-
Quantifying Proteins, help required! - (reply: 4)
-
His-tag protein expression problem - (reply: 4)
-
Protein A agarose for CHIP--pre-treatment is needed? - (reply: 2)
-
How do I prepare a sample to quantify a certain protein with ELISA? - (reply: 10)
-
specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
-
Immunostaining of cytoplasmic proteins in nucleus, why? - (reply: 10)
-
protein degradation - (reply: 2)
-
Recombinant IL-2 proteins.. - Work for cancer.. how? (reply: 1)
-
transfer gene to protein? - (reply: 3)
-
COS-7 and PC-3 transfected with a GFP-AR fusion protein - (reply: 4)
-
REFOLDING - visualisation of protein after denaturation step? - (reply: 1)
-
protein extraction from blood sample - (reply: 2)
-
Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
-
Expression in codon plus of GST fusion protein - (reply: 3)
-
Unreliable Protein Induction - (reply: 1)
-
2 questions in 1....about transferring large proteins...and blotting - (reply: 7)
-
Question about phosphorylation and ligation - (reply: 1)
-
Purification after oligos phosphorylation? - (reply: 1)
-
Questions about protein gel loading and immunoprecipitation - (reply: 4)
-
Formaldehyde Cross-Linking for protein - (reply: 1)
-
How to get rid of nuclease in protein preparation? - (reply: 5)
-
Why BSA can stabilize proteins? - (reply: 8)
-
EMSA with recombinant protein - (reply: 2)
-
CBB DYE BOUND TO PROTEIN - (reply: 2)
-
his-tag protein expression - inoculation of large cultures with plate colonies/ starter cultures vs. glycerol (reply: 1)
-
Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
-
protein precipitation upon dialysis - (reply: 5)
-
ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
-
faster proteins - (reply: 1)
-
Big protein - (reply: 6)
-
protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
-
in vitro translation - how is the protein purified? (reply: 12)
-
mobility of protein band over PAGE - please suggest (reply: 1)
-
getting rid of contaminant proteins in a Ni column using imidazole - (reply: 2)
-
PHOSPHORYLATION - (reply: 15)
-
precipitation of nuclear proteins during GST pull down assay - (reply: 1)
-
Protein concentration measure with NanoDrop - (reply: 22)
-
Protein extraction from soya meal using membrane - (reply: 1)
-
Strange: mRNA expression goes up but protein goes down - (reply: 2)
-
Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
-
Purified Protein Aggregation/Multimerization Problem - (reply: 3)
-
Protein analysis - (reply: 4)
-
Protein purification before mass-spec - need sample purification methods (reply: 4)
-
Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
-
what these components do in the buffer used in protein purification - (reply: 5)
-
protein folding- looking for renaturation buffer - (reply: 1)
-
Ultrasonic homogenizer for protein and enzyme extraction? - (reply: 3)
-
Low protein yield with immunoprecipitation - (reply: 3)
-
heating of samples during plant protein extraction - (reply: 2)
-
Protein extraction from SDS gel - (reply: 6)
-
Protocol for protein isolation from culture media - (reply: 3)
-
detecting transmembrane protein exoression for monoclonal ab production - (reply: 1)
-
acetone for protein precipitation - grade of purity (reply: 3)
-
SDS-PAGE protein aggregation and precipitation - (reply: 1)
-
Protein extraction/lysis from whole tissue - Do you have a protocol? (reply: 3)
-
Is ChIP is a good choice to find the DNA sequence for a binding protein? - (reply: 2)
-
too much protein for Western? - (reply: 5)
-
Low molecular weight protein problem - (reply: 11)
-
Binding partners to protein of interest - (reply: 1)
-
specific protein in high quantities - what can I do? (reply: 3)
-
How much can I believe protein MW standards? - (reply: 10)
-
Does SDS loading buffer + heat completely denature protein? - (reply: 3)
-
Fusion protein expressing problem - (reply: 2)
-
What is the maximum amount of protein that could be loaded in WB? - (reply: 2)
-
G protein coupled receptor - western blot - (reply: 1)
-
I want to strip the protein off PVDF membrane - (reply: 4)
-
GFP labelled protein purification - (reply: 1)
-
Periplasmic protein extraction problem! - (reply: 2)
-
Protein sample preparation for SDS-PAGE - (reply: 9)
-
Epitope tag positioning - (reply: 3)
-
Protein expression - decreased expression (reply: 2)
-
western - cells treated w/ protein synethesis inhibitor - (reply: 1)
-
microgram to micromolar conversion - Protein concentration - microgram to micromolar conversion - Protein concentration (reply: 4)
-
