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Protein Related Discussions
  1. Unstable protein - (reply: 1)
  2. Seperating High MW proteins - (reply: 2)
  3. Disappearing protein MWM marker - (reply: 1)
  4. Purification of GST FUSION PROTEIN - (reply: 1)
  5. Protein isolation by Trizol - (reply: 1)
  6. Can i send protein band from SDS-PAGE for MAlDI......... - What could be the complication!!!!!!!!!! (reply: 5)
  7. protein in inclusion bodies-plz help - (reply: 4)
  8. Protein Loading and Western Blot - Western Blot (reply: 5)
  9. single stranded binding protein? - how to put into my PCR reaction? (reply: 6)
  10. problem in protein expression after 8 months - (reply: 2)
  11. mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
  12. Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (reply: 1)
  13. Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
  14. GST protein purification - (reply: 1)
  15. Removing all cell surface proteins/receptors - (reply: 3)
  16. Pioneers of Mutagenesis to Probe Protein Structure and Function - (reply: 1)
  17. heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
  18. western blot: why are proteins not separated? - (reply: 4)
  19. protein concentration - (reply: 4)
  20. troubles with small proteins (less than 20) on SDS page - (reply: 5)
  21. protein spreading in gel - (reply: 1)
  22. no expression of my target protein - (reply: 3)
  23. 2D gelelectrophoresis: prestaining of proteins - (reply: 4)
  24. general protein purification from mouse tissue - (reply: 4)
  25. Different intensity present in reference protein even though BCA performed - (reply: 1)
  26. Technique to estimate the level of protein expression from media - (reply: 2)
  27. unexpected 40kDa band -E.coli protein expression - (reply: 3)
  28. Protein purification by two stage chromatography - MDH by anion exhange & gel filtration (reply: 1)
  29. Protein preicipitated after Dialysis - (reply: 7)
  30. Methods of concentrating Protein lysate - (reply: 2)
  31. DNA motif database / no TF - database of DNA motifs for binding of DNA replication proteins (reply: 2)
  32. 1% boiling buffer for protein extractions - (reply: 3)
  33. Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
  34. Gene complexity and protein complexity analysis - (reply: 2)
  35. protein extraction from mouse brain tissue - (reply: 1)
  36. pROTEIN DOMAINS - new to proteomics (reply: 3)
  37. Protein identification - (reply: 1)
  38. Phosphatase-Protease inhibitors in TCA total protein extraction - (reply: 2)
  39. protein on membrane - (reply: 1)
  40. protein concentration problem - (reply: 2)
  41. protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
  42. Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
  43. temperature can effect the protein active? - (reply: 1)
  44. storing dialysis tube(bag) - (reply: 2)
  45. HIS TAG Protein Estimation - (reply: 5)
  46. precipitation of protein with acetone - (reply: 3)
  47. Protein Conc using Bradford Assay - (reply: 1)
  48. optimal timepoint to collect secreted protein - (reply: 1)
  49. Determining if a protein is a downstream target of another protein - (reply: 4)
  50. Fast Protein Liquid Chromatography (FPLC)/ HPLC - (reply: 4)
  51. protein solubility - (reply: 2)
  52. A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
  53. Advantages of Proteomics Study - (reply: 1)
  54. Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
  55. total protein extraction from brain tissue - (reply: 3)
  56. smeared proteins western blot - (reply: 5)
  57. Protein come from nowhere - we cannot find RNA (reply: 5)
  58. Do we have fluorescence genes? - genes which code for fluoscent proteins (reply: 12)
  59. what cell line to use for visualizing membrane localized protein - (reply: 1)
  60. DNA "snot" in cell lysis/protein extraction - (reply: 6)
  61. Protein insolubility problem after bacterial induction - (reply: 2)
  62. TAT protein - (reply: 1)
  63. Comparing surface protein expression of different cell lines - (reply: 1)
  64. Protein elution problems - (reply: 2)
  65. Comparative Genomics, Protein Sequencing, more - (reply: 1)
  66. finding a protein's function - (reply: 1)
  67. Negative Control Rabbit IgG for ChIP Assay - (reply: 3)
  68. DNA Protein pull down protocol for mass spec - (reply: 2)
  69. Protein ladder - (reply: 8)
  70. amount of protein to load for a western? - (reply: 2)
  71. protein absorbance wavelength? - (reply: 1)
  72. Protein size prediction - (reply: 4)
  73. Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
  74. Coating Protein A and G on ELISA plate - (reply: 2)
  75. Huge mRNA overexpression, but no protein overexpression. - (reply: 10)
  76. about transfer low molecular weight protein in tricine PAGE - (reply: 5)
  77. protein isocratic HPLC.. preparatin of tris buffer + n-propanol mix - getting variations in Retention time.. (reply: 1)
  78. 19kda-15kda-6kda.. resolution of these proteins? - which gradient sds-page would be best suitable? thanks! (reply: 2)
  79. Protein expression problems in HB101 - (reply: 1)
  80. protein expression problem - (reply: 1)
  81. protein extraction protocol? - (reply: 3)
  82. Identifying a protein isoform - (reply: 1)
  83. Trying to detect correct membrane protein size in gel - At wits end (reply: 4)
  84. transfer problem - i cannot transfer proteins to a pvdf membrane (reply: 7)
  85. isotope labeling of proteins - (reply: 4)
  86. TRIZOL for protein extraction - any problem? (reply: 4)
  87. Elution problem in Protein G column - Guide me (reply: 2)
  88. proteins expression - (reply: 1)
  89. Experience with protein G dynabeads? - (reply: 2)
  90. Protein Informative Tools - (reply: 1)
  91. URGENT: software tools for proteomics - (reply: 1)
  92. Why Proteins? - Can't figure proteins out, let's find some help? (reply: 4)
  93. DMSO for protein stability at -80oC - protein storage (reply: 4)
  94. How to separate very close protein bands - (reply: 5)
  95. size exclusion.. how to reduce tailing. for proteins.. - sillica matrix tsk columns.. (reply: 2)
  96. oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
  97. getting part of a protein sequence - finding protein domain sequences (reply: 1)
  98. purification of non his-tagged proteins - (reply: 4)
  99. Sample buffer and protein assay - (reply: 1)
  100. protein extraction fromn soil bacterium - (reply: 1)
  101. how to find the modification enzyme of a target protein - (reply: 1)
  102. Residues don't match... why? - in human and mouse proteins (reply: 2)
  103. How many ways are there to conc. protein? - (reply: 5)
  104. 6XHistaq proteins - (reply: 2)
  105. minimum protein conc for immunoprecipitation - what's the minimum needed for results (reply: 1)
  106. Seperation of unconjugated protein after hapten conjugation - (reply: 1)
  107. Protein purification - (reply: 5)
  108. Protein Extraction from SDS-PAGE - (reply: 1)
  109. probing one protein with multiple antibodies - (reply: 2)
  110. Acid hydrolysis of protein - (reply: 1)
  111. Subcellular localization of -HA tagged protein - (reply: 1)
  112. DNA binding proteins ELISA - need suggestions (reply: 1)
  113. Western for a 500kda protein - some troubles, need help! - (reply: 1)
  114. Inclusion bodies protein determination - (reply: 1)
  115. How to make my protein Soluble? - Bacterial system it sucksss ? (reply: 1)
  116. Protein homology modelling - (reply: 3)
  117. wrong protein size - (reply: 6)
  118. Mysterious protein degradation - (reply: 4)
  119. protein extraction from yeast--all extracted proteins are below 50KD - (reply: 1)
  120. Acid in Dialysis Tubing - probably a stupid question (reply: 2)
  121. Which control protein for Western Blotting of nuclear protein? - (reply: 9)
  122. Protein sequence alignment - (reply: 4)
  123. Protein isolation from RNAlater stored tissue - (reply: 5)
  124. How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
  125. Point Mutation, Protein Folding, Base Pairing - (reply: 3)
  126. Protein extraction from periplasmic space - a simple protocol anyone? (reply: 1)
  127. membrane protein extraction - membrane protein extraction (reply: 7)
  128. membrane protein and boiling - (reply: 3)
  129. protein band is shifted after boiling sample with sample buffer - (reply: 7)
  130. Dialysis, how long? - (reply: 8)
  131. Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
  132. Removing histone proteins from nuclear protein isolation - (reply: 2)
  133. Bradford Assay - Calculation protein concentration (reply: 9)
  134. Which quantity of purified protein in WB? - (reply: 1)
  135. Protein questions - (reply: 6)
  136. Elisa- need help - Elisa - Phosphorylation studies (reply: 1)
  137. Dialysis Buffer for Recombinant Protein - (reply: 5)
