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Protein Related Discussions
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Purifying Phosphorylated protein - (reply: 2)
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Unstable protein - (reply: 1)
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Tool to investigate cleavage sites within a protein - (reply: 2)
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Seperating High MW proteins - (reply: 2)
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Problem with protein quantification - (reply: 2)
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Disappearing protein MWM marker - (reply: 1)
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Dephosphorylation of proteins in lysate - need a protocol (reply: 1)
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Purification of GST FUSION PROTEIN - (reply: 1)
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secretory proteins - recombinant proteins (reply: 1)
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Protein isolation by Trizol - (reply: 1)
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protein for braford assay and SDS-PAGE - (reply: 2)
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Can i send protein band from SDS-PAGE for MAlDI......... - What could be the complication!!!!!!!!!! (reply: 5)
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Expression of protein in non target strain but unfortunately no protein expressi - (reply: 1)
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protein in inclusion bodies-plz help - (reply: 4)
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Tricine - Fixation of protein (reply: 4)
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Protein Loading and Western Blot - Western Blot (reply: 5)
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optimum conditions for transfer of ~20-30 kDa protein - (reply: 1)
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single stranded binding protein? - how to put into my PCR reaction? (reply: 6)
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protein sequence data base - MS/MS (reply: 1)
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problem in protein expression after 8 months - (reply: 2)
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protein purification from media supernatant? - protein purification of ECM proteins (reply: 2)
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mysterious puzzle in result of confirmatin the protein expression after 3 years - (reply: 2)
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mitochondrial extraction immunoblot - what can be used for calibrating the amount of protein apart from VDAC (reply: 1)
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Protein Quantification: Laemmeli Buffer and nanodrop - Laemmeli Buffer and nanodrop (reply: 1)
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Really high enrichment... but equally high IgG? - How is this possible? (reply: 1)
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Exogenous protein expression - Problems in protein expression mammalian cells (reply: 2)
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EGFP cloning and membrane protein - (reply: 2)
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GST protein purification - (reply: 1)
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storage of purified proteins? - (reply: 1)
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Removing all cell surface proteins/receptors - (reply: 3)
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Protein Overexpression - Inquiring about growth time before induction (reply: 1)
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Pioneers of Mutagenesis to Probe Protein Structure and Function - (reply: 1)
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Immunoprecipitation, Protein A-HRP and Background - (reply: 1)
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heat retrieval - necessary or not? - his-tagged protein detection (reply: 3)
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Size of protein - (reply: 4)
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western blot: why are proteins not separated? - (reply: 4)
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expanded band of the purified protein on silverstain gel (even in low expression - (reply: 6)
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protein concentration - (reply: 4)
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FLAG-GFP-tagged protein problems - (reply: 3)
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troubles with small proteins (less than 20) on SDS page - (reply: 5)
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oligo modification - to prevent the binding of SSB protein (reply: 1)
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protein spreading in gel - (reply: 1)
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protein concentration measurement - (reply: 4)
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no expression of my target protein - (reply: 3)
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Does my protein bind nucleic acids? - (reply: 4)
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2D gelelectrophoresis: prestaining of proteins - (reply: 4)
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His-tag purified protein is yellow! - (reply: 7)
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general protein purification from mouse tissue - (reply: 4)
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protein structure alignment in 3D - (reply: 4)
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Different intensity present in reference protein even though BCA performed - (reply: 1)
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cell fractionation and low protein concentration in cytosol - (reply: 1)
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Technique to estimate the level of protein expression from media - (reply: 2)
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protein concentration of samples in Laemmli buffer - (reply: 2)
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unexpected 40kDa band -E.coli protein expression - (reply: 3)
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antibody against His-tagged protein - (reply: 3)
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Protein purification by two stage chromatography - MDH by anion exhange & gel filtration (reply: 1)
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protein/DNA methylation interaction - (reply: 5)
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Protein preicipitated after Dialysis - (reply: 7)
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Best storage temperature for samples - serum, protein, tissue, DNA, RNA... (reply: 5)
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Methods of concentrating Protein lysate - (reply: 2)
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transfer of high molecular weight proteins - (reply: 11)
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DNA motif database / no TF - database of DNA motifs for binding of DNA replication proteins (reply: 2)
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How to normalize ELISA measurements? - Total protein, cell number, or DNA? (reply: 1)
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1% boiling buffer for protein extractions - (reply: 3)
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Size of expressed Protein - (reply: 2)
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Thrombin cleaved GST-fusion protein degredation--suggestions - (reply: 2)
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Storage of samples for phosphorilated proteins - (reply: 1)
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Gene complexity and protein complexity analysis - (reply: 2)
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protein synthesis - wat happens in tranlation process? and afterwards? (reply: 1)
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protein extraction from mouse brain tissue - (reply: 1)
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GST-tagged protein expression problem-plz help - (reply: 3)
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pROTEIN DOMAINS - new to proteomics (reply: 3)
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Membrane protein problem - Problem in getting signals (reply: 3)
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Protein identification - (reply: 1)
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Troubles with expression proteins - (reply: 7)
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Phosphatase-Protease inhibitors in TCA total protein extraction - (reply: 2)
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HELP! protein extraction and centrifugation - (reply: 1)
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protein on membrane - (reply: 1)
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Protein Extraction and Centrifugation - (reply: 1)
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protein concentration problem - (reply: 2)
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E.Coli ceased to express my target protein - (reply: 5)
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protein purification from Pichia - Is there any good way to kill the protease activity? (reply: 2)
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problem with solubilizing protein in formic acid - (reply: 1)
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Finding an antibody for a weird species ! - What matters in protein blast? identity or positive? (reply: 1)
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Protein purification? - urgent (reply: 3)
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temperature can effect the protein active? - (reply: 1)
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protein gel electrophoresis - (reply: 7)
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storing dialysis tube(bag) - (reply: 2)
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treating dialysis bag with EDTA - (reply: 1)
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HIS TAG Protein Estimation - (reply: 5)
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Protein Activity Assay With 2D Gel Spots - (reply: 1)
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precipitation of protein with acetone - (reply: 3)
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question about the protein structure and function - (reply: 1)
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Protein Conc using Bradford Assay - (reply: 1)
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How to purify unknown protein? - (reply: 2)
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optimal timepoint to collect secreted protein - (reply: 1)
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Problem with GST tagged baculovirus protein - (reply: 2)
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Determining if a protein is a downstream target of another protein - (reply: 4)
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different splice variants - same protein (reply: 1)
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Fast Protein Liquid Chromatography (FPLC)/ HPLC - (reply: 4)
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Storage of supernatant that contain proteins after cell lysis. - (reply: 7)
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protein solubility - (reply: 2)
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Proteins recover from different buffer - How to obtain again proteins from ST buffer? (reply: 2)
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A problem with transient expression a myc-tagged protein in 293T cells - (reply: 1)
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native purification of His-tagged proteins from E. coli? - (reply: 1)
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Advantages of Proteomics Study - (reply: 1)
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GST tagged protein purification - (reply: 8)
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Cell surface Protein Isolation - Protein extraction procedure (reply: 4)
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problem dissolving protein pellet after aceton precipitation - (reply: 3)
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total protein extraction from brain tissue - (reply: 3)
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protein purification - (reply: 4)
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smeared proteins western blot - (reply: 5)
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a question in protein dialysis - protein dialysis (reply: 1)
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Protein come from nowhere - we cannot find RNA (reply: 5)
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8M urea buffer for protein extraction - (reply: 6)
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Do we have fluorescence genes? - genes which code for fluoscent proteins (reply: 12)
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what cell line to use for visualizing membrane localized protein - (reply: 1)
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what cell line to use for visualizing membrane localized protein - (reply: 1)
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how to pulldown membrane-bound protein? - co-immune precipitation (reply: 1)
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DNA "snot" in cell lysis/protein extraction - (reply: 6)
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A curious result- hide and seek with my protein - (reply: 13)
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Protein insolubility problem after bacterial induction - (reply: 2)
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how to improve protein expression - (reply: 2)
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TAT protein - (reply: 1)
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Protein detection and sensitivity of techniques - (reply: 4)
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Comparing surface protein expression of different cell lines - (reply: 1)
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Analysis protein expression - (reply: 1)
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Protein elution problems - (reply: 2)
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Bacterial expression antibody recognition problem - antibodies won't recognize protein (reply: 2)
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Comparative Genomics, Protein Sequencing, more - (reply: 1)
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islet protein extraction - (reply: 1)
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finding a protein's function - (reply: 1)
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Caspase 3 assay - on purified proteins (reply: 4)
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Negative Control Rabbit IgG for ChIP Assay - (reply: 3)
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Troubleshooting protein purification cation IEX - Cation IEX (reply: 1)
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DNA Protein pull down protocol for mass spec - (reply: 2)
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Protein size on SDS gel - (reply: 1)
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Protein ladder - (reply: 8)
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Protein purification - (reply: 1)
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amount of protein to load for a western? - (reply: 2)
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Protein expression using tap water? - (reply: 2)
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protein absorbance wavelength? - (reply: 1)
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IgG SDS-PAGE analysis - (reply: 1)
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Protein size prediction - (reply: 4)
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Protein purification issues - (reply: 4)
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Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
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p53 protein - design experiments to illustrate p53 protein character. (reply: 2)
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Coating Protein A and G on ELISA plate - (reply: 2)
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ultracentrifugation in membrane protein extraction - (reply: 2)
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Huge mRNA overexpression, but no protein overexpression. - (reply: 10)
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Best Method for Target Protein Quantification - Western Blot Accuracy Improvement Needed (reply: 7)
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about transfer low molecular weight protein in tricine PAGE - (reply: 5)
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exogenous and ectopic protein expression - what's the difference (reply: 2)
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protein isocratic HPLC.. preparatin of tris buffer + n-propanol mix - getting variations in Retention time.. (reply: 1)
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Separation of cytosol and membrane proteins by (ultra)centrifugation - (reply: 5)
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19kda-15kda-6kda.. resolution of these proteins? - which gradient sds-page would be best suitable? thanks! (reply: 2)
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finding accurate protein molecular weight - protocol? (reply: 3)
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Protein expression problems in HB101 - (reply: 1)
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Is there a way to extract RNA and protein from the same sample? - (reply: 2)
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protein expression problem - (reply: 1)
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How to select 1 Ab for detect interest protein - (reply: 2)
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protein extraction protocol? - (reply: 3)
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Akta prime protein purification - I need advice (reply: 2)
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Identifying a protein isoform - (reply: 1)
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where can I know the isoelectric pH of the following proteins? - (reply: 3)
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Trying to detect correct membrane protein size in gel - At wits end (reply: 4)
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Semi-Dry transfer large protein - (reply: 3)
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transfer problem - i cannot transfer proteins to a pvdf membrane (reply: 7)
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protein elution - (reply: 6)
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isotope labeling of proteins - (reply: 4)
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protein precipitation - (reply: 2)
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TRIZOL for protein extraction - any problem? (reply: 4)
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protein pKa prediction site - (reply: 3)
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Elution problem in Protein G column - Guide me (reply: 2)
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precipitation of protein (PI=9.5) but not DNA - I need protein free sample for DNA quantification (reply: 2)
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proteins expression - (reply: 1)
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MASCOT results vs 2D gel image : Which one do you trust? - Protein positive hits in MASCOT do not agree with a spot's position!! (reply: 2)
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Experience with protein G dynabeads? - (reply: 2)
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About preparing solution for protein biochemistry - (reply: 2)
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Protein Informative Tools - (reply: 1)
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Recombinat Insoluble Protein Expression in E.coli - Inclusion body foramtion (reply: 1)
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URGENT: software tools for proteomics - (reply: 1)
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protein concentration - calculation question (reply: 2)
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Why Proteins? - Can't figure proteins out, let's find some help? (reply: 4)
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separating protein and nucleic acids - problem separating protien and nucleic acids (reply: 8)
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DMSO for protein stability at -80oC - protein storage (reply: 4)
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protein A IP - (reply: 1)
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How to separate very close protein bands - (reply: 5)
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how to find mol wt. of specific protein on net? - (reply: 2)
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size exclusion.. how to reduce tailing. for proteins.. - sillica matrix tsk columns.. (reply: 2)
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Size exclusion chromatography for resolution of 18 kd protein and 15 and 10 kd d - columns for the range? (reply: 3)
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oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
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troubleshoot the protein expression - different protein size but the same construct (reply: 1)
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getting part of a protein sequence - finding protein domain sequences (reply: 1)
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pIs and proteins - (reply: 5)
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purification of non his-tagged proteins - (reply: 4)
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Problems with zymography - Running SDS page gels protein wont migrate down the gel (reply: 4)
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Sample buffer and protein assay - (reply: 1)
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native and oxidized protein.. separation on Ion exchange chromatography? - possible? method suggestions? (reply: 2)
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protein extraction fromn soil bacterium - (reply: 1)
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protein extraction from mammalian cell line - preferred protocol? - (reply: 2)
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how to find the modification enzyme of a target protein - (reply: 1)
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ELISA - how to make a protein lysate? - (reply: 3)
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Residues don't match... why? - in human and mouse proteins (reply: 2)
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Antifungal Proteins - (reply: 1)
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How many ways are there to conc. protein? - (reply: 5)
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protein expression in E.coli - no expression after induction (reply: 1)
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6XHistaq proteins - (reply: 2)
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protein precipitation after enterokinase cleavage - (reply: 1)
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minimum protein conc for immunoprecipitation - what's the minimum needed for results (reply: 1)
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Precipitate proteins but not 1kDa peptide? - (reply: 3)
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Seperation of unconjugated protein after hapten conjugation - (reply: 1)
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Using Smith-Waterman for comparison of protein sets - (reply: 2)
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Protein purification - (reply: 5)