How to normalize the protein expression amongst different tissues - (reply: 7)
-
cancer-specific proteins - (reply: 1)
-
Maltose Binding Fusion Protein MBP - which strain for expression? (reply: 1)
-
can we purify the protein from westernblot membrane? - (reply: 5)
-
Total protein in human serum - (reply: 2)
-
PROTEIN AND DNA GEL ELECTROPHORESIS - GEL ELECTROPHORESIS (reply: 4)
-
amount of proteins isolated from cells - (reply: 2)
-
Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
-
protein preparation for Western Blot - (reply: 6)
-
Medium for adherent cells and serum/protein free - (reply: 2)
-
protein concentration measurement - (reply: 12)
-
Secreted protein degraded in conditioned medium? - (reply: 1)
-
How can I express my protein successfully? - (reply: 10)
-
how can i use SDS and Triton X-100 to isolate the membrane protein? - (reply: 6)
-
differential electrophoretic migration of phosphorylated proteins - (reply: 2)
-
can protein A agarose precipitate anti goat IgG? - (reply: 2)
-
Maltose Binding Protein - Information on maltose binding protein... (reply: 5)
-
protein ladder detection for western blotting - (reply: 2)
-
Detecting phosphorylated PKR - antibody and positive control - (reply: 4)
-
Selection of best recombinant protein produing strain - selection of industrial strain (reply: 3)
-
May a CARRIER-antibody compete between them for binding to protein G-agarose? - Immunoprecipitation problem (reply: 10)
-
cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
-
NF-kB EMSA - Different nuclear protein extraction method leads to opposite results (reply: 1)
-
thrombin cleavage and triton? - protein purification (reply: 2)
-
protein extraction - protein extraction from wheat seed (reply: 4)
-
How do you purify membrane protein? - help (reply: 2)
-
Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
-
Protein level in fibroblasts cell line - (reply: 1)
-
two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
-
protein precipitation - (reply: 4)
-
Protein immobilization on nitrocellulose membrane - (reply: 4)
-
protein insoluble in just about everything! - (reply: 3)
-
Protein in active form - (reply: 2)
-
SDS-PAGE - CAN I USED 12% GEL FOR WHEAT LEAVE PROTEIN SDS-PAGE (reply: 1)
-
Small fragment RNA isolation from interacting protein - (reply: 1)
-
MBP fusion protein... - (reply: 6)
-
Protein formed ppt after stored at -80oC - (reply: 3)
-
to check protein expression - (reply: 4)
-
Time point for reduction of protein levels after RNAi - (reply: 1)
-
quanitation of protein extracted with trizol - (reply: 7)
-
unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
-
trouble getting cysteine labeled with biotin-maleimide - cysteine scanning purified protein (reply: 1)
-
how to determine whether protein level increase is resulted from mRNA stability - (reply: 1)
-
Protein TCA Precipitation Resolubilization Question - (reply: 3)
-
Biotinilated proteins. Problems with Hens buffer - (reply: 2)
-
about protein domains in pfam - (reply: 4)
-
problem of protein expression - problem of protein expression (reply: 1)
-
Protein Transfection by Electroporation - (reply: 6)
-
sds-page of hemocyte lysate proteins - (reply: 2)
-
Protein expression troubleshooting - problem in expression of my protein (reply: 3)
-
Question about the strategy of protein purification - (reply: 4)
-
protein dialysis - (reply: 1)
-
Why should add DTT and what kind protein assay I should use - (reply: 4)
-
One or two bands with different protein extraction buffer - (reply: 3)
-
Membrane protein extraction - What about MEM-PER kit (Pierce)? (reply: 1)
-
Protein Structure - (reply: 4)
-
Protein forced aggregation - conditions? (reply: 1)
-
A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
-
Protein crystals - (reply: 3)
-
Overexpression of 630 aa protein - (reply: 3)
-
IP from frozen protein extracts - (reply: 10)
-
How to confirm a protein is a receptor? - (reply: 6)
-
Concentrate protein from dilute solution - (reply: 2)
-
Protein Precipitation (wessel and Fluege method) - 2 questions (reply: 7)
-
Cleaving a protein from GST - Troubleshooting methods to liberate my protein from gluthathonine sepharose (reply: 3)
-
protein purification -- 2 or more columns - (reply: 9)
-
protein folding - GST-fusion protein fluorescence (reply: 2)
-
what buffer will prevent the precipitation of protein at -20? - (reply: 5)
-
what is the best method to concentrate protein - (reply: 2)
-
protein for crystalization - (reply: 1)
-
How to convert the OD of protein to its concentration? - (reply: 2)
-