  138. protein recovery problems in analytical size exclusion chromatogrphay - or how do i have a more sensitive method? (reply: 3)
  139. to explain why i see two forms the same protein in SDS-PAGE - (reply: 1)
  140. Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
  141. Frozen Protein A agarose beads - (reply: 1)
  142. protein inclusion bodies.. purpose of multiple water washes after lysate centrif - (reply: 5)
  143. protein degredation - (reply: 3)
  144. protein purification from yeast - (reply: 2)
  145. Problem in concentrating protein - (reply: 1)
  146. Reverse phase chromatography - protein sample preparation and loading - (reply: 1)
  147. protein absorption at 260nm - (reply: 7)
  148. can't view the protein band..help! - (reply: 1)
  149. unstable protein expression,pleeeeeese help - (reply: 6)
  150. cleaving protein - (reply: 8)
  151. protein estimation colorimetry.. preferred range.. - statistically more precise & accurate? (reply: 2)
  152. Recombinant protein stability - stability issue (reply: 1)
  153. Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
  154. How to find miRNAs for one designated protein? - miRNA (reply: 1)
  155. transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
  156. protein normalizer (equal protein loading) - (reply: 3)
  157. ddephosphorylating proteins using acid phosphatase - (reply: 1)
  158. Fishin out possible interacting protein for known RNA..?! - (reply: 1)
  159. Protein extraction supernatant turns black/sooty color - (reply: 4)
  160. Protocol for IgG Purification from Ascites? - (reply: 1)
  161. experimental determination of protein function - (reply: 2)
  162. How to separate proteins of equal size - (reply: 3)
  163. Using urea in ion exchange chromatography of proteins - (reply: 1)
  164. upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
  165. protein tag for ICC/IF - (reply: 2)
  166. cytosolic protein collection - (reply: 3)
  167. Is there a way to screw up dialysis? - (reply: 8)
  168. protein extraction from Daphnia/rotifera - (reply: 3)
  169. immobilized protein affecting activity - (reply: 4)
  170. Oligomeric protein - how to measure concentration? - (reply: 2)
  171. his-tag protein pure but degradated during growth - his-tag protein pure but degradated during growth (reply: 15)
  172. precipitation of guanidine HCl-sample after adding 4X laemmli buffer - SDS-PAGE protein sample preparation (reply: 2)
  173. Protein expression changes after freezing+thawing - (reply: 1)
  174. to cross-link a protein to a polysaccharide, which to choose? Sulfo-NHS or NHS? - (reply: 5)
  175. programmes for protein sequences characterization - (reply: 1)
  176. band size with flag tagged proteins? - (reply: 3)
  177. identification of secretory protein - (reply: 1)
  178. Immunization with protein band from SDS-PAGE - (reply: 5)
  179. protein extraction - cytosolic lysis buffer (reply: 8)
  180. protein elution - any idea to elute protein (reply: 5)
  181. caspase-3 cleavage assay - how to test the clevage of a protein by caspase in vitro (reply: 2)
  182. what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
  183. protein concentration - (reply: 1)
  184. bands sizes with sectreted protein - (reply: 1)
  185. smearing of proteins during transfer - (reply: 10)
  186. contamination of DNA in protein sample - (reply: 1)
  187. analysing protein sequences - How to find domains? (reply: 5)
  188. Gentle extraction of nuclear protein complex - (reply: 2)
  189. Protein into the membrane - (reply: 2)
  190. do i need to desalt my protein after purification - (reply: 1)
  191. DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
  192. reverse phase hplc of proteins.. mechanism of binding and elution.. - your views.. ! (reply: 3)
  193. BSA vs. Nonfat dry milk for serine/threonine phosphorylation sites - which is better for quality blots? (reply: 1)
  194. Western Blot for weakly expressed protein - (reply: 3)
  195. My protein shows 3 bands - Reb1 phosphorylation? (reply: 1)
  196. A ROS producer that doesn't cause protein carbonylation - (reply: 2)
  197. Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
  198. large quantity of high concentration protein needed - (reply: 3)
  199. resolving 27 kDa protein - (reply: 3)
  200. small volume spectrophotometrical protein quantification - (reply: 1)
  201. HELP: protocol for protein extraction - (reply: 4)
  202. Killing bacteria without denaturing proteins - (reply: 9)
  203. immunohistochemistry and protein denaturation - (reply: 3)
  204. How Conserved are the "Constant" regions of 'IgG' Heavy and Li - (reply: 5)
  205. Protein is not binding to the ion-exchange column. - (reply: 4)
  206. programme to predict amphipathic helices in a protein aminoacid sequence - (reply: 1)
  207. How to tackle the problem of protein denaturation while storing? - (reply: 10)
  208. Just a smear of proteins after ponceau red - Western Blot (reply: 4)
  209. Storing tissue for protein and RNA extraction? - (reply: 13)
  210. different western results when using acetone prep vs crude protein - (reply: 3)
  211. Gap43 protein western Blot - (reply: 7)
  212. post-translational modifications which increase molecular weight of the protein - (reply: 7)
  213. SDS Gel - My protein bands go to the bottom of gel (reply: 9)
  214. how to refold insoluble protein after purification - (reply: 10)
  215. 10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
  216. how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
  217. G protein inhibition - (reply: 3)
  218. Isolation of golgi transmembrane protein - (reply: 4)
  219. protein nature in different pH? - (reply: 3)
  220. protein determination in loading buffer - (reply: 1)
  221. oligos phosphorylation with pnk - (reply: 4)
  222. problem in expression of target protein - (reply: 11)
  223. concentrating low expression protein for western - will this work? (reply: 3)
  224. Protein Refolding problem - (reply: 2)
  225. Consistency problems with Micro BCA assay - (reply: 4)
  226. Buffer Exchange & Protein Purification - (reply: 1)
  227. Do I need to do BCA assay to quantify the protein before doing the ELISA? - BCA assay and ELISA (reply: 9)
  228. storage buffer for purified protein - (reply: 3)
  229. new techniques about protein ladder? - (reply: 3)
  230. Solubilisation of proteins out of membranes - Which detergent at what concentration (reply: 1)
  231. marker proteins for plasm membrane in c2c12 myoblasts - (reply: 1)
  232. protein array - (reply: 1)
  233. EMSA for non-DNA binding protein - (reply: 1)
  234. who has done biotin pull down RNA interacting protein before? - biotin pull down RNA interacting protein (reply: 3)
  235. Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
  236. MAJOR PROBLEM IN EXPRESSING GST TAGGED PROTEIN - (reply: 1)
  237. Problems with inconsitant protein recovery using ammonium sulphate (AS) precipit - protein precipitation/concentration (reply: 2)
  238. Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
  239. What is the best protein expression system - (reply: 1)
  240. Big problem in GST Protein purification - (reply: 9)
  241. Protein extraction from animal cells - (reply: 7)
  242. preparing samples for phosphorylation sites identification - (reply: 3)
  243. Trying to get protein out of insoluble fraction - (reply: 3)
  244. getting rid of protein above 60 kDa - (reply: 7)
  245. Suggested tool for protein analysis? - (reply: 4)
  246. What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
  247. Protein G purified IgG>>> extra band at approx 200 kd - (reply: 5)
  248. How to stain protein fiber to view in light microscopy? - (reply: 1)
  249. Protein reversed phase analysis.. linear or step gradients? - which is preferred and why?? (reply: 3)
  250. protein isolation from cell lines - membrane bound proteins (reply: 3)
  251. Protein Extraction - (reply: 1)
  252. Can you get two proteins made from a heterozygous gene mutation? - (reply: 3)
  253. Any good method for resuspension protein from TCA/acetone precip ? - (reply: 4)
  254. SDS-page & lipidated proteins - (reply: 6)
  255. Long PCR primers for epitope tagging - where to order? (reply: 3)
  256. Separation of piperidine from proteins after AIC - (reply: 1)
  257. IgG purification: Multimerization... - (reply: 4)
  258. Protein crashing out of solution - (reply: 4)
  259. Protein expression in E.coli DH5alpha? - (reply: 1)
  260. question about protein molecular weight - (reply: 12)
  261. Ab's production from recombinant proteins (2) - new idea (reply: 2)
  262. how to identify the significance of a protein - (reply: 1)
  263. Ab's production from recombinant proteins - (reply: 4)
  264. how to define an appropriate protein reaction buffer? - (reply: 1)
  265. Probelm with expression of ~85 kDa GSt fusion protein. - (reply: 5)