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Protein Quantitation - Methods thats are used for measure the low protein concentation (reply: 9)
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Protein Extraction from SDS-PAGE - (reply: 1)
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protein colour problem - (reply: 2)
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probing one protein with multiple antibodies - (reply: 2)
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Different between Protein A and G beads - (reply: 3)
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Acid hydrolysis of protein - (reply: 1)
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dialysis - (reply: 2)
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Subcellular localization of -HA tagged protein - (reply: 1)
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cohesive-end ligation - cloning a fusion protein to a vector (reply: 1)
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DNA binding proteins ELISA - need suggestions (reply: 1)
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His Antibody - Is any worked with 4xHIs tag protein (reply: 1)
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Western for a 500kda protein - some troubles, need help! - (reply: 1)
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Identify crosslinked protein with mass spectrometry ? - (reply: 1)
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Inclusion bodies protein determination - (reply: 1)
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what are the methods available for protein extraction from cell line? - (reply: 6)
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How to make my protein Soluble? - Bacterial system it sucksss ? (reply: 1)
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Protein A and protein G - (reply: 4)
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Protein homology modelling - (reply: 3)
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GST or His tag for protein purification? - Tag choise + native vs denaturing conditions (reply: 2)
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wrong protein size - (reply: 6)
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Influence of pressure fluctuation in ion exchange column on protein binding, sep - (reply: 2)
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Mysterious protein degradation - (reply: 4)
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protein extraction from yeast--all extracted proteins are below 50KD - (reply: 2)
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protein extraction from yeast--all extracted proteins are below 50KD - (reply: 1)
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protein extraction from yeast--all extracted proteins are below 50KD - (reply: 5)
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Acid in Dialysis Tubing - probably a stupid question (reply: 2)
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How can I avoid protein precipitation? - (reply: 7)
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Which control protein for Western Blotting of nuclear protein? - (reply: 9)
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Checking recombinant protein size - (reply: 3)
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Protein sequence alignment - (reply: 4)
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express toxic protein in insect cells - (reply: 2)
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Protein isolation from RNAlater stored tissue - (reply: 5)
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Measuring intracellular protein concentration - (reply: 2)
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How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
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How to deal with insoluble proteins? - (reply: 2)
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Point Mutation, Protein Folding, Base Pairing - (reply: 3)
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Protein Isolation of very low cell number... - (reply: 4)
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Protein extraction from periplasmic space - a simple protocol anyone? (reply: 1)
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HELP:protein over-expression failure?! - (reply: 1)
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membrane protein extraction - membrane protein extraction (reply: 7)
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No mRNA or protein made by my mutant gene - (reply: 4)
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membrane protein and boiling - (reply: 3)
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Best Fluorescent Protein - (reply: 2)
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protein band is shifted after boiling sample with sample buffer - (reply: 7)
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protein band is shifted after boiling sample with sample buffer - (reply: 4)
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Dialysis, how long? - (reply: 8)
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Reverse phase chromatography of acetone protein precipitate - (reply: 2)
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Extracellular protein expression in E.coli - anyone knows a good commercial vector? (reply: 1)
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Can anyone recommend a good IgG Ab for negative control for ChIP? - (reply: 2)
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Removing histone proteins from nuclear protein isolation - (reply: 2)
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Trouble expressing 155 kDa protein in pET21a - (reply: 3)
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Bradford Assay - Calculation protein concentration (reply: 9)
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Which quantity of purified protein in WB? - (reply: 2)
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Which quantity of purified protein in WB? - (reply: 1)
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protein extraction - (reply: 4)
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Protein questions - (reply: 6)
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Why my target protein did not show up, but the non-specific binding showed up - (reply: 2)
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Elisa- need help - Elisa - Phosphorylation studies (reply: 1)
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Concentrating protein - (reply: 5)
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Dialysis Buffer for Recombinant Protein - (reply: 5)
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2D Electrophoresis for protein inclusion bodies/lysate? - sample preparation? (reply: 1)
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protein recovery problems in analytical size exclusion chromatogrphay - or how do i have a more sensitive method? (reply: 3)
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Intracellular proteins on live cells - (reply: 3)
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to explain why i see two forms the same protein in SDS-PAGE - (reply: 1)
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drug targeting: G-proteins or protein kinases? - (reply: 1)
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Smeary western blots of cell lysates - western of a HMW membrane protein (reply: 2)
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optimize TRIzol protocol for RNA/DNA/Protein, HELP? - small amout of input material (reply: 1)
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Frozen Protein A agarose beads - (reply: 1)
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Using 4-parameter fit for colorimetric assays for protein concentration? - how appropriate is it.. higher concentraion.. no linearity? (reply: 1)
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protein inclusion bodies.. purpose of multiple water washes after lysate centrif - (reply: 5)
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cannot find the expression protein - (reply: 5)
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protein degredation - (reply: 3)
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How can I increase protein expression in S. cerevisiae? - (reply: 6)
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protein purification from yeast - (reply: 2)
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poor electrophoretic protein transfer - (reply: 5)
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Problem in concentrating protein - (reply: 1)
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Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
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Reverse phase chromatography - protein sample preparation and loading - (reply: 1)
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How to increase expression of protein in yeast? - Western from yeast and liquid beta-galactosidase assay (reply: 1)
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protein absorption at 260nm - (reply: 7)
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Sample (protein) recovery on 2D gel - (reply: 1)
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can't view the protein band..help! - (reply: 1)
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Fusion moieties for fusion proteins - (reply: 1)
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unstable protein expression,pleeeeeese help - (reply: 6)
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Why a previously soluble protein is now going into inclusion bodies? - protein expression (reply: 4)
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cleaving protein - (reply: 8)
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protein extraction with phenol/SDS - (reply: 5)
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protein estimation colorimetry.. preferred range.. - statistically more precise & accurate? (reply: 2)
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His-tagged protein not pure - (reply: 8)
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Recombinant protein stability - stability issue (reply: 1)
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good transfection efficacy, no proteins in WB - the weirdest problem of my life (reply: 1)
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Western blot is not working with stable cell line=please help - no expression of my protein detected (reply: 5)
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protein expresses using pGEX4T-3 but not being purified? - trapped in GST (reply: 8)
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How to find miRNAs for one designated protein? - miRNA (reply: 1)
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IF - Methanol Vs. Triton? - Cytoskeleton and Membrane associated proteins (reply: 2)
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transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
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RPC column pore size versus protein size - (reply: 1)
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protein normalizer (equal protein loading) - (reply: 3)
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Membrane protein - (reply: 11)
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ddephosphorylating proteins using acid phosphatase - (reply: 1)
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Regarding Protein Stability - (reply: 1)
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Fishin out possible interacting protein for known RNA..?! - (reply: 1)
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interested protein shows in every sucrose fraction! help! - (reply: 3)
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Protein extraction supernatant turns black/sooty color - (reply: 4)
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checking protein purity in SDS-PAGE - evaluating the protein purity analysing the 1D SDS-PAGE (reply: 2)
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Protocol for IgG Purification from Ascites? - (reply: 1)
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ProteoJuice Protein Transfection - (reply: 2)
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experimental determination of protein function - (reply: 2)
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Getting RNA and Protein out of/off from Matrigel - (reply: 4)
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How to separate proteins of equal size - (reply: 3)
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Use of protease inhibitors when the target protein could be a protease that inte - (reply: 2)
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Using urea in ion exchange chromatography of proteins - (reply: 1)
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How to demonstrate protein-protein interaction - How to demonstrate that a protein is a receptor (reply: 10)
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upscaling protein production - is type of vector and host strain crucial factors? (reply: 1)
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Free trial kit for DNA, RNA, and protein extraction - AquaRNA - an aqueous DNA, RNA, protein extraction solution (reply: 4)
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protein tag for ICC/IF - (reply: 2)
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how to solubilize out functional membrane protein - (reply: 4)
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cytosolic protein collection - (reply: 3)
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physical properties of protein - (reply: 2)
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Is there a way to screw up dialysis? - (reply: 8)
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Protein Purififcation - (reply: 3)
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protein extraction from Daphnia/rotifera - (reply: 3)
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protein refolding from IB.. why done at alkaline pH? - (reply: 1)
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immobilized protein affecting activity - (reply: 4)
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Protein solubility test - (reply: 1)
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Oligomeric protein - how to measure concentration? - (reply: 2)
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How to make a neat gel for master thesis? - Expressed protein on SDS-PAGe gel (reply: 3)
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his-tag protein pure but degradated during growth - his-tag protein pure but degradated during growth (reply: 15)
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Recombinant protein solution turns yellow when concentrated - (reply: 3)
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precipitation of guanidine HCl-sample after adding 4X laemmli buffer - SDS-PAGE protein sample preparation (reply: 2)
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Protein precipitation. Overdrying proteins - (reply: 3)
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Protein expression changes after freezing+thawing - (reply: 1)
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SYPRO RUBY protein blot staining and Subsequent Western blot - (reply: 3)
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to cross-link a protein to a polysaccharide, which to choose? Sulfo-NHS or NHS? - (reply: 5)
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purification failed - how to restart? - Protein diluted in many fractions (reply: 7)
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programmes for protein sequences characterization - (reply: 1)
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how to determine protein size in sucrose graident? - (reply: 10)
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band size with flag tagged proteins? - (reply: 3)
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Microsome protein concentration - (reply: 4)
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identification of secretory protein - (reply: 1)
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Isolation of secreted proteins - (reply: 2)
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Immunization with protein band from SDS-PAGE - (reply: 5)
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No protein expression in pKK223-3 vector in JM105 - (reply: 3)
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protein extraction - cytosolic lysis buffer (reply: 8)
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Elisa for total human IgG from culture supernatant - (reply: 1)
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protein elution - any idea to elute protein (reply: 5)
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protein elution from SDS PAGE - any idea how to elute protein from SDS PAGE (reply: 1)
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caspase-3 cleavage assay - how to test the clevage of a protein by caspase in vitro (reply: 2)
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Which proteins are in Triton X-100 soluble and insoluble fraction? - (reply: 4)
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what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
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protein folding - (reply: 2)
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protein concentration - (reply: 1)
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protein denaturation - (reply: 3)
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bands sizes with sectreted protein - (reply: 1)
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GST tag protein purification - (reply: 2)
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smearing of proteins during transfer - (reply: 10)
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Antigen Anti body and Protein - (reply: 3)
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contamination of DNA in protein sample - (reply: 1)
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interaction of viral protein with RNAi machinery - (reply: 1)
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analysing protein sequences - How to find domains? (reply: 5)
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protein extraction - (reply: 1)
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Gentle extraction of nuclear protein complex - (reply: 2)
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Transfer of large molecular weight proteins - Complete transfer of large molecular weight proteins (reply: 10)
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Protein into the membrane - (reply: 2)
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western transfer efficiency.. with or without methanol / SDS? - any differences? for what kindof proteins? (reply: 1)
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do i need to desalt my protein after purification - (reply: 1)
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protein sequence - (reply: 1)
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DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
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What is the pI of most of proteins in cell? - (reply: 3)
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reverse phase hplc of proteins.. mechanism of binding and elution.. - your views.. ! (reply: 3)
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selection of buffer for dialysis? - (reply: 1)
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BSA vs. Nonfat dry milk for serine/threonine phosphorylation sites - which is better for quality blots? (reply: 1)
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how to improve the gst protein purification? - (reply: 1)
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Western Blot for weakly expressed protein - (reply: 3)
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RIPA for DNA Isolation - Can you do simultanous isolation of protein and DNA with RIPA (reply: 3)
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My protein shows 3 bands - Reb1 phosphorylation? (reply: 1)
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separation limit of proteins? - (reply: 1)
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A ROS producer that doesn't cause protein carbonylation - (reply: 2)
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Western-Blot: wrong MW for my proteins (synaptohpysin and SNAP-25) - (reply: 3)
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Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
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Purifying protein with no his tag - (reply: 5)
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large quantity of high concentration protein needed - (reply: 3)
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Stained Protein Markers - aberrant molecular weights (reply: 3)
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resolving 27 kDa protein - (reply: 3)
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Confirmation of protein expression in pCDNA3 - How to know foreign gene expression in pCDNA3 (reply: 8)
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small volume spectrophotometrical protein quantification - (reply: 1)
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why need to do protein assay before western blot? - (reply: 3)
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HELP: protocol for protein extraction - (reply: 4)
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Crossreactivity or protein complex under denaturing conditions? - (reply: 2)
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Killing bacteria without denaturing proteins - (reply: 9)
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Solubilizing protein from E. coli inclusion bodies - need help!!! (reply: 3)
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immunohistochemistry and protein denaturation - (reply: 3)
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Protein expression in S.cerevisiae - (reply: 4)
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How Conserved are the "Constant" regions of 'IgG' Heavy and Li - (reply: 5)
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How to normalize protein vs its phosphorylated form? - (reply: 2)
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Protein is not binding to the ion-exchange column. - (reply: 4)
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protein size determination? - (reply: 33)
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programme to predict amphipathic helices in a protein aminoacid sequence - (reply: 1)
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Can IP shift the MW of a protein? - (reply: 2)
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How to tackle the problem of protein denaturation while storing? - (reply: 10)
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Suppression seen at protein levels but not at RNA - Pls Help - (reply: 5)
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Just a smear of proteins after ponceau red - Western Blot (reply: 4)
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Storing protein extracted from tissues for Western blot - (reply: 9)
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Storing tissue for protein and RNA extraction? - (reply: 13)
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How to quantify protein which has sds buffer already - (reply: 2)
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different western results when using acetone prep vs crude protein - (reply: 3)
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protein expressed once,but stopped expressing then - (reply: 1)
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Gap43 protein western Blot - (reply: 7)
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Imaging GFP tagged proteins, background signal due to contamination? - Imaging, background spots/speckles, contamination (reply: 6)