Protein structure comparison- sortware needed - (reply: 2)
-
problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
-
Develop a way to retrieve frequent protein segments by suitable algorithm - program, idea and so on . (reply: 1)
-
how to remove media components from protein solutions - (reply: 4)
-
Expression of fusion protein in mammalian cells - (reply: 2)
-
Membrane protein extraction and detection - General lab tech (reply: 1)
-
How to elute more protein from flag M2 beads(sigma)? - in hurry (reply: 6)
-
Cleavage of target protein from glutathione sepharose - (reply: 3)
-
After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
-
protein protein interaction - (reply: 3)
-
Perl script - parsing non-phosphorylated protein sequences from a Uniprot flat f - (reply: 12)
-
How does protein precipitate? - (reply: 1)
-
biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
-
Proportion of folding proteins among all 100 AA long proteins - (reply: 1)
-
detection of a secreted recombinant protein - (reply: 2)
-
IEF for pI 9.4 protein - ? (reply: 1)
-
general protein confimation in lysis buffer - effect of reducing agents (reply: 5)
-
protein precipitation during concentration - (reply: 5)
-
PEG to seperate Plasmide from Protein? - (reply: 2)
-
making large proteins - (reply: 6)
-
buffer for His-tag recombinant protein purification - (reply: 2)
-
Losing Low Molecular Weight Proteins in SDS-PAGE - (reply: 2)
-
Protein from blood samples - (reply: 5)
-
Aggregated proteins in SDS-PAGE - (reply: 5)
-
recommendation on mitochondria matrix protein antibody? - (reply: 1)
-
SDS protein sample over boiled, what happend? - (reply: 1)
-
how to purify fusion proteins on a large scale? - (reply: 2)
-
separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
-
how can I increase protein expression level - hurry (reply: 2)
-
concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
-
BCA protein assay - (reply: 3)
-
Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
-
how to do western with 500 kD protein? - (reply: 5)
-
stable or transient transfection method? - for protein expression (reply: 4)
-
Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
-
Transfer high molecular weight protein - (reply: 4)
-
his-tag protein - (reply: 7)
-
Determining if a protein is secreted - (reply: 12)
-
Protein denaturation and renaturation - (reply: 2)
-
His Tag protein purification - (reply: 3)
-
Protein deglycosylation - (reply: 1)
-
How to separate protein from nucleic acids? - (reply: 5)
-
Storing proteins - (reply: 2)
-
prediction of protein phosphorylation sites, how? - (reply: 4)
-
problem with the size of protein - (reply: 4)
-
Western after Ip- no IP protein, but .... - (reply: 1)
-
immunoprecipitation of nuclear protein - (reply: 2)
-
Cell line to be used for protein expression an purification - (reply: 8)
-
Protein expression problem - coexpression? (reply: 1)
-
Human IgA column - (reply: 1)
-
His tag protein purification problem - Binding of undesired protein (reply: 6)
-
protein diffusion in sds page gel - (reply: 2)
-
Protein disappeared after TCA precipitation - (reply: 7)
-
Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
-
Help:Total protein extraction from soil - (reply: 2)
-
low molecular weight proteins on SDS-PAGE - (reply: 1)
-
Using ECF for protein detection? - ECF instead of ECL? (reply: 1)
-
phosphorylated protein sequences with <20% homology - (reply: 5)
-
Big size proteins in SDS-PAGE - (reply: 3)
-
anyone working in protein aggregation..? - (reply: 6)
-
extracellular domain from a transmembrane protein, secreted or not? - (reply: 2)
-
How to make an antibody against my protein - (reply: 4)
-
eluting protein from the gel band... - (reply: 7)
-
transgenic mouse analysis - I have generated a transgenic mouse for a protein that is expressed in the brain (reply: 2)
-
Protein that doesn't express - (reply: 2)
-
Problems with protein extraction with Trizol - (reply: 1)
-
What is the name of substances that covalently binds proteins - (reply: 1)
-
protein/enzyme activity asssay - (reply: 1)
-
Protein Half-life determination - (reply: 1)
-
problems with expression of mutant protein - (reply: 1)
-
TCA vs. meth/chloro - concentration of proteins (reply: 3)
-
Protein migration in SDS gel - why? (reply: 2)
-
crosslink protein complex in vivo - (reply: 1)
-
problems whit protein solubility after purification - (reply: 5)
-
What can cause a change in protein level? - (reply: 1)
-
protein concentration - (reply: 1)
-
protein complex purification, what would affect? - (reply: 3)
-
protein extraction from tissue - (reply: 1)