  266. amount of protein for MALDI-TOF - (reply: 5)
  267. Electro Elution of my protein after SDS-PAGE - (reply: 7)
  268. Western for membrane bound protein - (reply: 11)
  269. Size Exclusion Column - Proteins not coming out ! - BMP-2 (30 kDa) is inhjected in a colum (size exclusion at 100kDa) and (reply: 2)
  270. Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
  271. antibody against yeast protein - (reply: 1)
  272. In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
  273. My fusion protein's reading frame disrupted? - Please help...... (reply: 4)
  274. Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
  275. ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
  276. Epitope tagging - Several basic questions (reply: 2)
  277. Prtocol for large scale protein expression - (reply: 1)
  278. Methods needed for gene/protein identification and function - (reply: 2)
  279. detecting small membrane protein by western blot - (reply: 4)
  280. losing protein expression after stablizing cells( please help) - (reply: 4)
  281. Please help me! I am really tired of this stupid protein purification! - (reply: 8)
  282. wetern for membrane associated protein - (reply: 1)
  283. Protein Sample - How to store it? (reply: 1)
  284. protein marker for 2D-PAGE - (reply: 2)
  285. [In vitro Kinase assay] Do phosphorylated proteins migrate more in SDS-PAGE? - (reply: 3)
  286. Measurement of protein- protein interaction? - (reply: 2)
  287. HPV E7 protein - (reply: 1)
  288. Rabbit/pig/human IgG Light chain amino acid sequence needed - (reply: 1)
  289. IP first-timer - doubts on equal protein concentration control (reply: 2)
  290. no protein expression - (reply: 2)
  291. Protein stability in acetonitrile - storage conditions? - (reply: 1)
  292. Sensitive protein assay - (reply: 2)
  293. timing - DNA to RNA to Protein (reply: 1)
  294. when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
  295. protein band is shifted after incubation with plant protein extracts! - (reply: 2)
  296. passage of cell culture and protein locolization - (reply: 1)
  297. positive IgG in ChIP - (reply: 2)
  298. What is the most effective method of protein precipitation? - (reply: 2)
  299. protein precipitating - (reply: 8)
  300. Dialysis doubt - (reply: 1)
  301. protein estimation of monoclonal antibody - inconsistent values (reply: 2)
  302. WB trouble - no signal of low kd protein - (reply: 5)
  303. what's the difference between the purification of GST-fused protein and His- - (reply: 4)
  304. validating the efficiency of inhibitors of protein kinases A & G - (reply: 3)
  305. HELP estimation of protein in ammonium sulphate purified igG - (reply: 6)
  306. Protein G beads for IP - (reply: 1)
  307. please help: how to isolate nuclear protein from tissue samples - (reply: 1)
  308. lysis buffer for coip with a membrane protein - (reply: 2)
  309. Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
  310. Antibody not binding to protein A/G - Immunoprecipitation (reply: 3)
  311. Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
  312. problem in centrifuge of protein - (reply: 3)
  313. for protein refolding - (reply: 5)
  314. protein kinase C (PKC) inhibitors - (reply: 1)
  315. Size exclusion chromatography for 23 and 20.5KDa? - protein purification (reply: 3)
  316. extract proteins for WB - (reply: 4)
  317. TCA radiacive protein precipitation without lysis - (reply: 1)
  318. How to chose vector and E.coli strain for protein expression - (reply: 2)
  319. Methods for large-scale protein purification - (reply: 5)
  320. Could protein modification block an antibody epitope? - (reply: 4)
  321. How do i organize immunoblotting for 24 proteins from the same sample? - (reply: 4)
  322. how to investigare new protein - (reply: 2)
  323. protein extract questions - (reply: 1)
  324. Yeast protein extract preparation for a quick western blot - (reply: 5)
  325. Loss of LMW in Protein Standard - (reply: 2)
  326. promoter regulation but not protein regulation - (reply: 4)
  327. why express mamallian proteins in DH5alpha ? - (reply: 1)
  328. Protein expression - (reply: 1)
  329. Stability of protein back bone? - (reply: 6)
  330. Problems experienced from adding 3xHA epitope to the C- terminus of some interes - Problems experienced from adding 3xHA epitope to the C- terminus of so (reply: 2)
  331. ELISA-amount of total protein to coat wells - (reply: 2)
  332. Protein extraction-Lysis Buffer - WB (reply: 1)
  333. SOS for GST-fusion protein expression - (reply: 1)
  334. Find a known domain a protein? - (reply: 3)
  335. reuse of Protein G in IP procedure - (reply: 2)
  336. plasmid prep with protein? contamination - (reply: 3)
  337. Temperature effect of protein? - (reply: 5)
  338. Protein loading control? - (reply: 4)
  339. how can I get enough proteins for Mass spec? drive me mad! - (reply: 12)
  340. Identify one protein among several - (reply: 4)
  341. IgG purification - amonium precipitation method (reply: 5)
  342. problem with overexpression - mRNA vs protein level (reply: 2)
  343. 3D protein structure - (reply: 2)
  344. What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
  345. phospho protein - (reply: 11)
  346. IgG molecule - molecular weight (reply: 2)
  347. antibody purification - how to get rid of bovine IgG? (reply: 3)
  348. tips for detect phosphorylated proteins - (reply: 2)
  349. transfected protein sequestered inside vesicles - (reply: 3)
  350. Western Blots with (250 kDa) High Molecular Weight Protein - (reply: 2)
  351. Trouble finding 5kDa protein in western blot - (reply: 5)
  352. Larger sized Protein Standard Ladder Req - (reply: 1)
  353. identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
  354. GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
  355. anti IgM secondary HRP? - (reply: 1)
  356. cell lysis to investigate phosphorylated protein - (reply: 1)
  357. protein ppt removes nucleotides? - (reply: 3)
  358. How do you know if a specif Ser of the substrate protein is phosphorylated by a - (reply: 3)
  359. isolation of protein complex by use percoll gradient - (reply: 1)
  360. siRNA causing no protein knockdown - (reply: 1)
  361. mass spectrometry analysis protein identification drive me crazy - (reply: 9)
  362. How to renature denatured protein? - (reply: 4)
  363. Separation of protein size of 20kDa and 150kDa - (reply: 4)
  364. Can membrane proteins be separated using 2D-PAGE?!?! - (reply: 2)
  365. Help--how to isolate the monomer proteins - (reply: 3)
  366. Protein purification - (reply: 2)
  367. 40kDa protein for ladder - (reply: 4)
  368. loading control for nuclear protein in rabbit cell extract - (reply: 1)
  369. Vector for expressing proteins to be secreted - (reply: 3)
  370. Protein Extraction from cell media for western blot - (reply: 3)
  371. N-terminal protein sequencing.. "holes" at lysine residues.. meaning? - what kinda modifications cud've happnd? (reply: 2)
  372. Western Blot for Higher molecular weight Proteins - (reply: 1)
  373. HELP! contaminants do not separate from my protein during purification - (reply: 5)
  374. Protein Expression Problem - (reply: 5)
  375. How to measure protein expression levels of multiple forms of glycosylated prote - (reply: 2)
  376. Protein from Liver tissue - (reply: 2)
  377. Problem with Protein purification - Protein seems to stick to sepharose (reply: 6)
  378. Estimating Protein Mass - Best way to quantitiate protein (reply: 1)
  379. Phospho protein % in the cell - signal transduction (reply: 1)
  380. Total proteins extraction - (reply: 2)
  381. Epitope mapping - (reply: 5)
  382. Surface proteins isolated by biotinylation are contaminated.. - (reply: 3)
  383. membrane protein western blot - (reply: 3)
  384. where can I access the all known proteins express in brain? - perhapes a database , but where to find it? (reply: 4)
  385. Protein purification - (reply: 2)
  386. Incomplete transfer of proteins for western blot - (reply: 4)
  387. how to avoid cytosolic proteins in nucleus - (reply: 3)
  388. Mammalian protein expresion in bacteria - Which plasmid and strain to use (reply: 7)
  389. pET IPTG expression - Getting protein expression (reply: 1)
  390. PRotein marker can give concentration of my protein in gel? - (reply: 4)
  391. Gel shift with high isoelectric point protein - (reply: 1)
  392. ChIP IgG has the same signal than the antibody tested - (reply: 9)
  393. Protein lysis buffer for mass spec - recipe (reply: 15)
  394. peptide phosphorylation analysis method - (reply: 3)
  395. does low mRNA expression mean low relevant protein expression - looking for answers or references (reply: 9)
  396. Hard to dissolve pelleted protein after TCA precipitation - Urgent!! Please check this out!! (reply: 2)