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post-translational modifications which increase molecular weight of the protein - (reply: 7)
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Problem with protein extraction - (reply: 2)
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SDS Gel - My protein bands go to the bottom of gel (reply: 9)
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2 - DE Gel - Protein Transfer - (reply: 2)
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how to refold insoluble protein after purification - (reply: 10)
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oligos phosphorylation with PNK - (reply: 6)
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10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
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Problem with expression of DsRed fusion protein. - (reply: 4)
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how to express GST fusion trunctated protein? - GST fusion protein (reply: 3)
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Extraction of protein from inclusion bodies - (reply: 1)
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G protein inhibition - (reply: 3)
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Dialysis ? - (reply: 1)
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Isolation of golgi transmembrane protein - (reply: 4)
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Question: What purpose does bromophenol blue serve in my protein isolation? - protein isolation (reply: 3)
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protein nature in different pH? - (reply: 3)
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protein G, binding buffer pH? - (reply: 3)
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protein determination in loading buffer - (reply: 1)
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Ethanol precipitation of protein - (reply: 2)
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oligos phosphorylation with pnk - (reply: 4)
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Discrepance between peaks in chromatogram and amount of proteins? - (reply: 1)
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problem in expression of target protein - (reply: 11)
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how to remove detergent from protein sample - (reply: 4)
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concentrating low expression protein for western - will this work? (reply: 3)
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Protein synthesis in primative organisms - Archaebateria or extremophiles (reply: 1)
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Protein Refolding problem - (reply: 2)
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DC Protein Assay - (reply: 3)
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Consistency problems with Micro BCA assay - (reply: 4)
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protein storage.. pH? - ideal pH.. near/far pI of the protein? (reply: 1)
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Buffer Exchange & Protein Purification - (reply: 1)
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His-tag protein purification- buffers to use - What buffers to choose for His-tag chromatography (reply: 2)
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Do I need to do BCA assay to quantify the protein before doing the ELISA? - BCA assay and ELISA (reply: 9)
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Strange run or transfer of proteins at 15% PAGE (not band but spot) - (reply: 2)
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storage buffer for purified protein - (reply: 3)
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Use cold TCA for protein precipitation? - (reply: 3)
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new techniques about protein ladder? - (reply: 3)
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protein an its phospho-protein in the same membrane - (reply: 3)
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Solubilisation of proteins out of membranes - Which detergent at what concentration (reply: 1)
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How can I load equal amounts of protein into each well during SDS-PAGE? - SDS-PAGE, WB, BCA assay, Actin (reply: 7)
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marker proteins for plasm membrane in c2c12 myoblasts - (reply: 1)
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Concentrating dilute protein samples for use in elisa - (reply: 2)
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protein array - (reply: 1)
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production of monoclonal antibody when the host protein is having sequence simil - (reply: 3)
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EMSA for non-DNA binding protein - (reply: 1)
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how to quantify total protein in Elisa? - Elisa (reply: 1)
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who has done biotin pull down RNA interacting protein before? - biotin pull down RNA interacting protein (reply: 3)
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Deletion mutant protein prediction - (reply: 3)
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Protein expression in Gram(-) bacteria - Protein expression by using special S-D sequence (reply: 1)
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Quantification of Diluted Protein - (reply: 4)
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MAJOR PROBLEM IN EXPRESSING GST TAGGED PROTEIN - (reply: 1)
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protein salt precipitation - (reply: 2)
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Problems with inconsitant protein recovery using ammonium sulphate (AS) precipit - protein precipitation/concentration (reply: 2)
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Why I could only see knockdown in mRNA level but protein? - help (reply: 3)
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Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
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help! proteins families - (reply: 3)
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What is the best protein expression system - (reply: 1)
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SDS-PAGE of secreted protein - (reply: 9)
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Big problem in GST Protein purification - (reply: 9)
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agents to break hydrophobic interactions.. - how to distinguish disulphide vs hydrophobic protein protein interacti (reply: 1)
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Protein extraction from animal cells - (reply: 7)
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protein purification - (reply: 5)
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preparing samples for phosphorylation sites identification - (reply: 3)
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protein travels differently under different SDS-Page conditions - (reply: 4)
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Trying to get protein out of insoluble fraction - (reply: 3)
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Membrane protein extraction from mice tissue - (reply: 3)
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getting rid of protein above 60 kDa - (reply: 7)
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protein expression in NSO cells - (reply: 6)
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Suggested tool for protein analysis? - (reply: 4)
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solublization of basic protein - (reply: 3)
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What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
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protein expressed in two elutes, which to use? - (reply: 2)
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Protein G purified IgG>>> extra band at approx 200 kd - (reply: 5)
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Concentrating recombinant protein without dialysis - (reply: 5)
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How to stain protein fiber to view in light microscopy? - (reply: 1)
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Is TCA really specific in precipitating proteins from cell lysate? - (reply: 1)
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Protein reversed phase analysis.. linear or step gradients? - which is preferred and why?? (reply: 3)
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Does phosphorylation affect protein migration in SDS-PAGE gel? - (reply: 9)
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protein isolation from cell lines - membrane bound proteins (reply: 3)
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protein extraction - (reply: 1)
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Protein Extraction - (reply: 1)
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Calculating protein size - how to calculate the protein size when cloned in pet vector (reply: 1)
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Can you get two proteins made from a heterozygous gene mutation? - (reply: 3)
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disappear protein band after development - (reply: 11)
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Any good method for resuspension protein from TCA/acetone precip ? - (reply: 4)
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Membrane Protein Extraction and Analysis Questions - (reply: 1)
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SDS-page & lipidated proteins - (reply: 6)
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Proteomics - Determination of structure of Protein. (reply: 2)
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Long PCR primers for epitope tagging - where to order? (reply: 3)
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Western blotting for membrane bounded protein - (reply: 7)
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Separation of piperidine from proteins after AIC - (reply: 1)
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Indirect IgM ELISA Background - (reply: 1)
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IgG purification: Multimerization... - (reply: 4)
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DNA, RNA, protein acetone precipitation? - (reply: 2)
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Protein crashing out of solution - (reply: 4)
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Is Co-IP with natural protein a fantasy? - (reply: 6)
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Protein expression in E.coli DH5alpha? - (reply: 1)
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Protein cocentrators / centrifugal filter tubes - (reply: 3)
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question about protein molecular weight - (reply: 12)
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brightest FACS fusion protein - (reply: 1)
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Ab's production from recombinant proteins (2) - new idea (reply: 2)
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how to identify significance of a protein interaction - (reply: 1)
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how to identify the significance of a protein - (reply: 1)
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buffer for anion exchange chromatography of basic proteins - (reply: 1)
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Ab's production from recombinant proteins - (reply: 4)
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Protein expression help needed - Mammalian cells (reply: 11)
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how to define an appropriate protein reaction buffer? - (reply: 1)
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Western Blot conditions for high molecular weight proteins - (reply: 2)
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Probelm with expression of ~85 kDa GSt fusion protein. - (reply: 5)
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Protein Loss - Losing Protein During Desalting (reply: 13)
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amount of protein for MALDI-TOF - (reply: 5)
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stickiness of protein - (reply: 1)
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Electro Elution of my protein after SDS-PAGE - (reply: 7)
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How can I resuspend membrane proteins for further inmunizations? - (reply: 1)
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Western for membrane bound protein - (reply: 11)
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Epitope tagging - for the lazy bones - (reply: 3)
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Size Exclusion Column - Proteins not coming out ! - BMP-2 (30 kDa) is inhjected in a colum (size exclusion at 100kDa) and (reply: 2)
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SDS-PAGE: Small protein diffuse and not staining well - (reply: 5)
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Help for sample preparation for 2-D - How can I get the protein for IEF (reply: 1)
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who can help me with staining with 80kD to 130KD protein family? - it is very difficult for me to successfully staining with integrin (reply: 14)
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antibody against yeast protein - (reply: 1)
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Lyophilisation of protein lysates - (reply: 4)
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In immunoprecipitation, how to elute proteins without IgG disturb? - (reply: 2)
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His tagged protein purification - (reply: 2)
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My fusion protein's reading frame disrupted? - Please help...... (reply: 4)
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Immonuprecipitation of membrane proteins, is it possible? - (reply: 1)
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Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
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Problems with HMW proteins and Western blot - (reply: 5)
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ChIP - a couple of Q's about real time validation - Too much background? Compare to IgG or Input? (reply: 1)
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how to deal with protein degradation - (reply: 12)
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Epitope tagging - Several basic questions (reply: 2)
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Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
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Prtocol for large scale protein expression - (reply: 1)
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Protein precipitation compatible with Sucrose gradients? - What precipitation protocol wil function with a high % of sucrose? (reply: 5)
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Methods needed for gene/protein identification and function - (reply: 2)
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Protein quantification - (reply: 3)
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detecting small membrane protein by western blot - (reply: 4)
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Protein quantification with BSA? - (reply: 2)
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losing protein expression after stablizing cells( please help) - (reply: 4)
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native or denatured protein? - for antibodies (reply: 5)
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Please help me! I am really tired of this stupid protein purification! - (reply: 8)
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more protein can give a band shift on a SDS-PAGE? - (reply: 1)
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wetern for membrane associated protein - (reply: 1)
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How to keep protein in Phosphatased Form - Run Co-IP need to have protein in phosphatased form (reply: 4)
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Protein Sample - How to store it? (reply: 1)
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protein polysorbate 80 separation method? - (reply: 3)
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protein marker for 2D-PAGE - (reply: 2)
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Antibodies for different epitope giving bands of different sizes in the same sam - (reply: 1)
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[In vitro Kinase assay] Do phosphorylated proteins migrate more in SDS-PAGE? - (reply: 3)
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High MW smear - how can I get all protein into the gel? - (reply: 3)
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Measurement of protein- protein interaction? - (reply: 2)
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Bradford vs 280 protein quantification - BSA standard vs cell lysates (reply: 4)
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HPV E7 protein - (reply: 1)
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IgG light chain amino acid sequence from pigs/rabbits/humans? - (reply: 1)
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Rabbit/pig/human IgG Light chain amino acid sequence needed - (reply: 1)
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Protein Expression problem - N-End Rule?? (reply: 1)
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IP first-timer - doubts on equal protein concentration control (reply: 2)
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Protein will stripped by overwahsing? - (reply: 12)
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no protein expression - (reply: 2)
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Protein Expression Problem - N-end rule?? (reply: 2)
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Protein stability in acetonitrile - storage conditions? - (reply: 1)
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ELISA standard curve - approximate protein concs to use? - (reply: 3)
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Sensitive protein assay - (reply: 2)
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Mycobacterial protein extraction... without chemicals! - (reply: 3)
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timing - DNA to RNA to Protein (reply: 1)
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Superdex or Sephacryl? - protein purification by size exclusion chromatography (reply: 7)
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when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
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Does trypsin digest integral plasma membrane proteins? - (reply: 2)
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protein band is shifted after incubation with plant protein extracts! - (reply: 2)
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protein complex purification taptag - (reply: 2)
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passage of cell culture and protein locolization - (reply: 1)
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ELISPOT: - peptide or protein (reply: 1)
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positive IgG in ChIP - (reply: 2)
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protein precipitation - basic question (reply: 1)
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What is the most effective method of protein precipitation? - (reply: 2)
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The best way to confirm an protein-protein interaction? - Between a protein and an intergrator (reply: 4)
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protein precipitating - (reply: 8)
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GST fusion protein expression problem - (reply: 1)
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Dialysis doubt - (reply: 1)
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[western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (reply: 2)
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protein estimation of monoclonal antibody - inconsistent values (reply: 2)
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Mab purification - no mouse IgG band seen (reply: 3)
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WB trouble - no signal of low kd protein - (reply: 5)
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Low weight protein - (reply: 5)
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what's the difference between the purification of GST-fused protein and His- - (reply: 4)
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The secret? cytosolic proteins adherent macrophages? - (reply: 1)
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validating the efficiency of inhibitors of protein kinases A & G - (reply: 3)
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Special procedures for phosphorylated proteins? - During isolation and western blot (reply: 3)
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HELP estimation of protein in ammonium sulphate purified igG - (reply: 6)
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GST pull-down:Is there any way to reduce nonspecific binding of protein to gluta - (reply: 3)
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Protein G beads for IP - (reply: 1)
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protein expression level of stable transfectant will change? - (reply: 1)
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please help: how to isolate nuclear protein from tissue samples - (reply: 1)
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mechanism of protein denaturation caused by organic solvent - (reply: 2)
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lysis buffer for coip with a membrane protein - (reply: 2)
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collect secreted proteins in cell culture media - (reply: 2)
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Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
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Removing proteins by dialysis - want to get rid of proteins in a vesicle suspension (reply: 2)
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Antibody not binding to protein A/G - Immunoprecipitation (reply: 3)
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IgG separation - (reply: 1)
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Is it OK to use monoclonal antibody against its host protein for westen - (reply: 4)
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Different endogenous protein detection result in western and IHC - (reply: 3)
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problem in centrifuge of protein - (reply: 3)
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Nuclear Lysates Protein Quantification - Nuclear Extracts (reply: 4)
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for protein refolding - (reply: 5)
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how to find immunogenic peptides in a protein? Help! - (reply: 1)
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protein kinase C (PKC) inhibitors - (reply: 1)
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Remove albumin from purified protein sample? Help please - (reply: 5)
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Size exclusion chromatography for 23 and 20.5KDa? - protein purification (reply: 3)
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Protein extraction using trizol - (reply: 2)
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extract proteins for WB - (reply: 4)
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Amplify XCX15 random peptide library for epitope mapping - (reply: 1)
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TCA radiacive protein precipitation without lysis - (reply: 1)