-
protein isolation from brain material - yield? (reply: 8)
-
Which kind of post-translation modification can make protein size much bigger (3 - (reply: 8)
-
problems in concentration protein solution - (reply: 8)
-
Help! Protein extract degradation ? - (reply: 1)
-
what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
-
Concentration of secreted protein - ...step before IP.... (reply: 1)
-
Protein determination in a insoluble pellet - (reply: 6)
-
protein contamination in DNA electrophoresis - (reply: 2)
-
Quick help on determining protein size - (reply: 3)
-
Problem in protein preparation in 2-D electrophoresis - (reply: 1)
-
Stabilization of protein for injection in mice - when storing protein in freezer (reply: 2)
-
How to Draw a 3D structure of a Protein from its amino acid sequece? - (reply: 4)
-
Anybody that has used Microcon from Millipore to concentrate protein!? - (reply: 8)
-
protein purification - (reply: 5)
-
How to calculate protein size in Kilodaltons - (reply: 6)
-
problem with bacteriocin protein purification - (reply: 2)
-
protein precipitation / RNA binding factors - (reply: 3)
-
protein dialysis - i think i just cant understand it (reply: 15)
-
at which step can I store the protein? - purification from inclusion body... (reply: 3)
-
Phosphorylated proteins - (reply: 1)
-
How can I remove the Polyhistindine tag or myc epitope from my recombinant prote - (reply: 3)
-
RNA binding protein isolation - how to figure out what binds your mRNA? (reply: 1)
-
cloning membrane protein with 2 tags - (reply: 2)
-
purification problem. - most of my protein is washed away.... (reply: 10)
-
protein expression - (reply: 3)
-
why dose imidazole can elute protein from Ni-column - (reply: 3)
-
Outer membrane protein in the supernatant!? - (reply: 1)
-
WHat's the difference between Protein A and G? - (reply: 2)
-
Denature proteins on the membrane - (reply: 4)
-
loading protein onto SDS-PAGE gel - (reply: 2)
-
Protein precipitation using Methanol/Chloroform - (reply: 1)
-
Protein yeast extraction for Western blot - (reply: 3)
-
Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
-
Dialysing the proteins - (reply: 3)
-
which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
-
western protein ladder - (reply: 9)
-
How much Antibody did I get? - Protein G column (reply: 1)
-
suggest protein purification methods? - (reply: 2)
-
phosphorylation analysis - (reply: 2)
-
Please recommend a fast way to get purified protein - (reply: 2)
-
hesitation between Rasmol and protein explorer - software (reply: 2)
-
DNase I......how much to use in units? - protein purification issues (reply: 1)
-
protein extraction from stable mammalian clones - (reply: 8)
-
Stains for proteins.. - Silver v/s Coomassie (reply: 1)
-
Need help in Using BLAST for beginner - finding the paralog, ortholog and protein (reply: 1)
-
protein purification - (reply: 1)
-
Membrane protein purification - (reply: 1)
-
question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
-
native gel -- protein migration - (reply: 1)
-
Measure OD600 when perform the protein expression - (reply: 6)
-
Differences Between Hormones - difference between the action of protein and steroid hormones (reply: 1)
-
Smearing of protein samples from whole tissue lysates - (reply: 6)
-
Protein Concentration Methods - (reply: 7)
-
Protein transfer, high resistance causing loss in voltage? - (reply: 2)
-
Do repeated freeze-thaw cycles affect protein activity? - (reply: 4)
-
How to analysis glycated proteins on SDS-PAGE - (reply: 1)
-
General equation for protein degradation - (reply: 1)
-
His-tag fusion protein detection problem - (reply: 2)
-
Separating DNA and nucleotides from protein - (reply: 4)
-
many bands other than my protein on western... - please help me to explain this... (reply: 5)
-
protein precipitation for mouse liver - (reply: 1)
-
bee venom peptides, proteins, enzymes - (reply: 1)
-
Protein elution question - (reply: 1)
-
Protein purification from E Coli - (reply: 11)
-
membrane protein western blot - (reply: 2)
-
Western/Signaling Studies - How to get rid of background phosphorylation? - Western/Signaling Studies - How to get rid of background phosphorylation? (reply: 3)
-
big black spot in the place of my protein on western... - (reply: 4)
-
PVDF versus nitrocellulose - which is better for simple Western? - "normal" protein of 13kDa (reply: 4)
-
Protein degradation - (reply: 1)
-
extract protein from SDS PAGE - (reply: 1)
-
detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
-