  397. Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
  398. Question for purchasing purified protein - (reply: 3)
  399. Enough protein for mass spectrometry - How much is enough? (reply: 2)
  400. Protein cleavage site? - (reply: 1)
  401. MW marker for 150 kDa protein in Western blot - (reply: 5)
  402. Correcting phospho-protein with total protein - (reply: 2)
  403. Protein insoluble in 8M urea or 6MGuHCl - (reply: 8)
  404. purification of proteins from sample - (reply: 12)
  405. how can I dialyze protein through its PI and avoid precipitation? - (reply: 1)
  406. questions on protein glycosylation - (reply: 2)
  407. Immunoprecipitation & Mass Spec analysis - How much mg(ug) of protein lysate is needed for IP followed by Mass Sp (reply: 2)
  408. dialysis tubes for chromatin purification - (reply: 5)
  409. Protein expression. Question of V5 epitope and 6x His tag ! - (reply: 2)
  410. EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
  411. protein chromatography - (reply: 5)
  412. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
  413. CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
  414. CFP-protein - fuses proteins (reply: 1)
  415. 2D-PAGE PROTEIN ANALYSIS - SECRETED PROTEIN DESALTING (reply: 4)
  416. Gel Loading- Equal Protein or equal volumes? - (reply: 2)
  417. How to test the relationship between secreted protein and signaling pathway - (reply: 4)
  418. loading control for mitochondrial protein - (reply: 1)
  419. Protein expression Using NEB yest kit - (reply: 1)
  420. GST fusion protein preparation protocol and troubleshooting - (reply: 1)
  421. Protein Solubility - How to determine? (reply: 2)
  422. E coli protein extraction for EMSA - (reply: 1)
  423. Plant Protein Extraction - (reply: 2)
  424. Chemiluminescent protein marker - (reply: 3)
  425. Silver stain Protein detection - Silver stain problem (reply: 4)
  426. Preventing protein aggregation - (reply: 2)
  427. Method to determine whether protein exists as Monomer/Dimer etc.? - (reply: 6)
  428. half life of EGFP protein - (reply: 3)
  429. subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
  430. Transfer optimization of membrane proteins to PVDF - (reply: 3)
  431. protein precipitated - (reply: 2)
  432. Phosphorylated Proteins - (reply: 3)
  433. How to purify protein secreted in medium - (reply: 2)
  434. Protein isolation - Small size His-tag Protein isolation (reply: 4)
  435. Protein purification - (reply: 3)
  436. protein extraction from SDS-PAG (GeBAflex) - your opinion about usefulness (reply: 1)
  437. Why the DNA sequence big different but protein the similarity? - such as MAOA and MAOB genes (reply: 4)
  438. Loss of phosphorylation after gel purification? - (reply: 3)
  439. How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (reply: 2)
  440. Retinoic Acid Receptors Protein Extraction - (reply: 2)
  441. Protein purification - (reply: 3)
  442. protein extraction from muscle biopsies - protocols needed (reply: 3)
  443. Protein high molecular weight standards for gel filtration - (reply: 2)
  444. How many methods can demenstrate to endogenous protein interactio? - (reply: 3)
  445. Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
  446. will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
  447. Protein storage and BSA - (reply: 1)
  448. A CoIP problem - How to reduce the non-specific binding of Protein A beads? (reply: 4)
  449. SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
  450. native protein - (reply: 2)
  451. concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
  452. Serum ELISA IgG titer quantitation - How to determine IgG titer without known sample? (reply: 4)
  453. Does protein A affect Protein B level in my case? - need advice on my confusing result (reply: 7)
  454. Specific and Total Activity for Protein Purification - (reply: 2)
  455. Purified protein has no activity - (reply: 8)
  456. Protein purification after cleavage (thioredoxin) - (reply: 3)
  457. Isolating c. elegans protein free from bacterial's - (reply: 1)
  458. CIP and protein - (reply: 2)
  459. 2D protein gel for miRNA target screening? - (reply: 2)
  460. confusing shape of survivin protein band - (reply: 3)
  461. protein concentration - bradford method (reply: 9)
  462. size of tagged proteins - (reply: 3)
  463. Which water is better for chemical preparation ? - water grade for protein purification (reply: 2)
  464. Fusion protein without stop codon? - (reply: 1)
  465. large protein degradation - during baculovirus purification (reply: 2)
  466. truncated protein under normal conditions. - it is possible? (reply: 6)
  467. how to recover my protein? - A terrible mistake (reply: 9)
  468. Quantifying Proteins, help required! - (reply: 4)
  469. His-tag protein expression problem - (reply: 4)
  470. Protein A agarose for CHIP--pre-treatment is needed? - (reply: 2)
  471. How do I prepare a sample to quantify a certain protein with ELISA? - (reply: 10)
  472. specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
  473. Immunostaining of cytoplasmic proteins in nucleus, why? - (reply: 10)
  474. protein degradation - (reply: 2)
  475. Recombinant IL-2 proteins.. - Work for cancer.. how? (reply: 1)
  476. transfer gene to protein? - (reply: 3)
  477. COS-7 and PC-3 transfected with a GFP-AR fusion protein - (reply: 4)
  478. REFOLDING - visualisation of protein after denaturation step? - (reply: 1)
  479. protein extraction from blood sample - (reply: 2)
  480. Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
  481. Expression in codon plus of GST fusion protein - (reply: 3)
  482. Unreliable Protein Induction - (reply: 1)
  483. 2 questions in 1....about transferring large proteins...and blotting - (reply: 7)
  484. Question about phosphorylation and ligation - (reply: 1)
  485. Purification after oligos phosphorylation? - (reply: 1)
  486. Questions about protein gel loading and immunoprecipitation - (reply: 4)
  487. Formaldehyde Cross-Linking for protein - (reply: 1)
  488. How to get rid of nuclease in protein preparation? - (reply: 5)
  489. Why BSA can stabilize proteins? - (reply: 8)
  490. EMSA with recombinant protein - (reply: 2)
  491. CBB DYE BOUND TO PROTEIN - (reply: 2)
  492. his-tag protein expression - inoculation of large cultures with plate colonies/ starter cultures vs. glycerol (reply: 1)
  493. Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
  494. protein precipitation upon dialysis - (reply: 5)
  495. ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
  496. faster proteins - (reply: 1)
  497. Big protein - (reply: 6)
  498. protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
  499. in vitro translation - how is the protein purified? (reply: 12)
  500. mobility of protein band over PAGE - please suggest (reply: 1)
  501. getting rid of contaminant proteins in a Ni column using imidazole - (reply: 2)
  502. PHOSPHORYLATION - (reply: 15)
  503. precipitation of nuclear proteins during GST pull down assay - (reply: 1)
  504. Protein concentration measure with NanoDrop - (reply: 22)
  505. Protein extraction from soya meal using membrane - (reply: 1)
  506. Strange: mRNA expression goes up but protein goes down - (reply: 2)
  507. Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
  508. Purified Protein Aggregation/Multimerization Problem - (reply: 3)
  509. Protein analysis - (reply: 4)
  510. Protein purification before mass-spec - need sample purification methods (reply: 4)
  511. Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
  512. what these components do in the buffer used in protein purification - (reply: 5)
  513. protein folding- looking for renaturation buffer - (reply: 1)
  514. Ultrasonic homogenizer for protein and enzyme extraction? - (reply: 3)
  515. Low protein yield with immunoprecipitation - (reply: 3)
  516. heating of samples during plant protein extraction - (reply: 2)
  517. Protein extraction from SDS gel - (reply: 6)
  518. Protocol for protein isolation from culture media - (reply: 3)
  519. detecting transmembrane protein exoression for monoclonal ab production - (reply: 1)
  520. acetone for protein precipitation - grade of purity (reply: 3)
  521. SDS-PAGE protein aggregation and precipitation - (reply: 1)
  522. Protein extraction/lysis from whole tissue - Do you have a protocol? (reply: 3)
  523. Is ChIP is a good choice to find the DNA sequence for a binding protein? - (reply: 2)
  524. too much protein for Western? - (reply: 5)
  525. Low molecular weight protein problem - (reply: 11)
  526. Binding partners to protein of interest - (reply: 1)
  527. specific protein in high quantities - what can I do? (reply: 3)
  528. How much can I believe protein MW standards? - (reply: 10)
  529. Does SDS loading buffer + heat completely denature protein? - (reply: 3)