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PCR of mitochondrial fraction of nuclear-coded mitochondrial proteins - (reply: 4)
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How to chose vector and E.coli strain for protein expression - (reply: 2)
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Looking for an antibody/epitope type of search... - (reply: 1)
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Methods for large-scale protein purification - (reply: 5)
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Protein Immobilization - Protocol for protein immobilization on a silanized glass substrate? (reply: 2)
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Could protein modification block an antibody epitope? - (reply: 4)
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Renin protein detection in Western blotting - (reply: 2)
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How do i organize immunoblotting for 24 proteins from the same sample? - (reply: 4)
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salt precipitation (for protein/collagen) - (reply: 1)
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how to investigare new protein - (reply: 2)
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Low amount of protein - (reply: 1)
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protein extract questions - (reply: 1)
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Eukaryotic recombinant protein expression - (reply: 3)
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Yeast protein extract preparation for a quick western blot - (reply: 5)
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Control protein in culture medium for Western? - (reply: 3)
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Loss of LMW in Protein Standard - (reply: 2)
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Protein expression vs. protein activity? - (reply: 2)
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promoter regulation but not protein regulation - (reply: 4)
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Can i recover protein from a stained SDS-PAGE? - (reply: 7)
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why express mamallian proteins in DH5alpha ? - (reply: 1)
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How to study a protein in proteasome sys - (reply: 5)
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Protein expression - (reply: 1)
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His fused protein - (reply: 2)
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Stability of protein back bone? - (reply: 6)
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Protein precipitation during dialysis - (reply: 7)
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Problems experienced from adding 3xHA epitope to the C- terminus of some interes - Problems experienced from adding 3xHA epitope to the C- terminus of so (reply: 2)
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Elution of His-tag Protein under denaturing condition - (reply: 2)
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ELISA-amount of total protein to coat wells - (reply: 2)
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secreted proteins purification - how to concentrate a secreted protein contained in conditioned medium? (reply: 4)
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Protein extraction-Lysis Buffer - WB (reply: 1)
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my protein gives huge tailing.. - rp-hplc for a protein (reply: 1)
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SOS for GST-fusion protein expression - (reply: 1)
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How to lyophilize protein - (reply: 8)
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Find a known domain a protein? - (reply: 3)
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NaOAc versus MgOAc? - protein extraction (reply: 1)
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reuse of Protein G in IP procedure - (reply: 2)
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Different size protein then expected - (reply: 2)
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plasmid prep with protein? contamination - (reply: 3)
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Question about purification of GST-taqqed protein - (reply: 2)
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Temperature effect of protein? - (reply: 5)
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How to backtrack to DNA sequence from protein - (reply: 4)
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Protein loading control? - (reply: 4)
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How to express toxic protein in BL21(DE3) or (pLysE) strain - (reply: 1)
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how can I get enough proteins for Mass spec? drive me mad! - (reply: 12)
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Genes/Proteins - (reply: 6)
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Identify one protein among several - (reply: 4)
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western blot for phosphorylated proteins - (reply: 3)
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IgG purification - amonium precipitation method (reply: 5)
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dialysis questions - (reply: 6)
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problem with overexpression - mRNA vs protein level (reply: 2)
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Linker design - Design of linker to fusion two protein domains (reply: 1)
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3D protein structure - (reply: 2)
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SDS-PAGE problems: proteins not running entire legnth - (reply: 7)
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What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
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Dialysis - (reply: 9)
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phospho protein - (reply: 11)
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protein purification problem - (reply: 3)
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IgG molecule - molecular weight (reply: 2)
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Protein expression from bacteria which has two plasmids? - Incompatibility of plasmids (reply: 5)
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antibody purification - how to get rid of bovine IgG? (reply: 3)
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Calculating protein concentration - (reply: 5)
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tips for detect phosphorylated proteins - (reply: 2)
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Protein not entering inclusion bodies - (reply: 3)
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transfected protein sequestered inside vesicles - (reply: 3)
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westerns, protein concentr and RIPA buffer - (reply: 2)
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Western Blots with (250 kDa) High Molecular Weight Protein - (reply: 2)
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total protein assay before western blot - (reply: 1)
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Trouble finding 5kDa protein in western blot - (reply: 5)
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how to remove PEG in my protein sample - (reply: 1)
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Larger sized Protein Standard Ladder Req - (reply: 1)
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Protein purification with HPLC - (reply: 6)
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identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
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Protein in each elution step during His-Tag purification - (reply: 4)
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GST-fusion protein purification from bacteria - Several questions on protein purification (reply: 3)
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how to efficiently elute protein from Protein A-agarose beads? - (reply: 5)
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anti IgM secondary HRP? - (reply: 1)
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DsRED fluorescent protein - (reply: 7)
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cell lysis to investigate phosphorylated protein - (reply: 1)
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isolation a protein complex - (reply: 1)
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protein ppt removes nucleotides? - (reply: 3)
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Actin + another protein - (reply: 3)
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How do you know if a specif Ser of the substrate protein is phosphorylated by a - (reply: 3)
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Protein precipitation with PEG 4000 - (reply: 4)
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isolation of protein complex by use percoll gradient - (reply: 1)
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How to quantify wild-type and mutant protein? - (reply: 4)
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siRNA causing no protein knockdown - (reply: 1)
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Overexpressed protein runs slower than endogenous - Difference in MW (reply: 2)
-
mass spectrometry analysis protein identification drive me crazy - (reply: 9)
-
Membrane proteins extraction protocol - (reply: 2)
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How to renature denatured protein? - (reply: 4)
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protein m wt high - can amino group of lysine side chain form bond with free corboxyl ter (reply: 6)
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Separation of protein size of 20kDa and 150kDa - (reply: 4)
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Calculation of MW fusion protein - (reply: 1)
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Can membrane proteins be separated using 2D-PAGE?!?! - (reply: 2)
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Puzzled__How to identify the interaction of two protein! - I had disclosed FGF inhibited the X protein.But I can not explain the (reply: 6)
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Help--how to isolate the monomer proteins - (reply: 3)
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WB: protein amount/ lane ? - What is the optimal protein amount per lane in WB ? (reply: 6)
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Protein purification - (reply: 2)
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protein stability issues - (reply: 4)
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40kDa protein for ladder - (reply: 4)
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How to extract protein from a bone marrow sample - protein from bone marrow (reply: 1)
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loading control for nuclear protein in rabbit cell extract - (reply: 1)
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protein concentration for ELISA - (reply: 1)
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Vector for expressing proteins to be secreted - (reply: 3)
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Vector for producing Fc fusion proteins - (reply: 1)
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Protein Extraction from cell media for western blot - (reply: 3)
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Immunoprecipitation of a secreted protein - (reply: 5)
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N-terminal protein sequencing.. "holes" at lysine residues.. meaning? - what kinda modifications cud've happnd? (reply: 2)
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using IgM antibodies in IP - (reply: 3)
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Western Blot for Higher molecular weight Proteins - (reply: 1)
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how to reduce unspecific binding of proteins to the beads.. - IP-purification (reply: 3)
-
HELP! contaminants do not separate from my protein during purification - (reply: 5)
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Total cellular protein from lysate? - (reply: 7)
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Protein Expression Problem - (reply: 5)
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Removal of detergent from protein solution - (reply: 3)
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How to measure protein expression levels of multiple forms of glycosylated prote - (reply: 2)
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Protein Fluorescent Tagging - (reply: 6)
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Protein from Liver tissue - (reply: 2)
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Simple Protein Extraction compatible with Bradford - (reply: 1)
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Problem with Protein purification - Protein seems to stick to sepharose (reply: 6)
-
Protein quantification on spinal cord lysates - reproducibility issues (reply: 1)
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Estimating Protein Mass - Best way to quantitiate protein (reply: 1)
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What is difference among Protein A, protein G, and Protein A/G? - (reply: 1)
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Phospho protein % in the cell - signal transduction (reply: 1)
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Protein concentration problem ! - (reply: 14)
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Total proteins extraction - (reply: 2)
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protein dilution - (reply: 3)
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Epitope mapping - (reply: 5)
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Need to increase protein precipitation yield w/ TCA precipitation protocol - (reply: 3)
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Surface proteins isolated by biotinylation are contaminated.. - (reply: 3)
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WB for secreted protein? - (reply: 5)
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membrane protein western blot - (reply: 3)
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Protein Crosslinking - Tryin' to Crosslink 2 proteins using -SH group (reply: 4)
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where can I access the all known proteins express in brain? - perhapes a database , but where to find it? (reply: 4)
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2D gels: Is there a protein assay thiourea is compatible with? - proteomics (reply: 2)
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Protein purification - (reply: 2)
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mutation in a gene that is not expressing a protein - (reply: 4)
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Incomplete transfer of proteins for western blot - (reply: 4)
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biotinylation of IgG - (reply: 2)
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how to avoid cytosolic proteins in nucleus - (reply: 3)
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Protein G - (reply: 1)
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Mammalian protein expresion in bacteria - Which plasmid and strain to use (reply: 7)
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How can I purify the wanted protein - (reply: 10)
-
pET IPTG expression - Getting protein expression (reply: 1)
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Recombinant protein - (reply: 4)
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PRotein marker can give concentration of my protein in gel? - (reply: 4)
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Protein expression with IPTG - (reply: 6)
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Gel shift with high isoelectric point protein - (reply: 1)
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Proteinase K + surface localization of proteins? - (reply: 1)
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ChIP IgG has the same signal than the antibody tested - (reply: 9)
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Problem with SDS-PAGE-diffuse protein bands - (reply: 4)
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Protein lysis buffer for mass spec - recipe (reply: 15)
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Adrenergic receptor proteins (western blot problems) - (reply: 10)
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peptide phosphorylation analysis method - (reply: 3)
-
Sudden loss of protein expression in E coli - (reply: 3)
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does low mRNA expression mean low relevant protein expression - looking for answers or references (reply: 9)
-
Western Blotting for Large Proteins - (reply: 2)
-
Hard to dissolve pelleted protein after TCA precipitation - Urgent!! Please check this out!! (reply: 2)
-
GST fused protein purification - (reply: 4)
-
Cloning short sequences - Does PCR can help clones upto 70 bps into a protein sequence (reply: 10)
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How do I choose a 96-well plate for BCA assays? - (reply: 4)
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Question for purchasing purified protein - (reply: 3)
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protein extraction form SDS PAGE gels - (reply: 4)
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Enough protein for mass spectrometry - How much is enough? (reply: 2)
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protein and proteomics - western blot of small peptides (reply: 3)
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Protein cleavage site? - (reply: 1)
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protein extraction from wheat seed - total protein extraction (reply: 2)
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MW marker for 150 kDa protein in Western blot - (reply: 5)
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Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
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Correcting phospho-protein with total protein - (reply: 2)
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same protein=opposite effects - (reply: 1)
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Protein insoluble in 8M urea or 6MGuHCl - (reply: 8)
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promlems in protein purification in native form - (reply: 2)
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purification of proteins from sample - (reply: 12)
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antibody against denatured protein? - basic questions (reply: 2)
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how can I dialyze protein through its PI and avoid precipitation? - (reply: 1)
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Why the protein can not be tested? - (reply: 2)
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questions on protein glycosylation - (reply: 2)
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protein purification - (reply: 5)
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Immunoprecipitation & Mass Spec analysis - How much mg(ug) of protein lysate is needed for IP followed by Mass Sp (reply: 2)
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Is 20% methanol sufficient to fix protein in gel? - (reply: 5)
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dialysis tubes for chromatin purification - (reply: 5)
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mutant protein expression & purification problem in E.coli - Protein with five residues mutated to Alanine's (reply: 6)
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Protein expression. Question of V5 epitope and 6x His tag ! - (reply: 2)
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soluble protein, low expression - (reply: 6)
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EGFP fusion protein expression - Expression of a GFP fusion protein in E.coli (reply: 4)
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Total protein extraction from Leaf tissue - (reply: 1)
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protein chromatography - (reply: 5)
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His tag protein binding to resin ... - can i purify off the resin? (reply: 5)
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Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
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Leaf protein extraction - (reply: 5)
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CHIP assay shows same fold enrichments over IgG in both my gene promoter and bet - CHIP negative control (reply: 2)
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About the protein enginerring - In urgent, thanks (reply: 1)
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CFP-protein - fuses proteins (reply: 1)
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protein concentration determintaion - (reply: 2)
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2D-PAGE PROTEIN ANALYSIS - SECRETED PROTEIN DESALTING (reply: 4)
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Carrier protein in target protein - (reply: 5)
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Gel Loading- Equal Protein or equal volumes? - (reply: 2)
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How to elute protein from SDS-PAGE gels - (reply: 3)
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How to test the relationship between secreted protein and signaling pathway - (reply: 4)
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G418 effect on protein concentration - (reply: 1)
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loading control for mitochondrial protein - (reply: 1)
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Rotating proteins? - (reply: 3)
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Protein expression Using NEB yest kit - (reply: 1)
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How to determine protein tagging level? - (reply: 2)
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GST fusion protein preparation protocol and troubleshooting - (reply: 1)
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prob with biorad protein standard - (reply: 1)
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Protein Solubility - How to determine? (reply: 2)
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why did the membrane protein disapear again? - (reply: 3)
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E coli protein extraction for EMSA - (reply: 1)
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Isolation membrane proteins in "cell-friendly" buffer - extracted proteins need to be tested in in vitro models (reply: 1)
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Plant Protein Extraction - (reply: 2)
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false positive clones URGENT - resitant to G418 but not expressing protein (reply: 13)
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Chemiluminescent protein marker - (reply: 3)
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Cannot get any protein overexpression in Rosetta? - (reply: 1)
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Silver stain Protein detection - Silver stain problem (reply: 4)
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anyone had elute protein from PVDF and use it for mass spec? Thanks - (reply: 3)
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Preventing protein aggregation - (reply: 2)
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western blot of phosphorylated proteins - (reply: 4)
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Method to determine whether protein exists as Monomer/Dimer etc.? - (reply: 6)
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cell wall protein extraction of gram-positive - (reply: 1)