Nuclear protein in a post nuclear supernatant? - (reply: 6)
-
How to stop protein from preciptating during dialysis - (reply: 4)
-
Phenol chloroform extraction DNA: protein contamination - how to remove all protein from my DNA? (reply: 1)
-
Liver tissue lysis for ER protein Western blotting - (reply: 2)
-
Calcium homeostasis and protein - relationship between calcium homeostasis and some proteins? (reply: 3)
-
38kda protein appearing as smear on western - (reply: 5)
-
sds page of protein in tfa & acotonitrile - (reply: 1)
-
protein glycosylation analysis - (reply: 3)
-
Protein purification - (reply: 3)
-
Looking for genomic targets of a DNA binding protein. - (reply: 1)
-
Imidazole in column elution - Removing Imadazole from purified protein without dialysis (reply: 1)
-
How to get DNA binding protein in the clear supernatants? - (reply: 8)
-
Expected protein size ? - fusion protein-size in kDa? (reply: 8)
-
problem transfering small proteins - (reply: 4)
-
any idea about running small size of protein? - (reply: 6)
-
DNA amount for protein expression - protein His-tag (reply: 1)
-
Knockdown protein expression using rnai - (reply: 4)
-
SIZE OF PHOSPHORYLATED PROTEINS - (reply: 6)
-
Blotting time and voltage for a 20kd protein - (reply: 4)
-
unwanted protein degradation - question (reply: 1)
-
How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
-
protein loss in sterile filtration - suggest the 0.22 micron filter (reply: 4)
-
isolation of protein from blood - isolation of protein from blood (reply: 1)
-
Porblem using streptavidin coated beads - Using streptavidin coated beads to purify proteins tagged with a biotinylated ol (reply: 2)
-
protein expression in mammalian cell - (reply: 1)
-
How to seperate protein and carbohydrate from a mixture - (reply: 6)
-
[32] phosphorylation hints needed! - (reply: 1)
-
protein loss during nickle clolumn purification - is there always a lot loss of protein during column purification? (reply: 2)
-
Shipment of extracted proteins - (reply: 3)
-
membrane protein extraction from dissected thymus - (reply: 1)
-
How to calculate the size of a protein (in kDa)? - (reply: 1)
-
Protein extraction - (reply: 1)
-
protein ppt using acetone - (reply: 1)
-
Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
-
What is the best way to identify a protein isoform? - (reply: 4)
-
need ideas for protein protein interaction - protein interaction assays (reply: 3)
-
way to confirm that my protein is active - (reply: 4)
-
Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
-
protein synthesis - (reply: 2)
-
How amido black stains protein? - (reply: 1)
-
GST protein purification - only 3% bind to beads (reply: 6)
-
Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
-
why get rid of imidazole in his tag protein purification - (reply: 3)
-
Protein expresses wrong size - (reply: 5)
-
What is the best nuclear protein marker? - Fractionation (reply: 3)
-
renilla Luciferase and protein concentration - (reply: 1)
-
Protein sequence comparison report - (reply: 2)
-
Help RNA and protein extraction - i dont understand the chemistry behind it (reply: 3)
-
his tag protein not expressing - I am stuck !!! (reply: 6)
-
6 His tagged protein purification - (reply: 1)
-
PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
-
Trouble expressing a protein in transient transfection - (reply: 2)
-
Problem resuspending precipitated protein - (reply: 2)
-
How to prevent the protein degradation - protein degradation (reply: 2)
-
loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
-
trouble in scaling-up protein expression - (reply: 5)
-
How to measure labelled protein concentration? - (reply: 1)
-
His-tag protein purification - (reply: 1)
-
Human IgG from protein G - (reply: 1)
-
Same protein, different size on Western and IP ? - (reply: 2)
-
imidazole in protein purification - (reply: 2)
-
Western transfer time: Is it crucial for big proteins? - anomaly HELP (reply: 2)
-
protein dephosphorylation by alkaline phosphatase? - (reply: 8)
-
problem in expression of his tag protein - (reply: 6)
-
Strange protein transfer - (reply: 3)
-
IP the membrane protein - How to elute the protein complex from the beads (reply: 2)
-
anti-c-myc-agarose protein purification - (reply: 1)
-
Phosphorylation with PNK - (reply: 5)
-
pure protein - .. (reply: 4)
-
protein extraction from leaf - (reply: 4)
-
Quantify protein expression - (reply: 1)
-
which tag and vector is the best for expressing protein in bacteria? - (reply: 4)
-
CHIP with Tagged Proteins - (reply: 2)
-