  530. Fusion protein expressing problem - (reply: 2)
  531. What is the maximum amount of protein that could be loaded in WB? - (reply: 2)
  532. G protein coupled receptor - western blot - (reply: 1)
  533. I want to strip the protein off PVDF membrane - (reply: 4)
  534. GFP labelled protein purification - (reply: 1)
  535. Periplasmic protein extraction problem! - (reply: 2)
  536. Protein sample preparation for SDS-PAGE - (reply: 9)
  537. Epitope tag positioning - (reply: 3)
  538. Protein expression - decreased expression (reply: 2)
  539. western - cells treated w/ protein synethesis inhibitor - (reply: 1)
  540. microgram to micromolar conversion - Protein concentration - microgram to micromolar conversion - Protein concentration (reply: 4)
  541. How to normalize the protein expression amongst different tissues - (reply: 7)
  542. cancer-specific proteins - (reply: 1)
  543. Maltose Binding Fusion Protein MBP - which strain for expression? (reply: 1)
  544. can we purify the protein from westernblot membrane? - (reply: 5)
  545. Total protein in human serum - (reply: 2)
  546. PROTEIN AND DNA GEL ELECTROPHORESIS - GEL ELECTROPHORESIS (reply: 4)
  547. amount of proteins isolated from cells - (reply: 2)
  548. Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
  549. protein preparation for Western Blot - (reply: 6)
  550. Medium for adherent cells and serum/protein free - (reply: 2)
  551. protein concentration measurement - (reply: 12)
  552. Secreted protein degraded in conditioned medium? - (reply: 1)
  553. How can I express my protein successfully? - (reply: 10)
  554. how can i use SDS and Triton X-100 to isolate the membrane protein? - (reply: 6)
  555. differential electrophoretic migration of phosphorylated proteins - (reply: 2)
  556. can protein A agarose precipitate anti goat IgG? - (reply: 2)
  557. Maltose Binding Protein - Information on maltose binding protein... (reply: 5)
  558. protein ladder detection for western blotting - (reply: 2)
  559. Detecting phosphorylated PKR - antibody and positive control - (reply: 4)
  560. Selection of best recombinant protein produing strain - selection of industrial strain (reply: 3)
  561. May a CARRIER-antibody compete between them for binding to protein G-agarose? - Immunoprecipitation problem (reply: 10)
  562. cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
  563. NF-kB EMSA - Different nuclear protein extraction method leads to opposite results (reply: 1)
  564. thrombin cleavage and triton? - protein purification (reply: 2)
  565. protein extraction - protein extraction from wheat seed (reply: 4)
  566. How do you purify membrane protein? - help (reply: 2)
  567. Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
  568. Protein level in fibroblasts cell line - (reply: 1)
  569. two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
  570. protein precipitation - (reply: 4)
  571. Protein immobilization on nitrocellulose membrane - (reply: 4)
  572. protein insoluble in just about everything! - (reply: 3)
  573. Protein in active form - (reply: 2)
  574. SDS-PAGE - CAN I USED 12% GEL FOR WHEAT LEAVE PROTEIN SDS-PAGE (reply: 1)
  575. Small fragment RNA isolation from interacting protein - (reply: 1)
  576. MBP fusion protein... - (reply: 6)
  577. Protein formed ppt after stored at -80oC - (reply: 3)
  578. to check protein expression - (reply: 4)
  579. Time point for reduction of protein levels after RNAi - (reply: 1)
  580. quanitation of protein extracted with trizol - (reply: 7)
  581. unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
  582. trouble getting cysteine labeled with biotin-maleimide - cysteine scanning purified protein (reply: 1)
  583. how to determine whether protein level increase is resulted from mRNA stability - (reply: 1)
  584. Protein TCA Precipitation Resolubilization Question - (reply: 3)
  585. Biotinilated proteins. Problems with Hens buffer - (reply: 2)
  586. about protein domains in pfam - (reply: 4)
  587. problem of protein expression - problem of protein expression (reply: 1)
  588. Protein Transfection by Electroporation - (reply: 6)
  589. sds-page of hemocyte lysate proteins - (reply: 2)
  590. Protein expression troubleshooting - problem in expression of my protein (reply: 3)
  591. Question about the strategy of protein purification - (reply: 4)
  592. protein dialysis - (reply: 1)
  593. Why should add DTT and what kind protein assay I should use - (reply: 4)
  594. One or two bands with different protein extraction buffer - (reply: 3)
  595. Membrane protein extraction - What about MEM-PER kit (Pierce)? (reply: 1)
  596. Protein Structure - (reply: 4)
  597. Protein forced aggregation - conditions? (reply: 1)
  598. A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
  599. Protein crystals - (reply: 3)
  600. Overexpression of 630 aa protein - (reply: 3)
  601. IP from frozen protein extracts - (reply: 10)
  602. How to confirm a protein is a receptor? - (reply: 6)
  603. Concentrate protein from dilute solution - (reply: 2)
  604. Protein Precipitation (wessel and Fluege method) - 2 questions (reply: 7)
  605. Cleaving a protein from GST - Troubleshooting methods to liberate my protein from gluthathonine sepharose (reply: 3)
  606. protein purification -- 2 or more columns - (reply: 9)
  607. protein folding - GST-fusion protein fluorescence (reply: 2)
  608. what buffer will prevent the precipitation of protein at -20? - (reply: 5)
  609. what is the best method to concentrate protein - (reply: 2)
  610. protein for crystalization - (reply: 1)
  611. How to convert the OD of protein to its concentration? - (reply: 2)
  612. Protein structure comparison- sortware needed - (reply: 2)
  613. problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
  614. Develop a way to retrieve frequent protein segments by suitable algorithm - program, idea and so on . (reply: 1)
  615. how to remove media components from protein solutions - (reply: 4)
  616. Expression of fusion protein in mammalian cells - (reply: 2)
  617. Membrane protein extraction and detection - General lab tech (reply: 1)
  618. How to elute more protein from flag M2 beads(sigma)? - in hurry (reply: 6)
  619. Cleavage of target protein from glutathione sepharose - (reply: 3)
  620. After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
  621. protein protein interaction - (reply: 3)
  622. Perl script - parsing non-phosphorylated protein sequences from a Uniprot flat f - (reply: 12)
  623. How does protein precipitate? - (reply: 1)
  624. biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
  625. Proportion of folding proteins among all 100 AA long proteins - (reply: 1)
  626. detection of a secreted recombinant protein - (reply: 2)
  627. IEF for pI 9.4 protein - ? (reply: 1)
  628. general protein confimation in lysis buffer - effect of reducing agents (reply: 5)
  629. protein precipitation during concentration - (reply: 5)
  630. PEG to seperate Plasmide from Protein? - (reply: 2)
  631. making large proteins - (reply: 6)
  632. buffer for His-tag recombinant protein purification - (reply: 2)
  633. Losing Low Molecular Weight Proteins in SDS-PAGE - (reply: 2)
  634. Protein from blood samples - (reply: 5)
  635. Aggregated proteins in SDS-PAGE - (reply: 5)
  636. recommendation on mitochondria matrix protein antibody? - (reply: 1)
  637. SDS protein sample over boiled, what happend? - (reply: 1)
  638. how to purify fusion proteins on a large scale? - (reply: 2)
  639. separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
  640. how can I increase protein expression level - hurry (reply: 2)
  641. concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
  642. BCA protein assay - (reply: 3)
  643. Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
  644. how to do western with 500 kD protein? - (reply: 5)
  645. stable or transient transfection method? - for protein expression (reply: 4)
  646. Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
  647. Transfer high molecular weight protein - (reply: 4)
  648. his-tag protein - (reply: 7)
  649. Determining if a protein is secreted - (reply: 12)
  650. Protein denaturation and renaturation - (reply: 2)
  651. His Tag protein purification - (reply: 3)
  652. Protein deglycosylation - (reply: 1)
  653. How to separate protein from nucleic acids? - (reply: 5)
  654. Storing proteins - (reply: 2)
  655. prediction of protein phosphorylation sites, how? - (reply: 4)
  656. problem with the size of protein - (reply: 4)
  657. Western after Ip- no IP protein, but .... - (reply: 1)
  658. immunoprecipitation of nuclear protein - (reply: 2)
  659. Cell line to be used for protein expression an purification - (reply: 8)
  660. Protein expression problem - coexpression? (reply: 1)