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half life of EGFP protein - (reply: 3)
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Silver staining of crystals - To prove it's a complex between 2 proteins (reply: 1)
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subcellular fractionation confirmed by western not consistent with microscopy - nuclear protein by microscopy not nuclear in fractionated sample -WHY? (reply: 3)
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Renaturing Protein after Acid Elution - (reply: 4)
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Transfer optimization of membrane proteins to PVDF - (reply: 3)
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IPTG protein expression in BL21 (or orthers) - how to measure the expression (reply: 1)
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protein precipitated - (reply: 2)
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Ask for recommendation of GST fusion protein purification kit - (reply: 1)
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Phosphorylated Proteins - (reply: 3)
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TX-100 resistant protein - (reply: 1)
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How to purify protein secreted in medium - (reply: 2)
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Nature of total proteins can influence the migration? - (reply: 6)
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Protein isolation - Small size His-tag Protein isolation (reply: 4)
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same proteins, same antibodies, different results - (reply: 1)
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Protein purification - (reply: 3)
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Seleno protein P - Help needed in estimation protocol (reply: 1)
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protein extraction from SDS-PAG (GeBAflex) - your opinion about usefulness (reply: 1)
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Integration of a nucleotide sequence within an alignment of protein sequences - Managing the frameshifts... (reply: 3)
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Why the DNA sequence big different but protein the similarity? - such as MAOA and MAOB genes (reply: 4)
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how to see low kd protein in vitro transcription/translation? - (reply: 4)
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Loss of phosphorylation after gel purification? - (reply: 3)
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How to the modification of a protein? - (reply: 2)
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How to determine protein concentration - how to determibne particular protein concentration from a tissue homog (reply: 2)
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Protein G problem - Antibodies absent from both flow through and fractions (reply: 4)
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Retinoic Acid Receptors Protein Extraction - (reply: 2)
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Good review about protein expression in E.coli - (reply: 1)
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Protein purification - (reply: 3)
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overexpression of a protein from a HA tagged plasmid - western blot detection (reply: 2)
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protein extraction from muscle biopsies - protocols needed (reply: 3)
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Bacterial strains used in protein expression - (reply: 5)
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Protein high molecular weight standards for gel filtration - (reply: 2)
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Is a protein of 24KD too small to serve as the antigen? - help! (reply: 3)
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How many methods can demenstrate to endogenous protein interactio? - (reply: 3)
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gst tagged protein expresion - (reply: 3)
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Anybody has a good phosphorylated IKKa antibody? - (reply: 3)
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Larger protein size in SDS gel - why is it like tat? (reply: 11)
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will freeze thaw cycles or sonication disrupt protein interaction? - (reply: 3)
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Purification of GST-fusion - GST-fusion protein: How to express and purify (reply: 7)
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Protein storage and BSA - (reply: 1)
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Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
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A CoIP problem - How to reduce the non-specific binding of Protein A beads? (reply: 4)
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Resolve High Molecular Weight Proteins in SDS-PAGE - (reply: 3)
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SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
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a question about screening transfected cells by GFP protein - (reply: 5)
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native protein - (reply: 2)
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After successful Transfection protein not detectable - (reply: 3)
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concentration of protein in urea - I need help to concentrate proteins (by precipitation?) that are in 8M (reply: 5)
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Visualising 200-400+ kDa proteins by gel electrophoresis - (reply: 3)
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Serum ELISA IgG titer quantitation - How to determine IgG titer without known sample? (reply: 4)
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In vitro translation>how much protein? - What is the average amount of protein from IVTranslation? (reply: 9)
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Does protein A affect Protein B level in my case? - need advice on my confusing result (reply: 7)
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Protein A column still okay if it was let dried? - (reply: 6)
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Specific and Total Activity for Protein Purification - (reply: 2)
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Need help:preparation of protein complex - (reply: 1)
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Purified protein has no activity - (reply: 8)
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HAEMOGLOBIN: the intelligent protein - (reply: 8)
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Protein purification after cleavage (thioredoxin) - (reply: 3)
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suggestions to express protein in e coli - (reply: 3)
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Isolating c. elegans protein free from bacterial's - (reply: 1)
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protein complex purification, western shows tag protein was pulled out, but silv - (reply: 10)
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CIP and protein - (reply: 2)
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Western Transfer and Detection of small protein - (reply: 6)
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2D protein gel for miRNA target screening? - (reply: 2)
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protein expression from glycerol stock - (reply: 2)
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confusing shape of survivin protein band - (reply: 3)
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Peg..How to rid myself of it - Peg bound to my protein (reply: 2)
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protein concentration - bradford method (reply: 9)
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Expression of an N-terminally tagged golgi protein - (reply: 1)
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size of tagged proteins - (reply: 3)
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How to detect protein kinase from lysate - (reply: 4)
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Which water is better for chemical preparation ? - water grade for protein purification (reply: 2)
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SDS Page running problem - prestained protein marker - (reply: 5)
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Fusion protein without stop codon? - (reply: 1)
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large proteins don't analyse well-sds page gels - (reply: 12)
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large protein degradation - during baculovirus purification (reply: 2)
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High concentration of imidazole, protein stability - (reply: 4)
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truncated protein under normal conditions. - it is possible? (reply: 6)
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Western Blot total loaded protein amounts per lane - (reply: 4)
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how to recover my protein? - A terrible mistake (reply: 9)
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Troubleshooting protein expression - GST tag - (reply: 3)
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Quantifying Proteins, help required! - (reply: 4)
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What type of native gel (pH/acrylamide %) do I use for a protein with the pI=9.0 - (reply: 4)
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His-tag protein expression problem - (reply: 4)
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mass spectrum how much protein is enough for it? - (reply: 5)
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Protein A agarose for CHIP--pre-treatment is needed? - (reply: 2)
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2DE of hydrophobic membrane protein (problem) - (reply: 3)
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How do I prepare a sample to quantify a certain protein with ELISA? - (reply: 10)
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problem of specific Thr phosphorylation detection - (reply: 1)
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specific ser/Thr phosphorylation detection by in house antibody - (reply: 1)
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MMP9 protein zymography - (reply: 5)
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Immunostaining of cytoplasmic proteins in nucleus, why? - (reply: 10)
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how to isolate non-crosslinked protein? - (reply: 6)
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protein degradation - (reply: 2)
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Ethanol precipitation of protein sample - What is the protocol? (reply: 4)
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Recombinant IL-2 proteins.. - Work for cancer.. how? (reply: 1)
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Types of aggregates in a protein.. Disulphide linked? Hydrophobicity related? - Conditions that can make or break them? (reply: 5)
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transfer gene to protein? - (reply: 3)
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How to purify a sticky protein - (reply: 1)
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COS-7 and PC-3 transfected with a GFP-AR fusion protein - (reply: 4)
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Reasons for wrong protein size on Western blot and how to deal with - (reply: 8)
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REFOLDING - visualisation of protein after denaturation step? - (reply: 1)
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Accuracy of protein quantitation methods - (reply: 1)
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protein extraction from blood sample - (reply: 2)
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GFP Fusion protein - (reply: 6)
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Problem with transfection and protein expression - NO DIFFERENCE BETWEEN UNTRANSFECTED AND TRANSFECTED CELLS (reply: 10)
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Easiest protocol for protein extraction from C.elegans - (reply: 2)
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Expression in codon plus of GST fusion protein - (reply: 3)
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Removing serum proteins bound to Plasma membrane? - (reply: 9)
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Unreliable Protein Induction - (reply: 1)
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Specific Protein Database - Website? (reply: 2)
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2 questions in 1....about transferring large proteins...and blotting - (reply: 7)
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Overexpressing bacterial protein in mammalian cells - which vector to use? - overexpression in mammalian cells (reply: 9)
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Question about phosphorylation and ligation - (reply: 1)
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typical cell lysate protein concentration - (reply: 6)
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Purification after oligos phosphorylation? - (reply: 1)
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Pull down assay---- to study which proteins interact with the "bait" - Which technique is good? (reply: 3)
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Questions about protein gel loading and immunoprecipitation - (reply: 4)
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I have done a SDS-PAGE to find the mass of the unknown protein... - (reply: 4)
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Formaldehyde Cross-Linking for protein - (reply: 1)
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why quick freeze-thaw cycles can improve protein recovery? - (reply: 3)
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How to get rid of nuclease in protein preparation? - (reply: 5)
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YFP fussion protein problem - (reply: 2)
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Why BSA can stabilize proteins? - (reply: 8)
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I need a recombinant protein in high amounts - what company would you talk to? (reply: 6)
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EMSA with recombinant protein - (reply: 2)
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Structure based multiple protein alignments - How can I improve my alignments if I have a partial structure? (reply: 4)
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CBB DYE BOUND TO PROTEIN - (reply: 2)
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visualizing purified protein - (reply: 9)
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his-tag protein expression - inoculation of large cultures with plate colonies/ starter cultures vs. glycerol (reply: 1)
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why protein gels are run at constant current and constant voltage? - (reply: 2)
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Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
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phosphorylation of a protein - (reply: 6)
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protein precipitation upon dialysis - (reply: 5)
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I miss two Proteins from my overexpression in BL21 DE3 - (reply: 1)
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ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
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Lots of bands on immunoblot for an expressed protein - (reply: 8)
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faster proteins - (reply: 1)
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protein separation - (reply: 7)
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Big protein - (reply: 6)
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Purification protein from inclusion bodies - (reply: 4)
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protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
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Elcetrophoresis Question ( Protein Size) - (reply: 5)
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in vitro translation - how is the protein purified? (reply: 12)
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red fluorescence protein - (reply: 1)
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mobility of protein band over PAGE - please suggest (reply: 1)
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How to remove nuclease contamination from protein prep? - (reply: 1)
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getting rid of contaminant proteins in a Ni column using imidazole - (reply: 2)
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Purification of protein from plasma - How to separate a 30kDa protein from serum albumin (reply: 2)
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PHOSPHORYLATION - (reply: 15)
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Low transfection efficiency/protein expression - I've tried everything! (reply: 7)
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precipitation of nuclear proteins during GST pull down assay - (reply: 1)
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Are miRNAs phosphorylated at the 3' end? - Labelling of miRNAs in a T4 RNA ligase reaction (reply: 3)
-
Protein concentration measure with NanoDrop - (reply: 22)
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Transfection and Expression: getting transcript but no protein expression - (reply: 3)
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Protein extraction from soya meal using membrane - (reply: 1)
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Failure in Immunoprecipitation - No target protein found in eluate (reply: 4)
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Strange: mRNA expression goes up but protein goes down - (reply: 2)
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changing dialysis buffer - (reply: 7)
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Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
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Immunoprecipitation of reduced proteins using Pierce kit - Immunoprecipitation using Pierce Kit (reply: 4)
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Purified Protein Aggregation/Multimerization Problem - (reply: 3)
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Fluorescent proteins mPlum - (reply: 1)
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Protein analysis - (reply: 4)
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Any kit out there for protein and RNA isolation from monocytes? - (reply: 4)
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Protein purification before mass-spec - need sample purification methods (reply: 4)
-
Anti-digoxigenin Immunoprecipitation of Proteins - (reply: 3)
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Rabbit IgG Isotype Control for Co-ip - Need available antibodies (reply: 1)
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Stable vs transient - which gives the highest yield? - I'm setting up a expression system for a secreted protein, should I choose s (reply: 2)
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what these components do in the buffer used in protein purification - (reply: 5)
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protein folding- looking for renaturation buffer - (reply: 1)
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protein folding- looking for renaturation buffer - (reply: 1)
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ELISA Ab question - denatured protein and extract - If I extract denatured protein... (reply: 2)
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Ultrasonic homogenizer for protein and enzyme extraction? - (reply: 3)
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Will the membrane-associated protein aggregate after the removal of detergent? - (reply: 1)
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Low protein yield with immunoprecipitation - (reply: 3)
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Fusion protein questions - Botched deletion primer (reply: 2)
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heating of samples during plant protein extraction - (reply: 2)
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Will Yeast2Hybrid work on membrane proteins - (reply: 2)
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Protein extraction from SDS gel - (reply: 6)
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Protein loading control for RAW cell SUPERNATANTS in western blot - western blot (reply: 7)
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Protocol for protein isolation from culture media - (reply: 3)
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Protein loading control for RAW cell supernatants - Western blot loading control (reply: 2)
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detecting transmembrane protein exoression for monoclonal ab production - (reply: 1)
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guanidinehydrochloride vs. guanidineisothiocyanate - Is there a difference concerning ability to denature proteins? (reply: 2)
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acetone for protein precipitation - grade of purity (reply: 3)
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Protein A or protein G for co-IP? - (reply: 3)
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SDS-PAGE protein aggregation and precipitation - (reply: 1)
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how to remove detergent from protein sample? - (reply: 5)
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Protein extraction/lysis from whole tissue - Do you have a protocol? (reply: 3)
-
who knows (nucleolar organizer region-associated proteins) - (reply: 1)
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Is ChIP is a good choice to find the DNA sequence for a binding protein? - (reply: 2)
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problem in protein purification phenol by phenol extraction - (reply: 1)
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too much protein for Western? - (reply: 5)
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WB for comparing the level of certain protein in two different cell lines - (reply: 2)
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Low molecular weight protein problem - (reply: 11)
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CHO culture: what media and additives? - for stable Fc fusion protein expression (reply: 4)
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Binding partners to protein of interest - (reply: 1)
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IP a protein from human serum - (reply: 4)
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specific protein in high quantities - what can I do? (reply: 3)
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Does Ammonium Sulfate Fraction of Protein and untrafiltration effect to enzyme a - (reply: 2)
-
How much can I believe protein MW standards? - (reply: 10)
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Help with protein extract! - (reply: 7)
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Does SDS loading buffer + heat completely denature protein? - (reply: 3)
-
protein expression in BL21 cells - problems (reply: 7)
-
Fusion protein expressing problem - (reply: 2)
-
investigation of protein stability using cyclohexemide - (reply: 3)
-
What is the maximum amount of protein that could be loaded in WB? - (reply: 2)
-
which company can raise good antibodies? - raising antibodies using the native protein (reply: 3)
-
G protein coupled receptor - western blot - (reply: 1)
-