question about natively seperation of subunits of complex protein by PAGE - (reply: 1)
-
How to quantify fluorescent-tagged protein - (reply: 2)
-
help - small proteins and western blotting - (reply: 23)
-
protein extraction efficiency - protein extraction efficiency (reply: 3)
-
Loss of proteins during Western transfer - (reply: 4)
-
Aggregation of protein after purification? - (reply: 2)
-
protein isolation of plant tissue -trizol regent - (reply: 3)
-
gene expression vs protein expression - (reply: 3)
-
Protein analysis of conditioned media - How to prepare media before SDS-PAGE? (reply: 4)
-
Phosphorylation - (reply: 8)
-
How to detect receptor on the cell surface? - using the less viral protein possible (reply: 1)
-
codon bias? - protein expression (reply: 11)
-
Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
-
Protein rofolding problem - percipitation (reply: 4)
-
sonication free protein extraction... - (reply: 2)
-
Effect of APS (Ammonium persulfate) to protein - (reply: 1)
-
protein did not enter the gel on EMSA - (reply: 2)
-
6 his tagged protein purification - purification (reply: 2)
-
ELISA with denaturated proteins? - (reply: 6)
-
Expressing GST-Fusion protein - Are larger protein inserts harder to express? (reply: 4)
-
Protein samples still in liquid form at -20C - Storage condition for proteins (reply: 8)
-
protein purification - (reply: 3)
-
Does imidazole absorbe at 280 nm? - How do you measure your protein content? (reply: 4)
-
the range of protein amount for loading - western blotting (reply: 4)
-
optimal SDS separation protein - (reply: 3)
-
phosphorylated oligos and vector - (reply: 2)
-
Protein Structure Prediction - HELP!! (reply: 4)
-
PQE vector - Getting protein in its soluble form (reply: 3)
-
sample preparation for IEF - trouble in normalizing protein (reply: 1)
-
Purifying a membrane protein - - the trouble with lipids? (reply: 3)
-
determining protein expression level from a Western!!! - (reply: 6)
-
Help with concentrating proteins in culture medium - (reply: 4)
-
WB- proteins of lower-than-expected MW - (reply: 1)
-
Making solutions for protein prep - (reply: 3)
-
shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
-
protein sizes - which tools (reply: 1)
-
How much a protein converted into a crystal? - crystallization (reply: 2)
-
How to make pre-stained protein marker in lab - (reply: 1)
-
How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein (reply: 3)
-
Yeast Fluorescent Proteins - Yeast Fluorescent Proteins (reply: 1)
-
What is the role of integral membrane proteins in signaling? - (reply: 1)
-
brain protein isolation for WIB - can I simply use 4xLB+BME? (reply: 2)
-
GFP protein not soluble? - (reply: 1)
-
Protein hydrophobicity - (reply: 1)
-
Expressing protein in a soluble form - What should be the next logical step? (reply: 8)
-
Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
-
MBP tagged protein not bind resin - (reply: 8)
-
cDNA size VS protein Size - MW weight confusion (reply: 7)
-
Acetyl coenzyme A and dialysis - Removing AcCoA and dialysis problem (reply: 2)
-
Protein Expression - Question (reply: 1)
-
aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
-
Protein interaction - (reply: 3)
-
Stability of protein in powdered leaf sample - Protein stability after quick freeze in liquid N2 (reply: 1)
-
Protein not penetrating the resolving part of the gel - (reply: 2)
-
Does SUMO fusion protein increase solubility? - (reply: 2)
-
<10kD protein in Western - (reply: 3)
-
Mitochondrial, cytosolic and Nuclear Protein extractions - An all in one protocol? (reply: 1)
-
whether someone know about such a kind of proteins - (reply: 1)
-
Proteins and marker are not running down gel - Protein purification (reply: 7)
-
prepartion of membrane protein - (reply: 3)
-
About pH and pI for his-tagged protein purification - (reply: 2)
-
Homemade DNA/Protein A beads - i think i made a boo-boo (reply: 2)
-
Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
-
Methods of proteins extraction from plants - protein extraction industry (reply: 1)
-
glycerol is still there after dialysis - (reply: 1)
-
Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
-
Protein isolation for western - (reply: 8)
-
PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
-
Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
-
Can antibodies and proteins be vortexed? - (reply: 4)
-
aggregates in SDS-PAGE? - double mw of proteins in SDS-page (reply: 2)
-
in-vivo method of identifying 2 protein binding? - (reply: 3)
-
quantify protein on beads? - (reply: 4)
-