  661. Human IgA column - (reply: 1)
  662. His tag protein purification problem - Binding of undesired protein (reply: 6)
  663. protein diffusion in sds page gel - (reply: 2)
  664. Protein disappeared after TCA precipitation - (reply: 7)
  665. Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
  666. Help:Total protein extraction from soil - (reply: 2)
  667. low molecular weight proteins on SDS-PAGE - (reply: 1)
  668. Using ECF for protein detection? - ECF instead of ECL? (reply: 1)
  669. phosphorylated protein sequences with <20% homology - (reply: 5)
  670. Big size proteins in SDS-PAGE - (reply: 3)
  671. anyone working in protein aggregation..? - (reply: 6)
  672. extracellular domain from a transmembrane protein, secreted or not? - (reply: 2)
  673. How to make an antibody against my protein - (reply: 4)
  674. eluting protein from the gel band... - (reply: 7)
  675. transgenic mouse analysis - I have generated a transgenic mouse for a protein that is expressed in the brain (reply: 2)
  676. Protein that doesn't express - (reply: 2)
  677. Problems with protein extraction with Trizol - (reply: 1)
  678. What is the name of substances that covalently binds proteins - (reply: 1)
  679. protein/enzyme activity asssay - (reply: 1)
  680. Protein Half-life determination - (reply: 1)
  681. problems with expression of mutant protein - (reply: 1)
  682. TCA vs. meth/chloro - concentration of proteins (reply: 3)
  683. Protein migration in SDS gel - why? (reply: 2)
  684. crosslink protein complex in vivo - (reply: 1)
  685. problems whit protein solubility after purification - (reply: 5)
  686. What can cause a change in protein level? - (reply: 1)
  687. protein concentration - (reply: 1)
  688. protein complex purification, what would affect? - (reply: 3)
  689. protein extraction from tissue - (reply: 1)
  690. protein isolation from brain material - yield? (reply: 8)
  691. Which kind of post-translation modification can make protein size much bigger (3 - (reply: 8)
  692. problems in concentration protein solution - (reply: 8)
  693. Help! Protein extract degradation ? - (reply: 1)
  694. what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
  695. Concentration of secreted protein - ...step before IP.... (reply: 1)
  696. Protein determination in a insoluble pellet - (reply: 6)
  697. protein contamination in DNA electrophoresis - (reply: 2)
  698. Quick help on determining protein size - (reply: 3)
  699. Problem in protein preparation in 2-D electrophoresis - (reply: 1)
  700. Stabilization of protein for injection in mice - when storing protein in freezer (reply: 2)
  701. How to Draw a 3D structure of a Protein from its amino acid sequece? - (reply: 4)
  702. Anybody that has used Microcon from Millipore to concentrate protein!? - (reply: 8)
  703. protein purification - (reply: 5)
  704. How to calculate protein size in Kilodaltons - (reply: 6)
  705. problem with bacteriocin protein purification - (reply: 2)
  706. protein precipitation / RNA binding factors - (reply: 3)
  707. protein dialysis - i think i just cant understand it (reply: 15)
  708. at which step can I store the protein? - purification from inclusion body... (reply: 3)
  709. Phosphorylated proteins - (reply: 1)
  710. How can I remove the Polyhistindine tag or myc epitope from my recombinant prote - (reply: 3)
  711. RNA binding protein isolation - how to figure out what binds your mRNA? (reply: 1)
  712. cloning membrane protein with 2 tags - (reply: 2)
  713. purification problem. - most of my protein is washed away.... (reply: 10)
  714. protein expression - (reply: 3)
  715. why dose imidazole can elute protein from Ni-column - (reply: 3)
  716. Outer membrane protein in the supernatant!? - (reply: 1)
  717. WHat's the difference between Protein A and G? - (reply: 2)
  718. Denature proteins on the membrane - (reply: 4)
  719. loading protein onto SDS-PAGE gel - (reply: 2)
  720. Protein precipitation using Methanol/Chloroform - (reply: 1)
  721. Protein yeast extraction for Western blot - (reply: 3)
  722. Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
  723. Dialysing the proteins - (reply: 3)
  724. which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
  725. western protein ladder - (reply: 9)
  726. How much Antibody did I get? - Protein G column (reply: 1)
  727. suggest protein purification methods? - (reply: 2)
  728. phosphorylation analysis - (reply: 2)
  729. Please recommend a fast way to get purified protein - (reply: 2)
  730. hesitation between Rasmol and protein explorer - software (reply: 2)
  731. DNase I......how much to use in units? - protein purification issues (reply: 1)
  732. protein extraction from stable mammalian clones - (reply: 8)
  733. Stains for proteins.. - Silver v/s Coomassie (reply: 1)
  734. Need help in Using BLAST for beginner - finding the paralog, ortholog and protein (reply: 1)
  735. protein purification - (reply: 1)
  736. Membrane protein purification - (reply: 1)
  737. question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
  738. native gel -- protein migration - (reply: 1)
  739. Measure OD600 when perform the protein expression - (reply: 6)
  740. Differences Between Hormones - difference between the action of protein and steroid hormones (reply: 1)
  741. Smearing of protein samples from whole tissue lysates - (reply: 6)
  742. Protein Concentration Methods - (reply: 7)
  743. Protein transfer, high resistance causing loss in voltage? - (reply: 2)
  744. Do repeated freeze-thaw cycles affect protein activity? - (reply: 4)
  745. How to analysis glycated proteins on SDS-PAGE - (reply: 1)
  746. General equation for protein degradation - (reply: 1)
  747. His-tag fusion protein detection problem - (reply: 2)
  748. Separating DNA and nucleotides from protein - (reply: 4)
  749. many bands other than my protein on western... - please help me to explain this... (reply: 5)
  750. protein precipitation for mouse liver - (reply: 1)
  751. bee venom peptides, proteins, enzymes - (reply: 1)
  752. Protein elution question - (reply: 1)
  753. Protein purification from E Coli - (reply: 11)
  754. membrane protein western blot - (reply: 2)
  755. Western/Signaling Studies - How to get rid of background phosphorylation? - Western/Signaling Studies - How to get rid of background phosphorylation? (reply: 3)
  756. big black spot in the place of my protein on western... - (reply: 4)
  757. PVDF versus nitrocellulose - which is better for simple Western? - "normal" protein of 13kDa (reply: 4)
  758. Protein degradation - (reply: 1)
  759. extract protein from SDS PAGE - (reply: 1)
  760. detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
  761. Nuclear protein in a post nuclear supernatant? - (reply: 6)
  762. How to stop protein from preciptating during dialysis - (reply: 4)
  763. Phenol chloroform extraction DNA: protein contamination - how to remove all protein from my DNA? (reply: 1)
  764. Liver tissue lysis for ER protein Western blotting - (reply: 2)
  765. Calcium homeostasis and protein - relationship between calcium homeostasis and some proteins? (reply: 3)
  766. 38kda protein appearing as smear on western - (reply: 5)
  767. sds page of protein in tfa & acotonitrile - (reply: 1)
  768. protein glycosylation analysis - (reply: 3)
  769. Protein purification - (reply: 3)
  770. Looking for genomic targets of a DNA binding protein. - (reply: 1)
  771. Imidazole in column elution - Removing Imadazole from purified protein without dialysis (reply: 1)
  772. How to get DNA binding protein in the clear supernatants? - (reply: 8)
  773. Expected protein size ? - fusion protein-size in kDa? (reply: 8)
  774. problem transfering small proteins - (reply: 4)
  775. any idea about running small size of protein? - (reply: 6)
  776. DNA amount for protein expression - protein His-tag (reply: 1)
  777. Knockdown protein expression using rnai - (reply: 4)
  778. SIZE OF PHOSPHORYLATED PROTEINS - (reply: 6)
  779. Blotting time and voltage for a 20kd protein - (reply: 4)
  780. unwanted protein degradation - question (reply: 1)
  781. How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
  782. protein loss in sterile filtration - suggest the 0.22 micron filter (reply: 4)
  783. isolation of protein from blood - isolation of protein from blood (reply: 1)
  784. Porblem using streptavidin coated beads - Using streptavidin coated beads to purify proteins tagged with a biotinylated ol (reply: 2)
  785. protein expression in mammalian cell - (reply: 1)
  786. How to seperate protein and carbohydrate from a mixture - (reply: 6)
  787. [32] phosphorylation hints needed! - (reply: 1)
  788. protein loss during nickle clolumn purification - is there always a lot loss of protein during column purification? (reply: 2)