G protein coupled receptor - western blot - (reply: 3)
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I want to strip the protein off PVDF membrane - (reply: 4)
-
protein predictive tools - (reply: 2)
-
GFP labelled protein purification - (reply: 1)
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LOW concentration protein assay - help! (reply: 5)
-
Periplasmic protein extraction problem! - (reply: 2)
-
Protein refolding - (reply: 2)
-
Protein sample preparation for SDS-PAGE - (reply: 9)
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What size needles I should use for cell extraction protein - (reply: 1)
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Epitope tag positioning - (reply: 3)
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Help with protein extraction! - WI-38 cells (reply: 1)
-
Protein expression - decreased expression (reply: 2)
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Options for protein expression? - (reply: 3)
-
western - cells treated w/ protein synethesis inhibitor - (reply: 1)
-
protein structure prediction - (reply: 2)
-
microgram to micromolar conversion - Protein concentration - microgram to micromolar conversion - Protein concentration (reply: 4)
-
How to lyse plant nuclei? and keep nuclear protein complex stable - (reply: 1)
-
How to normalize the protein expression amongst different tissues - (reply: 7)
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Problem with protein transfer in non-reducing conditions - (reply: 4)
-
cancer-specific proteins - (reply: 1)
-
Protein samples not transferring over to membrane - Western Blot (reply: 13)
-
Maltose Binding Fusion Protein MBP - which strain for expression? (reply: 1)
-
translation of mRNA into protein - (reply: 1)
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can we purify the protein from westernblot membrane? - (reply: 5)
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What molecular weight cutoff dialysis membrane to use for virus concentration - (reply: 11)
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Total protein in human serum - (reply: 2)
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acidic proteins in SDS-PAGE - (reply: 3)
-
PROTEIN AND DNA GEL ELECTROPHORESIS - GEL ELECTROPHORESIS (reply: 4)
-
the voltage or current of transfer membrane for 200~300 kd protein - (reply: 3)
-
amount of proteins isolated from cells - (reply: 2)
-
Accelrys Discovery Studio - a software for protein modelling - (reply: 3)
-
Western Detection of Transcription Factors - -more difficult than other proteins? (reply: 3)
-
why couldn't I see my protein on western blot after ChIP? - (reply: 3)
-
protein preparation for Western Blot - (reply: 6)
-
Trizol protein extraction - (reply: 2)
-
Medium for adherent cells and serum/protein free - (reply: 2)
-
Bacteria protein extraction - (reply: 1)
-
protein concentration measurement - (reply: 12)
-
protein samples running in a straight line - (reply: 11)
-
Secreted protein degraded in conditioned medium? - (reply: 1)
-
Protein gel Loading troubles - Protein prep too viscous (reply: 4)
-
How can I express my protein successfully? - (reply: 10)
-
Phenol, Proteins and DNA - (reply: 1)
-
how can i use SDS and Triton X-100 to isolate the membrane protein? - (reply: 6)
-
HOw can i isolate the membrane protein? - (reply: 9)
-
differential electrophoretic migration of phosphorylated proteins - (reply: 2)
-
Western Blot on an IEF separated recombinant protein - (reply: 5)
-
can protein A agarose precipitate anti goat IgG? - (reply: 2)
-
how to denature protein from multimeric form into monomers - (reply: 4)
-
Maltose Binding Protein - Information on maltose binding protein... (reply: 5)
-
FLAG-Tag Protein expression in MCF-7 - (reply: 8)
-
protein ladder detection for western blotting - (reply: 2)
-
Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
-
Detecting phosphorylated PKR - antibody and positive control - (reply: 4)
-
PMSF and tissue culture - Can you apply PMSF purified proteins to cultured cells? (reply: 2)
-
Selection of best recombinant protein produing strain - selection of industrial strain (reply: 3)
-
is there any fast screening after siRNA knockdown of protein? - (reply: 3)
-
May a CARRIER-antibody compete between them for binding to protein G-agarose? - Immunoprecipitation problem (reply: 10)
-
~25kDa UPP (Unidentified Purified Protein) after HIS-purification - (reply: 1)
-
cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
-
protein expression in pET-30a vectors - (reply: 2)
-
NF-kB EMSA - Different nuclear protein extraction method leads to opposite results (reply: 1)
-
protein production didn't work! - (reply: 19)
-
thrombin cleavage and triton? - protein purification (reply: 2)
-
Verification of protein level change by RT-PCR - the results are just not compatible (reply: 8)
-
protein extraction - protein extraction from wheat seed (reply: 4)
-
Cloning of truncated protein. - Do I need to include the stop codon? (reply: 11)
-
How do you purify membrane protein? - help (reply: 2)
-
Software for proteins! - (reply: 3)
-
Is it possible to do siRNA followed by transfection of dn of the protein? - HAs anybody tryed this?? (reply: 3)
-
Transfection of 293 cells for protein expression - optimum conditions for transfection!!! (reply: 5)
-
Protein level in fibroblasts cell line - (reply: 1)
-
His tagged protein won't elute from nickel resin - (reply: 4)
-
two plasmids of the same protein with tags on N and C-terminus. - how can I make use of them? (reply: 3)
-
Which way will my protein run? - Show off knowledge of pI (reply: 1)
-
protein precipitation - (reply: 4)
-
Protein purification with sephadex? - (reply: 2)
-
Protein immobilization on nitrocellulose membrane - (reply: 4)
-
How long can a protein last on membrane? - (reply: 10)
-
protein insoluble in just about everything! - (reply: 3)
-
Protein aggregation (HELP!) - (reply: 3)
-
Protein in active form - (reply: 2)
-
how to check the degradation rate of a specific protein - (reply: 2)
-
SDS-PAGE - CAN I USED 12% GEL FOR WHEAT LEAVE PROTEIN SDS-PAGE (reply: 1)
-
5' phosphorylated primers - (reply: 1)
-
Small fragment RNA isolation from interacting protein - (reply: 1)
-
Multiple bands on Western blot for an expressed protein - (reply: 7)
-
MBP fusion protein... - (reply: 6)
-
far western - Would the expression of the protein in mammalian cells be enough for far wester (reply: 2)
-
Protein formed ppt after stored at -80oC - (reply: 3)
-
Protein lysis buffer - Choice of buffer for different proteins (reply: 1)
-
to check protein expression - (reply: 4)
-
Overexpression of Viral Protein in mammalian cell line - (reply: 3)
-
Time point for reduction of protein levels after RNAi - (reply: 1)
-
are there any good system to express functional mammalian protein? - I tried using E.coli, but protein is not functional (reply: 15)
-
quanitation of protein extracted with trizol - (reply: 7)
-
Protein Sorting - The models of Protein sorting (reply: 1)
-
unspecific binding of protein a sepharose - immunoprecipitation problem (reply: 1)
-
NF-kB protein nuclear and cytosol isolation - No band separation in cytosol and nucleus (reply: 7)
-
trouble getting cysteine labeled with biotin-maleimide - cysteine scanning purified protein (reply: 1)
-
How to get rid of DNA contamination in protein samples - (reply: 3)
-
how to determine whether protein level increase is resulted from mRNA stability - (reply: 1)
-
Proteins samples not migrating from stacking into resolving gel - (reply: 6)
-
Protein TCA Precipitation Resolubilization Question - (reply: 3)
-
Plotting graph - On Microsoft Excel for protein quantitation (reply: 3)
-
Biotinilated proteins. Problems with Hens buffer - (reply: 2)
-
Verify the protein spots found by 2D using Western blot - The expression level change are different (reply: 2)
-
about protein domains in pfam - (reply: 4)
-
Protein Phosphorylation - (reply: 1)
-
problem of protein expression - problem of protein expression (reply: 1)
-
Protein extraction-what's in the pellet? - (reply: 6)
-
Protein Transfection by Electroporation - (reply: 6)
-
Lost: protein. Last seen prior to purification. - not present in flow through, washes or elutions! (reply: 2)
-
sds-page of hemocyte lysate proteins - (reply: 2)
-
Precepitation during dialysis - (reply: 6)
-
Protein expression troubleshooting - problem in expression of my protein (reply: 3)
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GST-tagged protein purification. - inclusion body formation of my GST taggged protein. (reply: 3)
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Question about the strategy of protein purification - (reply: 4)
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How to detect the protein concentration by UV light - (reply: 2)
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protein dialysis - (reply: 1)
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Thrombin - pDEST20/GST-Baculovirus - Cloning and Protein expression (reply: 3)
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Why should add DTT and what kind protein assay I should use - (reply: 4)
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Problem with protein refolding - protein precipitate all the time !!! why (reply: 2)
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One or two bands with different protein extraction buffer - (reply: 3)
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Coomassie Blue protein assay - Interference from phenolic acids (reply: 4)
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Membrane protein extraction - What about MEM-PER kit (Pierce)? (reply: 1)
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Pierce BCA analysis problems :/ - (reply: 3)
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Protein Structure - (reply: 4)
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Failed to express a protein - (reply: 5)
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Protein forced aggregation - conditions? (reply: 1)
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Protein refolding from Inclusion bodies - how to do and confirm the folding? (reply: 4)
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A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
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Protein characterization - what kinda stuff do u do? (reply: 1)
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Protein crystals - (reply: 3)
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Mouse IgG purification - Whether to use Protein A or Protein G column (reply: 2)
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Overexpression of 630 aa protein - (reply: 3)
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Membrane protein extraction - Qiagen extraction kit? (reply: 2)
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IP from frozen protein extracts - (reply: 10)
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membrane protein aggregation - (reply: 2)
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How to confirm a protein is a receptor? - (reply: 6)
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protein concentrates on surface of Nickel resin! - (reply: 2)
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Concentrate protein from dilute solution - (reply: 2)
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Selenium is the only nutrient element that has direct relation with viruses and - - A study on the hypothesis that deficiency of selenium proteins will lead to th (reply: 3)
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Protein Precipitation (wessel and Fluege method) - 2 questions (reply: 7)
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How to get rid of contaminated nucleases from purified proteins - (reply: 2)
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Cleaving a protein from GST - Troubleshooting methods to liberate my protein from gluthathonine sepharose (reply: 3)
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vector for express secreted protein in vivo - (reply: 3)
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protein purification -- 2 or more columns - (reply: 9)
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Protein precipitates - (reply: 7)
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protein folding - GST-fusion protein fluorescence (reply: 2)
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protein interaction study - (reply: 3)
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what buffer will prevent the precipitation of protein at -20? - (reply: 5)
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Use of Urea instead of SDS to denature protein for Western? - (reply: 2)
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what is the best method to concentrate protein - (reply: 2)
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Protein Purification - (reply: 1)
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protein for crystalization - (reply: 1)
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I accidentally put two start codons on my protein . . . - (reply: 3)
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How to convert the OD of protein to its concentration? - (reply: 2)
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I cannot get protein from my stable transfection cell - (reply: 4)
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Protein structure comparison- sortware needed - (reply: 2)
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Detergent for soulble protein - (reply: 5)
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problems with Immunoprecipitation- Western Blotting - Rabbit IgG secondary givi - (reply: 1)
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western blot loading control for secreted saliva proteins - (reply: 2)
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Develop a way to retrieve frequent protein segments by suitable algorithm - program, idea and so on . (reply: 1)
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my protein is 18Kda but runs as a 25kda on WB - why ? - (reply: 13)
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how to remove media components from protein solutions - (reply: 4)
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If is Kozak sequence(ccacc) needed when expressing a foreign protein in pEGFP-N1 - (reply: 2)
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Expression of fusion protein in mammalian cells - (reply: 2)
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How to remove BSA from protein sample? - (reply: 7)
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Membrane protein extraction and detection - General lab tech (reply: 1)
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GST + 6kD protein expression? Doesn't seem to be working! - (reply: 9)
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How to elute more protein from flag M2 beads(sigma)? - in hurry (reply: 6)
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How can I extract Chromatin-protein complex without using Chip? - The cheapest method to prove a protein to bind DNA (reply: 3)
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Cleavage of target protein from glutathione sepharose - (reply: 3)
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Protein Purification - Problem in protein purification (reply: 6)
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After cells are treated with TCA, how to resolve the protein in cell pellet? - (reply: 4)
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small protein - (reply: 2)
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protein protein interaction - (reply: 3)
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help-SDS-PAGE of heavy proteins - (reply: 7)
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Perl script - parsing non-phosphorylated protein sequences from a Uniprot flat f - (reply: 12)
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Concentrating proteins for N-terminal sequencing - (reply: 2)
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How does protein precipitate? - (reply: 1)
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His-tag protein is lost - (reply: 5)
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biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
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Blue Native Gel Electrophoresis for membran protein complex - (reply: 1)
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Proportion of folding proteins among all 100 AA long proteins - (reply: 1)
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determining if a recombinant protein is being secreted - (reply: 3)
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detection of a secreted recombinant protein - (reply: 2)
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good program to model 3D changes to protein from mutation - (reply: 1)
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IEF for pI 9.4 protein - ? (reply: 1)
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Western Blot Analysis - Technique for Protein determination (reply: 6)
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general protein confimation in lysis buffer - effect of reducing agents (reply: 5)
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Epitope tags - (reply: 1)
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protein precipitation during concentration - (reply: 5)
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SDS-PAGE - did not find the protein band what I expect (reply: 3)
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PEG to seperate Plasmide from Protein? - (reply: 2)
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phosphorylated protein Western blotting - HELP!! (reply: 10)
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making large proteins - (reply: 6)
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how to label all proteins after western-blot - someting like ponceau red, but after blocking (reply: 6)
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buffer for His-tag recombinant protein purification - (reply: 2)
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Any experieces with unstable primary antibodies? - still recognizes recombinant protein but not native... HELP (reply: 1)
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Losing Low Molecular Weight Proteins in SDS-PAGE - (reply: 2)
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easy protein extraction from Pichia pastoris - (reply: 3)
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Protein from blood samples - (reply: 5)
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GST fusion protein purification - (reply: 9)
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Aggregated proteins in SDS-PAGE - (reply: 5)
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Two to use PCR to get domain deletion within a protein? - (reply: 2)
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recommendation on mitochondria matrix protein antibody? - (reply: 1)
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Protein acetylation - (reply: 3)
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SDS protein sample over boiled, what happend? - (reply: 1)
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Proteins and markers in top of gel - Running a gel (reply: 5)
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how to purify fusion proteins on a large scale? - (reply: 2)
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The biology activity of espressed protein - (reply: 3)
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separation of two negatively charged proteins.... - ion exchage chromatography??? (reply: 2)
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protein leftover in DNA - how to get rid of it? (reply: 4)
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how can I increase protein expression level - hurry (reply: 2)
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protein consensus - (reply: 6)
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concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
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lysis buffer to extract transmembrane protein - (reply: 3)
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BCA protein assay - (reply: 3)
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Protein determination - Old Skool style - I'm looking for a decent descriptive protocol for protein detection using th (reply: 6)
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Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
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Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
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how to do western with 500 kD protein? - (reply: 5)
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DsRed fusion protein not glowing - It is there by WB though (reply: 3)
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stable or transient transfection method? - for protein expression (reply: 4)
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Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
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Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
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extreme conditions to induce protein? - im ready to do anything (reply: 2)
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Transfer high molecular weight protein - (reply: 4)
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What epitope is my antibody recognizing? - (reply: 3)
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his-tag protein - (reply: 7)
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Total protein profile - (reply: 2)
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Determining if a protein is secreted - (reply: 12)
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How to find proteins that bind to a certain motif - (reply: 1)
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Protein denaturation and renaturation - (reply: 2)
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Protein in soluble form or not - (reply: 1)
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His Tag protein purification - (reply: 3)
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Protein Extraction - (reply: 6)
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Protein deglycosylation - (reply: 1)
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Protein purification problem - protein purification problem (reply: 1)
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How to separate protein from nucleic acids? - (reply: 5)
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why can't I get GST-fusion protein? - (reply: 2)
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Storing proteins - (reply: 2)
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Protein dosage after sucrose gradient? - (reply: 2)
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prediction of protein phosphorylation sites, how? - (reply: 4)
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Storage of GST proteins in crude lysates - (reply: 1)
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problem with the size of protein - (reply: 4)
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iNOS and nNOS protein quantification from rat tissue homogenate - (reply: 1)
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Western after Ip- no IP protein, but .... - (reply: 1)
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dmp coupling to Protein G beads - (reply: 4)
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immunoprecipitation of nuclear protein - (reply: 2)
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His-tagged proteins - bacteria or mammalian systems? - Which is better and why? (reply: 1)
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Cell line to be used for protein expression an purification - (reply: 8)
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Western Blot - membrane-bound protein boiling step - (reply: 1)
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Protein expression problem - coexpression? (reply: 1)
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Protein Full Names - (reply: 3)