"Help" : how to identify of proteins binds to specific DNA - (reply: 3)
-
Proposal for strategy to determine 2 protein interactions? - suggest sugges! (reply: 11)
-
western of a big protein - (reply: 2)
-
Insect cell sonication protocol? - Protein Purification (reply: 1)
-
Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
-
How to solve a protein when not soluble in 6M GuHCl? - (reply: 1)
-
GST proteins detection - (reply: 1)
-
A question about cell stain: fix the cell have fusion protein - (reply: 1)
-
Help on protein integrity Western Blot - Sample preparation and detection (reply: 6)
-
how quick the protein get degraded in room temp - (reply: 6)
-
DNA "clean-up" - How to get rid of DNA contaminants: RNA, proteins (reply: 1)
-
STAT 1 / phospho STAT 1 - can't detect my protein (reply: 3)
-
Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
-
Protein sticking to membrane - Protein concentration woes (reply: 1)
-
His-tagged protein binding problem - Protein Binding (reply: 1)
-
a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
-
problem with DNA extraction - can't get rid of protein phase (reply: 3)
-
BCA assay - (reply: 2)
-
Protein consensus pattern (motif) database - (reply: 1)
-
IgG's "epsilon" - (reply: 2)
-
protein quantification- triton - (reply: 3)
-
phosphorylation - (reply: 5)
-
what generally methods to improve the protein solubility - (reply: 1)
-
solubility of protein - (reply: 1)
-
NP40 concentration in protein lysis buffer - (reply: 1)
-
help to see whether my protein is imported to the nucleus - please give some advice (reply: 5)
-
protein quantitation - for equal western loading (reply: 2)
-
Protein Homogenization - Soluble and insoluble protein (reply: 3)
-
Protein produced with brown color? - I have some problem in protein expression (reply: 1)
-
His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
-
recombinant protein lost immunological activity - (reply: 1)
-
more questions about protein expression... - (reply: 1)
-
Express a vaccine protein - (reply: 1)
-
Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
-
Protein with His6 tag - (reply: 2)
-
Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
-
Protein degradation - Recombinant protein degradation in insect cells (reply: 1)
-
isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (reply: 1)
-
Removing Exonuclease From Protein Prep - How can I remove exonuclease from a protein prep (reply: 4)
-
Cleaved GST fusion protein retained on beads! - Affinity of GST-cleaved protein for glutathione sepharose (reply: 7)
-
the protein band disappear in the second dimension - 2D-Gel (reply: 2)
-
antibodies to epitope tags - (reply: 1)
-
protein precipitation after IMAC - protein purification (reply: 1)
-
measuring protein concentration - (reply: 5)
-
Solubility ribosomal proteins - Solubility ribosomal proteins (reply: 4)
-
Protein extraction from rat liver tissue - (reply: 1)
-
Protein purification - Problem with his-tag protein gel filtration (reply: 5)
-
Have any one know about Protein A - (reply: 2)
-
neomycine resistance protein antibody - does anyone exist? (reply: 2)
-
Protein expression problems - (reply: 4)
-
Bacterial Periplasmic protein localization - B-lactamase- alkaline phosp Fusion experiments (reply: 4)
-
quesiton about GST fusion protein - (reply: 1)
-
How to predict the 3-D structure with the sequence of protein? - (reply: 4)
-
Removal of endotoxin from proteins & DNA - Protein & DNA purification (reply: 15)
-
problem with expression of GST fusion protein - (reply: 1)
-
protein purifiacation - (reply: 1)
-
trizol extraction for phospho proteins - (reply: 4)
-
aggregation of purified proteins - (reply: 4)
-
How imidazole can be washed off the Ni-NTA agarose beads - reuse of Ni-NTA agarose beads for His protein (reply: 2)
-
total protein in Western blotting - (reply: 1)
-
Problem in protein expression - (reply: 5)
-
Protein reduction without mRNA reduction? - (reply: 1)
-
UV (280nm) Protein Quantification - Rough estimation of [protein] does it work? (reply: 1)
-
let me have a simple test for detecting phosphoproteins - (reply: 5)
-
Binding of protein to immunoassay plates - binding in denaturing conditions (reply: 1)
-
Protein solubilization and Western Blotting - (reply: 1)
-
check protein expression from tissue - (reply: 1)
-
Protein Structure - (reply: 1)
-
Phosphorylation not affecting SDS-PAGE mobility - (reply: 7)
-
remove BSA from IgG prep - (reply: 2)
-
losing protein activity - (reply: 3)
-
Extraction of membrane proteins and western on them - (reply: 3)
-
I forgot to pretreat my dialysis membrane ! - (reply: 1)