  789. Shipment of extracted proteins - (reply: 3)
  790. membrane protein extraction from dissected thymus - (reply: 1)
  791. How to calculate the size of a protein (in kDa)? - (reply: 1)
  792. Protein extraction - (reply: 1)
  793. protein ppt using acetone - (reply: 1)
  794. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
  795. What is the best way to identify a protein isoform? - (reply: 4)
  796. need ideas for protein protein interaction - protein interaction assays (reply: 3)
  797. way to confirm that my protein is active - (reply: 4)
  798. Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
  799. protein synthesis - (reply: 2)
  800. How amido black stains protein? - (reply: 1)
  801. GST protein purification - only 3% bind to beads (reply: 6)
  802. Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
  803. why get rid of imidazole in his tag protein purification - (reply: 3)
  804. Protein expresses wrong size - (reply: 5)
  805. What is the best nuclear protein marker? - Fractionation (reply: 3)
  806. renilla Luciferase and protein concentration - (reply: 1)
  807. Protein sequence comparison report - (reply: 2)
  808. Help RNA and protein extraction - i dont understand the chemistry behind it (reply: 3)
  809. his tag protein not expressing - I am stuck !!! (reply: 6)
  810. 6 His tagged protein purification - (reply: 1)
  811. PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
  812. Trouble expressing a protein in transient transfection - (reply: 2)
  813. Problem resuspending precipitated protein - (reply: 2)
  814. How to prevent the protein degradation - protein degradation (reply: 2)
  815. loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
  816. trouble in scaling-up protein expression - (reply: 5)
  817. How to measure labelled protein concentration? - (reply: 1)
  818. His-tag protein purification - (reply: 1)
  819. Human IgG from protein G - (reply: 1)
  820. Same protein, different size on Western and IP ? - (reply: 2)
  821. imidazole in protein purification - (reply: 2)
  822. Western transfer time: Is it crucial for big proteins? - anomaly HELP (reply: 2)
  823. protein dephosphorylation by alkaline phosphatase? - (reply: 8)
  824. problem in expression of his tag protein - (reply: 6)
  825. Strange protein transfer - (reply: 3)
  826. IP the membrane protein - How to elute the protein complex from the beads (reply: 2)
  827. anti-c-myc-agarose protein purification - (reply: 1)
  828. Phosphorylation with PNK - (reply: 5)
  829. pure protein - .. (reply: 4)
  830. protein extraction from leaf - (reply: 4)
  831. Quantify protein expression - (reply: 1)
  832. which tag and vector is the best for expressing protein in bacteria? - (reply: 4)
  833. CHIP with Tagged Proteins - (reply: 2)
  834. question about natively seperation of subunits of complex protein by PAGE - (reply: 1)
  835. How to quantify fluorescent-tagged protein - (reply: 2)
  836. help - small proteins and western blotting - (reply: 23)
  837. protein extraction efficiency - protein extraction efficiency (reply: 3)
  838. Loss of proteins during Western transfer - (reply: 4)
  839. Aggregation of protein after purification? - (reply: 2)
  840. protein isolation of plant tissue -trizol regent - (reply: 3)
  841. gene expression vs protein expression - (reply: 3)
  842. Protein analysis of conditioned media - How to prepare media before SDS-PAGE? (reply: 4)
  843. Phosphorylation - (reply: 8)
  844. How to detect receptor on the cell surface? - using the less viral protein possible (reply: 1)
  845. codon bias? - protein expression (reply: 11)
  846. Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
  847. Protein rofolding problem - percipitation (reply: 4)
  848. sonication free protein extraction... - (reply: 2)
  849. Effect of APS (Ammonium persulfate) to protein - (reply: 1)
  850. protein did not enter the gel on EMSA - (reply: 2)
  851. 6 his tagged protein purification - purification (reply: 2)
  852. ELISA with denaturated proteins? - (reply: 6)
  853. Expressing GST-Fusion protein - Are larger protein inserts harder to express? (reply: 4)
  854. Protein samples still in liquid form at -20C - Storage condition for proteins (reply: 8)
  855. protein purification - (reply: 3)
  856. Does imidazole absorbe at 280 nm? - How do you measure your protein content? (reply: 4)
  857. the range of protein amount for loading - western blotting (reply: 4)
  858. optimal SDS separation protein - (reply: 3)
  859. phosphorylated oligos and vector - (reply: 2)
  860. Protein Structure Prediction - HELP!! (reply: 4)
  861. PQE vector - Getting protein in its soluble form (reply: 3)
  862. sample preparation for IEF - trouble in normalizing protein (reply: 1)
  863. Purifying a membrane protein - - the trouble with lipids? (reply: 3)
  864. determining protein expression level from a Western!!! - (reply: 6)
  865. Help with concentrating proteins in culture medium - (reply: 4)
  866. WB- proteins of lower-than-expected MW - (reply: 1)
  867. Making solutions for protein prep - (reply: 3)
  868. shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
  869. protein sizes - which tools (reply: 1)
  870. How much a protein converted into a crystal? - crystallization (reply: 2)
  871. How to make pre-stained protein marker in lab - (reply: 1)
  872. How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein (reply: 3)
  873. Yeast Fluorescent Proteins - Yeast Fluorescent Proteins (reply: 1)
  874. What is the role of integral membrane proteins in signaling? - (reply: 1)
  875. brain protein isolation for WIB - can I simply use 4xLB+BME? (reply: 2)
  876. GFP protein not soluble? - (reply: 1)
  877. Protein hydrophobicity - (reply: 1)
  878. Expressing protein in a soluble form - What should be the next logical step? (reply: 8)
  879. Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
  880. MBP tagged protein not bind resin - (reply: 8)
  881. cDNA size VS protein Size - MW weight confusion (reply: 7)
  882. Acetyl coenzyme A and dialysis - Removing AcCoA and dialysis problem (reply: 2)
  883. Protein Expression - Question (reply: 1)
  884. aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
  885. Protein interaction - (reply: 3)
  886. Stability of protein in powdered leaf sample - Protein stability after quick freeze in liquid N2 (reply: 1)
  887. Protein not penetrating the resolving part of the gel - (reply: 2)
  888. Does SUMO fusion protein increase solubility? - (reply: 2)
  889. <10kD protein in Western - (reply: 3)
  890. Mitochondrial, cytosolic and Nuclear Protein extractions - An all in one protocol? (reply: 1)
  891. whether someone know about such a kind of proteins - (reply: 1)
  892. Proteins and marker are not running down gel - Protein purification (reply: 7)
  893. prepartion of membrane protein - (reply: 3)
  894. About pH and pI for his-tagged protein purification - (reply: 2)
  895. Homemade DNA/Protein A beads - i think i made a boo-boo (reply: 2)
  896. Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
  897. Methods of proteins extraction from plants - protein extraction industry (reply: 1)
  898. glycerol is still there after dialysis - (reply: 1)
  899. Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
  900. Protein isolation for western - (reply: 8)
  901. PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
  902. Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
  903. Can antibodies and proteins be vortexed? - (reply: 4)
  904. aggregates in SDS-PAGE? - double mw of proteins in SDS-page (reply: 2)
  905. in-vivo method of identifying 2 protein binding? - (reply: 3)
  906. quantify protein on beads? - (reply: 4)
  907. "Help" : how to identify of proteins binds to specific DNA - (reply: 3)
  908. Proposal for strategy to determine 2 protein interactions? - suggest sugges! (reply: 11)
  909. western of a big protein - (reply: 2)
  910. Insect cell sonication protocol? - Protein Purification (reply: 1)
  911. Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
  912. How to solve a protein when not soluble in 6M GuHCl? - (reply: 1)
  913. GST proteins detection - (reply: 1)
  914. A question about cell stain: fix the cell have fusion protein - (reply: 1)
  915. Help on protein integrity Western Blot - Sample preparation and detection (reply: 6)
  916. how quick the protein get degraded in room temp - (reply: 6)
  917. DNA "clean-up" - How to get rid of DNA contaminants: RNA, proteins (reply: 1)
  918. STAT 1 / phospho STAT 1 - can't detect my protein (reply: 3)
  919. Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
  920. Protein sticking to membrane - Protein concentration woes (reply: 1)
  921. His-tagged protein binding problem - Protein Binding (reply: 1)
  922. a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