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Human IgA column - (reply: 1)
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protein samples on ice - how long is too long? (reply: 3)
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His tag protein purification problem - Binding of undesired protein (reply: 6)
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Quantification of heme proteins? - How to? (reply: 2)
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protein diffusion in sds page gel - (reply: 2)
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Removal of lipids from protein samples - Supernatant protein extraction with acetone (reply: 1)
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Protein disappeared after TCA precipitation - (reply: 7)
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How to extract and purify plant proteins? - (reply: 1)
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Plant protein extraction and purification - how to extract plant proteins and purify them? (reply: 1)
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making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
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Help:Total protein extraction from soil - (reply: 2)
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Lysing hela cells - poor protein recovery (reply: 17)
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low molecular weight proteins on SDS-PAGE - (reply: 1)
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IP - How much protein needed? - (reply: 3)
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Using ECF for protein detection? - ECF instead of ECL? (reply: 1)
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Problem extracting protein from plant leaf - (reply: 1)
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phosphorylated protein sequences with <20% homology - (reply: 5)
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Problems with protein expression - Protein expression, Antibody production (reply: 2)
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Big size proteins in SDS-PAGE - (reply: 3)
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protein ladder ... can be seen using ECL detection - (reply: 6)
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anyone working in protein aggregation..? - (reply: 6)
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polyclonal antibody doesn't pick up my protein during western!? - (reply: 4)
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extracellular domain from a transmembrane protein, secreted or not? - (reply: 2)
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Protein Lysate and blotting problems - (reply: 2)
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How to make an antibody against my protein - (reply: 4)
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Western blotting without transfering protein to membrane - (reply: 4)
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eluting protein from the gel band... - (reply: 7)
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aggregation of purified recombinant protein on dialysis-what do to? - (reply: 1)
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transgenic mouse analysis - I have generated a transgenic mouse for a protein that is expressed in the brain (reply: 2)
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so my protein has degraded....why am I seeing higher bands? - (reply: 3)
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Protein that doesn't express - (reply: 2)
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so tired of this protein... - another strain of E-coli? (reply: 33)
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Problems with protein extraction with Trizol - (reply: 1)
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(HELP)Properties of protein after glycosylation - any effect on pI n etc? (reply: 2)
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What is the name of substances that covalently binds proteins - (reply: 1)
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method of making nuclear extract for protein interaction? - (reply: 1)
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protein/enzyme activity asssay - (reply: 1)
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IgG concentration in normal rabbit serum - (reply: 4)
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Protein Half-life determination - (reply: 1)
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IP and appropriate control IgG - (reply: 2)
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problems with expression of mutant protein - (reply: 1)
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Western Blot - fixation of proteins with glutaraldehyde? - (reply: 4)
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TCA vs. meth/chloro - concentration of proteins (reply: 3)
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WB Loading control for large protein - (reply: 1)
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Protein migration in SDS gel - why? (reply: 2)
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A-beta 42 protein detection - a-beta 42 (reply: 5)
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crosslink protein complex in vivo - (reply: 1)
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Western Blot on an IEF separated recombinant protein - (reply: 6)
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problems whit protein solubility after purification - (reply: 5)
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Concentration of total protein lysate - column? (reply: 8)
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What can cause a change in protein level? - (reply: 1)
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Plant protein quantitation - from crude extract (reply: 2)
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protein concentration - (reply: 1)
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CHIP and CHIP with tagged proteins - (reply: 1)
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protein complex purification, what would affect? - (reply: 3)
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Western blot for two proteins: any alternative to stripping? - (reply: 11)
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protein extraction from tissue - (reply: 1)
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3D structure of a protein in X-ray crystallography - (reply: 2)
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protein isolation from brain material - yield? (reply: 8)
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Question about making low percent SDS-PAGE gels for big protein - (reply: 9)
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Which kind of post-translation modification can make protein size much bigger (3 - (reply: 8)
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phosphorylation analysis - (reply: 1)
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problems in concentration protein solution - (reply: 8)
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What is the best assay for protein interaction analysis? - (reply: 1)
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Help! Protein extract degradation ? - (reply: 1)
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is there any problem if the fusion protein was used directly as the antigen? - fusion protein was used directly as the antigen (reply: 4)
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what is the molecular weight of fusion protein in pET-32a - molecular weight of the fusion protein in pET-32a (reply: 3)
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what makes a protein insoluble? - (reply: 3)
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Concentration of secreted protein - ...step before IP.... (reply: 1)
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Pellet the proteins!? - (reply: 4)
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Protein determination in a insoluble pellet - (reply: 6)
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Protein purification wash problem - (reply: 1)
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protein contamination in DNA electrophoresis - (reply: 2)
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protein storage following purification - (reply: 1)
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Quick help on determining protein size - (reply: 3)
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protein elution from PAA gels - (reply: 2)
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Problem in protein preparation in 2-D electrophoresis - (reply: 1)
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Stabilization of protein without glycerol - protein has to be ip injected in mice (reply: 1)
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Stabilization of protein for injection in mice - when storing protein in freezer (reply: 2)
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special band in BL21(DE3) - protein expression (reply: 8)
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How to Draw a 3D structure of a Protein from its amino acid sequece? - (reply: 4)
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Epitope tags - What is T7 tag? (reply: 1)
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Anybody that has used Microcon from Millipore to concentrate protein!? - (reply: 8)
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stability of protein sample after in-gel digestion - (reply: 2)
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protein purification - (reply: 5)
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mechanism of cGMP dependent protein kinase - (reply: 2)
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How to calculate protein size in Kilodaltons - (reply: 6)
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How to sterilize a protein without denaturating it? - (reply: 2)
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problem with bacteriocin protein purification - (reply: 2)
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How much dialysis solution? - (reply: 1)
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protein precipitation / RNA binding factors - (reply: 3)
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Protein estimation - (reply: 7)
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protein dialysis - i think i just cant understand it (reply: 15)
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for protein interaction what lysis buffer I should use? - (reply: 1)
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at which step can I store the protein? - purification from inclusion body... (reply: 3)
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GST protein purification from inclusion bodies... - will GST part still be intact? (reply: 2)
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Phosphorylated proteins - (reply: 1)
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Protein behavior at high concentration - (reply: 4)
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How can I remove the Polyhistindine tag or myc epitope from my recombinant prote - (reply: 3)
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His tagged protein from mammalian cells - purification of His tagged (reply: 1)
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RNA binding protein isolation - how to figure out what binds your mRNA? (reply: 1)
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Problem with protein dialysis - (reply: 5)
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cloning membrane protein with 2 tags - (reply: 2)
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protein tranfer from gel to membrane, reversible ? - (reply: 2)
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purification problem. - most of my protein is washed away.... (reply: 10)
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SDS-PAGE for A-beta proteins from cell lysate - (reply: 6)
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protein expression - (reply: 3)
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Supershift problem (EMSA problem) - I have a problem with supershifting my protein of interest (reply: 10)
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why dose imidazole can elute protein from Ni-column - (reply: 3)
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Can I extract nuclear proteins in transfected cells? - (reply: 2)
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Outer membrane protein in the supernatant!? - (reply: 1)
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About protein expression - (reply: 1)
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WHat's the difference between Protein A and G? - (reply: 2)
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protein band - (reply: 6)
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Denature proteins on the membrane - (reply: 4)
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protein fragment on SDS-PAGE less than 3 KDa.... - how to detect it? (reply: 8)
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loading protein onto SDS-PAGE gel - (reply: 2)
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How to know if a protein that has autokinase activity is a kinase? - (reply: 2)
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Protein precipitation using Methanol/Chloroform - (reply: 1)
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Differentiating two proteins? - From the bovine potuitary glands. (reply: 2)
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Protein yeast extraction for Western blot - (reply: 3)
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Purification of His-tagget protein from inclusion bodies - (reply: 12)
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Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
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Problem in protein expression induction - (reply: 7)
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Dialysing the proteins - (reply: 3)
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Is membrane protein always insoluble? - (reply: 2)
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which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
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Immunoprecipitation of pathogen proteins with Human serum? - (reply: 1)
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western protein ladder - (reply: 9)
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Protein and Proteomics - Point mutation and protein stability (reply: 2)
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How much Antibody did I get? - Protein G column (reply: 1)
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How to increase protein expression level in cell lines - (reply: 2)
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suggest protein purification methods? - (reply: 2)
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physicochemical characterization of proteins - (reply: 5)
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phosphorylation analysis - (reply: 2)
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protein was still maitained in pellet after RIPA lysis? - in IP (reply: 4)
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Please recommend a fast way to get purified protein - (reply: 2)
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protein was still maitained in pellet after RIPA lysis - IP (reply: 3)
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hesitation between Rasmol and protein explorer - software (reply: 2)
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Sonication for protein extraction - for how long? - (reply: 11)
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DNase I......how much to use in units? - protein purification issues (reply: 1)
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Proteins that are not affected by SDS - (reply: 2)
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protein extraction from stable mammalian clones - (reply: 8)
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MM yeast two hybrid - bradford measuring of yeast proteins - (reply: 3)
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Stains for proteins.. - Silver v/s Coomassie (reply: 1)
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Help to separate fungal proteins - (reply: 9)
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Need help in Using BLAST for beginner - finding the paralog, ortholog and protein (reply: 1)
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Multiple unspecifc bands in IP using Protein A sepharose + silver staining - (reply: 2)
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protein purification - (reply: 1)
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protein concentration - (reply: 6)
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Membrane protein purification - (reply: 1)
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how to identify proteins on MS? - (reply: 1)
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question about antibodies & protein G/A sepharose in CO-IP - (reply: 6)
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protein digestion - (reply: 1)
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native gel -- protein migration - (reply: 1)
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Immunprecipitation of phosphorylated proteins - (reply: 1)
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Measure OD600 when perform the protein expression - (reply: 6)
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Protein is in insoluble fraction during overexpression in E. coli - (reply: 9)
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Differences Between Hormones - difference between the action of protein and steroid hormones (reply: 1)
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Crystallization or NMR - which is better for protein structure - (reply: 3)
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Smearing of protein samples from whole tissue lysates - (reply: 6)
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A protein whose pI is 8.9 - SDS PAGE (reply: 4)
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Protein Concentration Methods - (reply: 7)
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What characteristics are needed for a protein to be a component of immune system - (reply: 4)
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Protein transfer, high resistance causing loss in voltage? - (reply: 2)
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Effect of radiation in hypoxic and acidic cells - how to initiate the experiment about protein expression in hypoxic and acidic ca (reply: 2)
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Do repeated freeze-thaw cycles affect protein activity? - (reply: 4)
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Immunoprecipitation of protein complexes - My preciousss protein complex (reply: 3)
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How to analysis glycated proteins on SDS-PAGE - (reply: 1)
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How do proteins generally break down? - (reply: 1)
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General equation for protein degradation - (reply: 1)
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different colonies produce different amount of protein... - (reply: 6)
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His-tag fusion protein detection problem - (reply: 2)
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DNA OR NUCLEOTIDE CONTAMINATION OF PROTEIN - I NEED TO GET THIS FRAGEMENTED DNA OUT OF MY SMALL PROTEIN (reply: 4)
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Separating DNA and nucleotides from protein - (reply: 4)
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How often do your proteins have a contamination of endotoxin - (reply: 3)
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many bands other than my protein on western... - please help me to explain this... (reply: 5)
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Sequential IP's before mass spec analysis - searching for co-ip proteins (reply: 4)
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protein precipitation for mouse liver - (reply: 1)
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Transfection into a cell line which already express your protein - (reply: 3)
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bee venom peptides, proteins, enzymes - (reply: 1)
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ATP-Independent Protein Degradation ? Pls help - (reply: 4)
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Protein elution question - (reply: 1)
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how to increase protein expression in E coli? - (reply: 6)
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Protein purification from E Coli - (reply: 11)
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protein precipitation during gel loading - (reply: 3)
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membrane protein western blot - (reply: 2)
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max amount of protein for biorad combs - (reply: 2)
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Western/Signaling Studies - How to get rid of background phosphorylation? - Western/Signaling Studies - How to get rid of background phosphorylation? (reply: 3)
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westernblot problem! - membrane protein! (reply: 2)
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big black spot in the place of my protein on western... - (reply: 4)
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BCA versus BioRad protein assay - What are the advantages of BCA that all the world uses it? (reply: 3)
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PVDF versus nitrocellulose - which is better for simple Western? - "normal" protein of 13kDa (reply: 4)
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About protein extracted from liquid medium - (reply: 1)
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Protein degradation - (reply: 1)
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have my protein coagulated at -20? - very strange thing happening... (reply: 2)
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extract protein from SDS PAGE - (reply: 1)
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western blot problem - antibodies can't detect the protein on the membrane (reply: 3)
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detection of phosphorylated proteins on WB - how stable is phosphorylation once transferred on WB membrane? (reply: 6)
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opinion - denatured protein purification (reply: 1)
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Nuclear protein in a post nuclear supernatant? - (reply: 6)
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after expressing the protein can I store it for some days in -20 before purifica - (reply: 7)
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How to stop protein from preciptating during dialysis - (reply: 4)
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Gel shift assay for non purified protein preps - (reply: 1)
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Phenol chloroform extraction DNA: protein contamination - how to remove all protein from my DNA? (reply: 1)
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non IgG secreting hybridomas - (reply: 7)
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Liver tissue lysis for ER protein Western blotting - (reply: 2)
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protein purification - preserve pellet (reply: 2)
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Calcium homeostasis and protein - relationship between calcium homeostasis and some proteins? (reply: 3)
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Preparing protein Mass Spec samples - (reply: 1)