-
Who is my closest gene relative? - Help to determine phylogeny of a protein (reply: 2)
-
protein purification - his-tag protein purification (reply: 2)
-
choosing insect cell for high-cystine protein expression - (reply: 1)
-
SDS-PAGE question - seperate protein (reply: 3)
-
help with protein size - western blot (reply: 1)
-
protocols for mouse cells - splenocyte preps, protein extraction (reply: 1)
-
RNA/protein Isolation - Is there some good KIT or ...? (reply: 3)
-
problems with loss of purified protein due to precipitation - protein purification (reply: 4)
-
Phosphorylation of protein - Trouble with backphospho (reply: 3)
-
protein lysates preparation - (reply: 4)
-
Protein expression troubleshooting - (reply: 2)
-
protein expression - How to express protein of a high molecul (reply: 4)
-
293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
-
question about SDS-PAGE - electrophoresis of proteins (reply: 4)
-
Purification of nuclear proteins - (reply: 2)
-
TCA precipitation of proteins - (reply: 15)
-
western blotting of expressed protein - (reply: 1)
-
protein expression in Pichia pastoris - (reply: 91)
-
help with protein expression and detection - (reply: 4)
-
ionic Charge and pI of protein information!? - Proteomic (reply: 2)
-
isolate secreted proteins - (reply: 3)
-
Protein concentrators - (reply: 1)
-
DNA binding proteins - (reply: 3)
-
Dot blot on bacteria - screen of protein expression (reply: 1)
-
western blot for membrane protein with his-tag - can't detect with anti-his tag Ab? (reply: 3)
-
FORTE protein profile¨Cprofile comparison tool - (reply: 1)
-
Extracting Protein Using TriZOL - Is it possible? Are there problems? (reply: 3)
-
Triton X-100 - solubilization of proteins (reply: 1)
-
Protein Expression - (reply: 1)
-
concentrate protein in culture media? - (reply: 2)
-
Detection of low abundant proteins with IHC - (reply: 1)
-
Protein pellet solubilisation - (reply: 4)
-
His purification of a regular expressed protein - (reply: 1)
-
protein precipitation - protein precipitation (reply: 5)
-
Spec problems - Trying to measure protein conc. (reply: 2)
-
tissus proteins extraction - (reply: 3)
-
Hydrophobic proteins extraction from yeast wall cell - How to extract hydrophobic proteins? (reply: 1)
-
Protein extraction protocol - (reply: 1)
-
Protein assay - Percoll containing samples (reply: 1)
-
total protein assay in cell extracts - (reply: 3)
-
Yeast protein extraction - need to change my expression system... (reply: 3)
-
Precipitation of Native Protein - IMAC of Native Protein (reply: 1)
-
Autoradiography for proteins - Radioactive protein probes (reply: 1)
-
protein precipitates after dialysis - Protein purification (reply: 4)
-
PROTEIN ASSAY - LISTING ALL THE PROTOCOLS FOR ASSAYING IMPORTANT PROTEINS (reply: 2)
-
PROTEIN ASSAY - MEASUREMENT OF PROTEIN IN CELL LYSATES (reply: 2)
-
How to extract hydrophobic proteins from yeast wall cells? - (reply: 1)
-
ELISA - direct protein assay with ELISA (reply: 1)
-
Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
-
electroelution of protein fromsds-page for use in immunization - (reply: 2)
-
IgM Purification - (reply: 1)
-
IgM hypermutation - (reply: 1)
-
about IgG - (reply: 1)
-
Protein extraction from humantissue - (reply: 1)
-
problem with trizol - solubilization and western of proteins (reply: 2)
-
Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
-
his-tagged protein purification - (reply: 1)
-
protein express - (reply: 1)
-
Sigma nuclear protein extraction - (reply: 1)
-
How to concentrate protein from culture medium - for Western blot (reply: 1)
-
Plant Protein Extraction - (reply: 1)
-
Flag Tagged Proteins - (reply: 1)
-
Polysaccharides co-precipitatingwith protein in ammonium sulfate - (reply: 1)
-
Protein extraction form gels - (reply: 2)
-
electroelution - precipitate on dialysis (reply: 3)
-
Conservation GST fusion proteins - (reply: 1)
-
Supernatant proteinprecipitation - (reply: 1)
-
Bradford Protein Assay - (reply: 2)
-
Protein size ! - (reply: 6)
-
SDS-PAge problem! - Protein separation (reply: 5)
-
Protein extraction from bacterial cells - Suitable protocol (reply: 1)
-
membrane protein purification - how to pufify cd3 protein from jurkat cell (reply: 1)
-
protein expression - What is a suitable strain (reply: 1)
-
proteins electrophoresis - (reply: 2)
-
how to precipitate the nucleicacid with out proteins - (reply: 2)
-
Hydrophobic proteins extraction? - (reply: 1)
-
Bradford Protein Assay - (reply: 1)
-
crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)
-