  923. problem with DNA extraction - can't get rid of protein phase (reply: 3)
  924. BCA assay - (reply: 2)
  925. Protein consensus pattern (motif) database - (reply: 1)
  926. IgG's "epsilon" - (reply: 2)
  927. protein quantification- triton - (reply: 3)
  928. phosphorylation - (reply: 5)
  929. what generally methods to improve the protein solubility - (reply: 1)
  930. solubility of protein - (reply: 1)
  931. NP40 concentration in protein lysis buffer - (reply: 1)
  932. help to see whether my protein is imported to the nucleus - please give some advice (reply: 5)
  933. protein quantitation - for equal western loading (reply: 2)
  934. Protein Homogenization - Soluble and insoluble protein (reply: 3)
  935. Protein produced with brown color? - I have some problem in protein expression (reply: 1)
  936. His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
  937. recombinant protein lost immunological activity - (reply: 1)
  938. more questions about protein expression... - (reply: 1)
  939. Express a vaccine protein - (reply: 1)
  940. Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
  941. Protein with His6 tag - (reply: 2)
  942. Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
  943. Protein degradation - Recombinant protein degradation in insect cells (reply: 1)
  944. isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (reply: 1)
  945. Removing Exonuclease From Protein Prep - How can I remove exonuclease from a protein prep (reply: 4)
  946. Cleaved GST fusion protein retained on beads! - Affinity of GST-cleaved protein for glutathione sepharose (reply: 7)
  947. the protein band disappear in the second dimension - 2D-Gel (reply: 2)
  948. antibodies to epitope tags - (reply: 1)
  949. protein precipitation after IMAC - protein purification (reply: 1)
  950. measuring protein concentration - (reply: 5)
  951. Solubility ribosomal proteins - Solubility ribosomal proteins (reply: 4)
  952. Protein extraction from rat liver tissue - (reply: 1)
  953. Protein purification - Problem with his-tag protein gel filtration (reply: 5)
  954. Have any one know about Protein A - (reply: 2)
  955. neomycine resistance protein antibody - does anyone exist? (reply: 2)
  956. Protein expression problems - (reply: 4)
  957. Bacterial Periplasmic protein localization - B-lactamase- alkaline phosp Fusion experiments (reply: 4)
  958. quesiton about GST fusion protein - (reply: 1)
  959. How to predict the 3-D structure with the sequence of protein? - (reply: 4)
  960. Removal of endotoxin from proteins & DNA - Protein & DNA purification (reply: 15)
  961. problem with expression of GST fusion protein - (reply: 1)
  962. protein purifiacation - (reply: 1)
  963. trizol extraction for phospho proteins - (reply: 4)
  964. aggregation of purified proteins - (reply: 4)
  965. How imidazole can be washed off the Ni-NTA agarose beads - reuse of Ni-NTA agarose beads for His protein (reply: 2)
  966. total protein in Western blotting - (reply: 1)
  967. Problem in protein expression - (reply: 5)
  968. Protein reduction without mRNA reduction? - (reply: 1)
  969. UV (280nm) Protein Quantification - Rough estimation of [protein] does it work? (reply: 1)
  970. let me have a simple test for detecting phosphoproteins - (reply: 5)
  971. Binding of protein to immunoassay plates - binding in denaturing conditions (reply: 1)
  972. Protein solubilization and Western Blotting - (reply: 1)
  973. check protein expression from tissue - (reply: 1)
  974. Protein Structure - (reply: 1)
  975. Phosphorylation not affecting SDS-PAGE mobility - (reply: 7)
  976. remove BSA from IgG prep - (reply: 2)
  977. losing protein activity - (reply: 3)
  978. Extraction of membrane proteins and western on them - (reply: 3)
  979. I forgot to pretreat my dialysis membrane ! - (reply: 1)
  980. Who is my closest gene relative? - Help to determine phylogeny of a protein (reply: 2)
  981. protein purification - his-tag protein purification (reply: 2)
  982. choosing insect cell for high-cystine protein expression - (reply: 1)
  983. SDS-PAGE question - seperate protein (reply: 3)
  984. help with protein size - western blot (reply: 1)
  985. protocols for mouse cells - splenocyte preps, protein extraction (reply: 1)
  986. RNA/protein Isolation - Is there some good KIT or ...? (reply: 3)
  987. problems with loss of purified protein due to precipitation - protein purification (reply: 4)
  988. Phosphorylation of protein - Trouble with backphospho (reply: 3)
  989. protein lysates preparation - (reply: 4)
  990. Protein expression troubleshooting - (reply: 2)
  991. protein expression - How to express protein of a high molecul (reply: 4)
  992. 293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
  993. question about SDS-PAGE - electrophoresis of proteins (reply: 4)
  994. Purification of nuclear proteins - (reply: 2)
  995. TCA precipitation of proteins - (reply: 15)
  996. western blotting of expressed protein - (reply: 1)
  997. protein expression in Pichia pastoris - (reply: 91)
  998. help with protein expression and detection - (reply: 4)
  999. ionic Charge and pI of protein information!? - Proteomic (reply: 2)
  1000. isolate secreted proteins - (reply: 3)
  1001. Protein concentrators - (reply: 1)
  1002. DNA binding proteins - (reply: 3)
  1003. Dot blot on bacteria - screen of protein expression (reply: 1)
  1004. western blot for membrane protein with his-tag - can't detect with anti-his tag Ab? (reply: 3)
  1005. FORTE protein profile¨Cprofile comparison tool - (reply: 1)
  1006. Extracting Protein Using TriZOL - Is it possible? Are there problems? (reply: 3)
  1007. Triton X-100 - solubilization of proteins (reply: 1)
  1008. Protein Expression - (reply: 1)
  1009. concentrate protein in culture media? - (reply: 2)
  1010. Detection of low abundant proteins with IHC - (reply: 1)
  1011. Protein pellet solubilisation - (reply: 4)
  1012. His purification of a regular expressed protein - (reply: 1)
  1013. protein precipitation - protein precipitation (reply: 5)
  1014. Spec problems - Trying to measure protein conc. (reply: 2)
  1015. tissus proteins extraction - (reply: 3)
  1016. Hydrophobic proteins extraction from yeast wall cell - How to extract hydrophobic proteins? (reply: 1)
  1017. Protein extraction protocol - (reply: 1)
  1018. Protein assay - Percoll containing samples (reply: 1)
  1019. total protein assay in cell extracts - (reply: 3)
  1020. Yeast protein extraction - need to change my expression system... (reply: 3)
  1021. Precipitation of Native Protein - IMAC of Native Protein (reply: 1)
  1022. Autoradiography for proteins - Radioactive protein probes (reply: 1)
  1023. protein precipitates after dialysis - Protein purification (reply: 4)
  1024. PROTEIN ASSAY - LISTING ALL THE PROTOCOLS FOR ASSAYING IMPORTANT PROTEINS (reply: 2)
  1025. PROTEIN ASSAY - MEASUREMENT OF PROTEIN IN CELL LYSATES (reply: 2)
  1026. How to extract hydrophobic proteins from yeast wall cells? - (reply: 1)
  1027. ELISA - direct protein assay with ELISA (reply: 1)
  1028. Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
  1029. electroelution of protein fromsds-page for use in immunization - (reply: 2)
  1030. IgM Purification - (reply: 1)
  1031. IgM hypermutation - (reply: 1)
  1032. about IgG - (reply: 1)
  1033. Protein extraction from humantissue - (reply: 1)
  1034. problem with trizol - solubilization and western of proteins (reply: 2)
  1035. Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
  1036. his-tagged protein purification - (reply: 1)
  1037. protein express - (reply: 1)
  1038. Sigma nuclear protein extraction - (reply: 1)
  1039. How to concentrate protein from culture medium - for Western blot (reply: 1)
  1040. Plant Protein Extraction - (reply: 1)
  1041. Flag Tagged Proteins - (reply: 1)
  1042. Polysaccharides co-precipitatingwith protein in ammonium sulfate - (reply: 1)
  1043. Protein extraction form gels - (reply: 2)
  1044. electroelution - precipitate on dialysis (reply: 3)
  1045. Conservation GST fusion proteins - (reply: 1)
  1046. Supernatant proteinprecipitation - (reply: 1)
  1047. Bradford Protein Assay - (reply: 2)
  1048. Protein size ! - (reply: 6)
  1049. SDS-PAge problem! - Protein separation (reply: 5)
  1050. Protein extraction from bacterial cells - Suitable protocol (reply: 1)
  1051. membrane protein purification - how to pufify cd3 protein from jurkat cell (reply: 1)
  1052. protein expression - What is a suitable strain (reply: 1)
  1053. proteins electrophoresis - (reply: 2)
  1054. how to precipitate the nucleicacid with out proteins - (reply: 2)
  1055. Hydrophobic proteins extraction? - (reply: 1)
  1056. Bradford Protein Assay - (reply: 1)
  1057. crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)