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38kda protein appearing as smear on western - (reply: 5)
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How to stain native proteins on PVDF before immunoblotting - (reply: 3)
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sds page of protein in tfa & acotonitrile - (reply: 1)
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Protein don't migrate in SDS PAGE - my protein is wierd!! help!! (reply: 1)
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protein glycosylation analysis - (reply: 3)
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any software can calculate the charge of protein - (reply: 8)
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Protein purification - (reply: 3)
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Protein imputities in extracted genomic DNA - (reply: 1)
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Looking for genomic targets of a DNA binding protein. - (reply: 1)
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Questions about proteins Please Help! - (reply: 3)
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Imidazole in column elution - Removing Imadazole from purified protein without dialysis (reply: 1)
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Cell lysis before measuring total protein content - Which method for lysis can be combined with the Bradford method? (reply: 4)
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How to get DNA binding protein in the clear supernatants? - (reply: 8)
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Trouble with S35 labeling of exogenously expressed proteins - (reply: 1)
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Expected protein size ? - fusion protein-size in kDa? (reply: 8)
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Amplification of 3KB protein from Hela mRNA PCR PROBLEM - PCR Amplification of 3KB protein (reply: 6)
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problem transfering small proteins - (reply: 4)
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denaturating protein electrophoresis - (reply: 5)
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any idea about running small size of protein? - (reply: 6)
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Question about protein expression induction - (reply: 2)
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DNA amount for protein expression - protein His-tag (reply: 1)
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Detecting proteins in Mouse embryonic fibroblast - Any tips on making cell lysates of MEFs? (reply: 2)
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Knockdown protein expression using rnai - (reply: 4)
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NIH3T3 protein expression - it isn't working (reply: 1)
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SIZE OF PHOSPHORYLATED PROTEINS - (reply: 6)
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Dephosphorylation of protein in tissue lysate - going insane,please help! (reply: 1)
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Blotting time and voltage for a 20kd protein - (reply: 4)
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Gel Filtration/Protein Precipitation - (reply: 1)
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unwanted protein degradation - question (reply: 1)
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Transfer high molecular weight proteins - (reply: 4)
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How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
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extraction of protein from aluminium hydroxide formulated vaccine - extraction of protein (reply: 1)
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protein loss in sterile filtration - suggest the 0.22 micron filter (reply: 4)
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Determine protein content of two antigens simultaneously by BCA method? - (reply: 1)
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isolation of protein from blood - isolation of protein from blood (reply: 1)
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Problem of protein expressio in different medium - help! (reply: 1)
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Porblem using streptavidin coated beads - Using streptavidin coated beads to purify proteins tagged with a biotinylated ol (reply: 2)
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protein assay inquiry - (reply: 1)
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protein expression in mammalian cell - (reply: 1)
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proteins to raise the antibody againest to HIV - proteins (reply: 1)
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How to seperate protein and carbohydrate from a mixture - (reply: 6)
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Western loading control for secreted protein? - (reply: 3)
-
[32] phosphorylation hints needed! - (reply: 1)
-
finding human homologues of 150 yeast proteins? - what database is useful for this? (reply: 1)
-
protein loss during nickle clolumn purification - is there always a lot loss of protein during column purification? (reply: 2)
-
Non-isotope detection of mRNA and protein interaction - (reply: 3)
-
Shipment of extracted proteins - (reply: 3)
-
Western blot internal controls -- Any large proteins? - (reply: 3)
-
membrane protein extraction from dissected thymus - (reply: 1)
-
protein qantification and concentration - (reply: 5)
-
How to calculate the size of a protein (in kDa)? - (reply: 1)
-
Large protein expression in E.coli - (reply: 5)
-
Protein extraction - (reply: 1)
-
immunoprecipitation of membrane protein from gram+ bacteria - (reply: 2)
-
protein ppt using acetone - (reply: 1)
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No expression of his-tagged protein - (reply: 4)
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Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
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Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy (reply: 3)
-
What is the best way to identify a protein isoform? - (reply: 4)
-
problem purifying a his-tag protein - (reply: 2)
-
need ideas for protein protein interaction - protein interaction assays (reply: 3)
-
siRNA causing mRNA knockdown but no protein knockdown! - (reply: 4)
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way to confirm that my protein is active - (reply: 4)
-
Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
-
Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
-
How to remove LPS from protein solution - (reply: 2)
-
protein synthesis - (reply: 2)
-
Using TBST buffer - protein phosphorylation (reply: 3)
-
How amido black stains protein? - (reply: 1)
-
DH5 Alpha protein expression - (reply: 1)
-
GST protein purification - only 3% bind to beads (reply: 6)
-
Figures of proteins - Figures of proteins (reply: 7)
-
Proteins expression-temperature inducible vs IPTG inducible system - (reply: 2)
-
protein can be found by IHC but not WB - (reply: 2)
-
why get rid of imidazole in his tag protein purification - (reply: 3)
-
small sized protein tailing off - (reply: 1)
-
Protein expresses wrong size - (reply: 5)
-
Expression interference from His-tag - transcription protein (reply: 2)
-
What is the best nuclear protein marker? - Fractionation (reply: 3)
-
lysis is not translucent after urea treatment - need help with His tag fusion protein purification (reply: 2)
-
renilla Luciferase and protein concentration - (reply: 1)
-
Tips for Western Blot of Low Molecular Weight Protein - (reply: 4)
-
Protein sequence comparison report - (reply: 2)
-
Harvesting of cells before fractionation - escape of proteins? (reply: 3)
-
Help RNA and protein extraction - i dont understand the chemistry behind it (reply: 3)
-
DNA, RNA and protein extraction using basic solution - (reply: 4)
-
his tag protein not expressing - I am stuck !!! (reply: 6)
-
TriReagent for protein isolation? - (reply: 3)
-
6 His tagged protein purification - (reply: 1)
-
Phosphorylation of TP53 - (reply: 3)
-
PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
-
Removing salt and immidazole from purified Protein - (reply: 2)
-
Trouble expressing a protein in transient transfection - (reply: 2)
-
Do you know a commercial protein which attaches well to Vero cells - Need a positive control in immunofluorescence (reply: 1)
-
Problem resuspending precipitated protein - (reply: 2)
-
Freezing a Pellett.. (or however its spelt) - Spin laballed over expressed protein (reply: 2)
-
How to prevent the protein degradation - protein degradation (reply: 2)
-
Measuring protein concentration - (reply: 3)
-
loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
-
overexpress his-tag protein, wrong size?! - (reply: 1)
-
trouble in scaling-up protein expression - (reply: 5)
-
membrane protein - (reply: 1)
-
How to measure labelled protein concentration? - (reply: 1)
-
Histone like protein extraction from Gram positive - (reply: 1)
-
His-tag protein purification - (reply: 1)
-
Removing Triton X-100 without denaturing the protein? - (reply: 6)
-
Human IgG from protein G - (reply: 1)
-
why glycerol used in protein purification - (reply: 1)
-
Same protein, different size on Western and IP ? - (reply: 2)
-
degradation of cloned protein in E. coli - (reply: 2)
-
imidazole in protein purification - (reply: 2)
-
Path length measuring protein concentration - (reply: 3)
-
Western transfer time: Is it crucial for big proteins? - anomaly HELP (reply: 2)
-
protein expression ! - please help me with this.. (reply: 1)
-
protein dephosphorylation by alkaline phosphatase? - (reply: 8)
-
Protein Purification - Advice - dialysis/desalting/concnetrating and antibody production? (reply: 1)
-
problem in expression of his tag protein - (reply: 6)
-
protein extraction - (reply: 5)
-
Strange protein transfer - (reply: 3)
-
Protein fluorescence - (reply: 7)
-
IP the membrane protein - How to elute the protein complex from the beads (reply: 2)
-
is this protein too toxic to bacteria? - (reply: 5)
-
anti-c-myc-agarose protein purification - (reply: 1)
-
protein isolation - cell pellet (reply: 4)
-
Phosphorylation with PNK - (reply: 5)
-
Regulation of protein may not be detected at RNA level - Can anyone tell me why??? (reply: 3)
-
pure protein - .. (reply: 4)
-
Protein detection without using photographic film - (reply: 5)
-
protein extraction from leaf - (reply: 4)
-
What's the meaning of heterologous protein? - (reply: 1)
-
Quantify protein expression - (reply: 1)
-
Weak Western band for phosphorylated Akt - whats happening?? (reply: 17)
-
which tag and vector is the best for expressing protein in bacteria? - (reply: 4)
-
western blots and big proteins - (reply: 7)
-
CHIP with Tagged Proteins - (reply: 2)
-
Removal of nucleic acid from protein extract? - (reply: 1)
-
question about natively seperation of subunits of complex protein by PAGE - (reply: 1)
-
Anyone know of free-use Fluorescent proteins? - (reply: 12)
-
How to quantify fluorescent-tagged protein - (reply: 2)
-
Nuclear translocation - nuclear transport of proteins without NLS (reply: 1)
-
help - small proteins and western blotting - (reply: 23)
-
Basal protein expresion in pRSET a - (reply: 2)
-
protein extraction efficiency - protein extraction efficiency (reply: 3)
-
protein expression - (reply: 3)
-
Loss of proteins during Western transfer - (reply: 4)
-
HOW TO prevent protein degradation - (reply: 7)
-
Aggregation of protein after purification? - (reply: 2)
-
Which companies sell protein arrays? - (reply: 3)
-
protein isolation of plant tissue -trizol regent - (reply: 3)
-
GST fusion protein extraction - (reply: 5)
-
gene expression vs protein expression - (reply: 3)
-
how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
-
Protein analysis of conditioned media - How to prepare media before SDS-PAGE? (reply: 4)
-
No transfer of protein on PVDF membrane - Western blot troubleshooting (reply: 5)
-
Phosphorylation - (reply: 8)
-
Where did the protein go? - Low protein reading in WB lysates (reply: 4)
-
How to detect receptor on the cell surface? - using the less viral protein possible (reply: 1)
-
disulfide bonds in proteins - (reply: 4)
-
codon bias? - protein expression (reply: 11)
-
Silly question on His-tagged protein - (reply: 2)
-
Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
-
Purification of enzymatically active protein from PAGE - (reply: 9)
-
Protein rofolding problem - percipitation (reply: 4)
-
Testing for binding of mutant proteins to cell surface receptors - (reply: 3)
-
sonication free protein extraction... - (reply: 2)
-
Factors that may influence recombinant protein expression - (reply: 9)
-
Effect of APS (Ammonium persulfate) to protein - (reply: 1)
-
Protein precipitation problems - (reply: 3)
-
protein did not enter the gel on EMSA - (reply: 2)
-
Advice on Protein expression Services - (reply: 4)
-
6 his tagged protein purification - purification (reply: 2)
-
protein purification - (reply: 1)
-
ELISA with denaturated proteins? - (reply: 6)
-
protein free medium - (reply: 1)
-
Expressing GST-Fusion protein - Are larger protein inserts harder to express? (reply: 4)
-
protein/enzyme activity - lost activity after his tagging (reply: 1)
-
Protein samples still in liquid form at -20C - Storage condition for proteins (reply: 8)
-
Large scale protein purification - Yellow and cyan fluorescent protein (reply: 4)
-
protein purification - (reply: 3)
-
protein expression protein - molecular biology (reply: 6)
-
Does imidazole absorbe at 280 nm? - How do you measure your protein content? (reply: 4)
-
Troubleshooting flag-tagged secreting protein expression - Troubleshooting, Improvement, tricks! (reply: 5)
-
the range of protein amount for loading - western blotting (reply: 4)
-
Protein measuring - (reply: 3)
-
optimal SDS separation protein - (reply: 3)
-
Help!recombinant Protein degradation during purification - Proteolysis (reply: 1)
-
phosphorylated oligos and vector - (reply: 2)
-
extraction of membrane protiens - protocol for extraction of membrane bound protein? (reply: 6)
-
Protein Structure Prediction - HELP!! (reply: 4)
-
sterilizing protein solutions + agar - how to sterilize sensitive media (reply: 4)
-
PQE vector - Getting protein in its soluble form (reply: 3)
-
protein dialysis - a couple of impt, urgent qns (reply: 3)
-
sample preparation for IEF - trouble in normalizing protein (reply: 1)
-
What is a prenylated protein? - (reply: 2)
-
Purifying a membrane protein - - the trouble with lipids? (reply: 3)
-
GST-recomb-fusion-protein antibody - antibody against gst-fusion protein (reply: 1)
-
determining protein expression level from a Western!!! - (reply: 6)
-
ELISA beginner - bacterial protein (reply: 2)
-
Help with concentrating proteins in culture medium - (reply: 4)
-
false results with the BCA assay? - (reply: 4)
-
WB- proteins of lower-than-expected MW - (reply: 1)
-
Identification of phosphorylation site - help (reply: 1)
-
Making solutions for protein prep - (reply: 3)
-
Myc-tag in ChIP - problems with ChIpping myc-tagged proteins (reply: 1)
-
shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
-
High protein expression but low protein activity - MBP-tagged fusion protein inactive (reply: 2)
-
protein sizes - which tools (reply: 1)
-
Same function for a specific protein in any locations? - protein function (reply: 2)
-
How much a protein converted into a crystal? - crystallization (reply: 2)
-
Is 100 ug liver tissue protein lysate too much for western blot? - (reply: 2)
-
How to make pre-stained protein marker in lab - (reply: 1)
-
Purification of proteins from human tissue - SDS_PAGE (reply: 1)
-
How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein (reply: 3)
-
Problem in using EMSA to assay protein fractions - (reply: 1)
-
Yeast Fluorescent Proteins - Yeast Fluorescent Proteins (reply: 1)
-
No protein expression after IPTG induction - No protein expression after IPTG induction (reply: 6)
-
What is the role of integral membrane proteins in signaling? - (reply: 1)
-
my protein does not freeze, help - (reply: 11)
-
brain protein isolation for WIB - can I simply use 4xLB+BME? (reply: 2)
-
protein isolation from brain - (reply: 1)
-
GFP protein not soluble? - (reply: 1)
-
small protein expression in yeast - expression of an 8kD his tagged protein in yeast (reply: 1)
-
Protein hydrophobicity - (reply: 1)
-
WB for Membrane Protein - Not able to see membrane protein on western (reply: 12)
-
Expressing protein in a soluble form - What should be the next logical step? (reply: 8)
-
anyone has idea to raise antibody, how much protein need? - (reply: 1)
-
Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
-
His-tag protein purificaton, cotamination with 40-43kD protein - (reply: 1)
-
MBP tagged protein not bind resin - (reply: 8)
-
Protein Expression Analysis Question - Protein Expression Analysis (reply: 1)
-
cDNA size VS protein Size - MW weight confusion (reply: 7)
-
Protein Solubilisation - Dissolving tofu (reply: 4)
-
Acetyl coenzyme A and dialysis - Removing AcCoA and dialysis problem (reply: 2)
-
Western blot of phosphoproteins - (reply: 2)
-
Protein Expression - Question (reply: 1)
-
denature a protein of interest - (reply: 4)
-
aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
-
Co IP with myc and ha tagged fusion proteins - (reply: 7)
-
Protein interaction - (reply: 3)
-
protein expression level optimization - general question (reply: 1)
-
Stability of protein in powdered leaf sample - Protein stability after quick freeze in liquid N2 (reply: 1)
-
problem with Protein concentration determination and westernblot - (reply: 11)
-
Protein not penetrating the resolving part of the gel - (reply: 2)
-
BCA assay - folded or denatured protein (reply: 2)
-
Does SUMO fusion protein increase solubility? - (reply: 2)
-
FITC Conjugation of proteins - (reply: 1)
-
<10kD protein in Western - (reply: 3)
-
How much an H-bond can contribute to protein intergrity? - Protein structure & hydrogen bonds (reply: 2)
-
Mitochondrial, cytosolic and Nuclear Protein extractions - An all in one protocol? (reply: 1)
-
Immunofluorescence - cell surface protein detection - How do I detect only cell surface expressed prot. (reply: 3)
-
whether someone know about such a kind of proteins - (reply: 1)
-
2D identified proteins - (reply: 2)
-
Proteins and marker are not running down gel - Protein purification (reply: 7)
-
plant protein purification - pectin removal from crude extract (reply: 1)
-
prepartion of membrane protein - (reply: 3)
-
Methods to measure a desired protein's half-life? - (reply: 3)
-
About pH and pI for his-tagged protein purification - (reply: 2)
-
No change in mRNA or protein levels after RNAi in Trypanosoma brucei - (reply: 4)
-
Homemade DNA/Protein A beads - i think i made a boo-boo (reply: 2)
-
No expression of 7kDa His-tagged protein - why? - Poor protein expression (reply: 9)
-
Bacterial cell wall protein refolding problem - protein refolding (reply: 4)
-
no protein expression after induction using pET vector - (reply: 4)
-
Methods of proteins extraction from plants - protein extraction industry (reply: 1)
-
Protein pellet solubilization trouble - (reply: 2)
-
glycerol is still there after dialysis - (reply: 1)
-
Protein extraction from annatto fruit tissues - (reply: 3)
-
Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
-
dialysis of purified protein - (reply: 5)
-
Protein isolation for western - (reply: 8)
-
His-tagged protein co-eluted with associated protein - (reply: 4)
-
PROBLEM: Western blot of a 220 kd nuclear protein - (reply: 3)
-
extraction of extracellular protein of C. neoformans - extraction method (reply: 3)
-
Actin Polymerization Assay...Anyone? - Native or Denatured Protein (reply: 1)
-
protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
-
Can antibodies and proteins be vortexed? - (reply: 4)
-
protein A tagged fusion protein - (reply: 1)
-
aggregates in SDS-PAGE? - double mw of proteins in SDS-page (reply: 2)
-
Temperature for protein expression - (reply: 2)
-
in-vivo method of identifying 2 protein binding? - (reply: 3)
-
MW of protein on SDS-PAGE different from actual mass - (reply: 4)
-
quantify protein on beads? - (reply: 4)
-
difficult to dissolve protein? - (reply: 1)
-
"Help" : how to identify of proteins binds to specific DNA - (reply: 3)
-
Concentrating protein in cerebrospinal fluid for Western - (reply: 1)
-
Proposal for strategy to determine 2 protein interactions? - suggest sugges! (reply: 11)
-
express a big protein in BL21, get an extra band - (reply: 7)
-
western of a big protein - (reply: 2)
-
His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
-
Insect cell sonication protocol? - Protein Purification (reply: 1)
-
amount of protein to load - (reply: 4)
-
Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
-
can i store gel cut for dialysis - (reply: 3)
-
How to solve a protein when not soluble in 6M GuHCl? - (reply: 1)
-
signal peptide for the vesicular proteins - (reply: 1)
-
GST proteins detection - (reply: 1)
-
protein yield - (reply: 2)
-
A question about cell stain: fix the cell have fusion protein - (reply: 1)
-
having hard time to re solubilize after protein aceton ppt - protein precipitation (reply: 6)
-
Help on protein integrity Western Blot - Sample preparation and detection (reply: 6)
-
Western blotting of small proteins - (reply: 3)
-
how quick the protein get degraded in room temp - (reply: 6)
-
Concentration of secreted protein in culture media - Concentration of culture media (reply: 7)
-
DNA "clean-up" - How to get rid of DNA contaminants: RNA, proteins (reply: 1)
-
IP - losing proteins from protein G beads - (reply: 1)
-
STAT 1 / phospho STAT 1 - can't detect my protein (reply: 3)
-
phosphorylation experiment without using radiation? - (reply: 8)
-
Normalizing protein expression under 24hrs ischemia - normalization with housekeeping genes (reply: 5)
-
Protein extraction from solid matrix - (reply: 1)
-
Protein sticking to membrane - Protein concentration woes (reply: 1)
-
expression of fusion protein - how to lysis membrane protein (reply: 2)
-
His-tagged protein binding problem - Protein Binding (reply: 1)
-
Size Exclusion Column - Proteins keep sticking!! - (reply: 2)
-
a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
-
Western Blotting - Electrophoretic transfer of proteins (reply: 2)
-
problem with DNA extraction - can't get rid of protein phase (reply: 3)
-
membrane and mitochondrial protein fractionation - (reply: 1)
-
BCA assay - (reply: 2)
-
separate proteins from sucrose - (reply: 3)
-
Protein consensus pattern (motif) database - (reply: 1)
-
Protein consensus pattern (motif) database - (reply: 4)
-
IgG's "epsilon" - (reply: 2)
-
Expression of N-terminus fusion protein problem - (reply: 1)
-
protein quantification- triton - (reply: 3)
-
how should I concentrate my protein?? - (reply: 12)
-
phosphorylation - (reply: 5)
-
protein arginine methylation in E. coli? - (reply: 2)
-
what generally methods to improve the protein solubility - (reply: 1)
-
MBP tagged recombinant protein - (reply: 1)
-
solubility of protein - (reply: 1)
-
no proteins being made?? - (reply: 2)
-
NP40 concentration in protein lysis buffer - (reply: 1)
-
IP of endogenous protein - (reply: 1)
-
help to see whether my protein is imported to the nucleus - please give some advice (reply: 5)
-
problem whether a protein is located to the nucleus - please give some advice (reply: 3)
-
protein quantitation - for equal western loading (reply: 2)
-
there seems no induction of my recombinant protein - (reply: 4)
-
Protein Homogenization - Soluble and insoluble protein (reply: 3)
-
detection of protein phosphorylation using M.S. - (reply: 4)
-
Protein produced with brown color? - I have some problem in protein expression (reply: 1)
-
can I freeze the gel slice for protein sequencing - (reply: 1)
-
His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
-
question about the protein extraction - (reply: 1)
-
recombinant protein lost immunological activity - (reply: 1)
-
Storing a protein membrane overnight? - (reply: 5)
-
more questions about protein expression... - (reply: 1)
-
How to identify a protein that binds a defined DNA sequence - (reply: 4)
-
Express a vaccine protein - (reply: 1)
-
Trouble with Western Blot Transfer (Poor transfer) - Could the protein isolation or homogenization be the cause? (reply: 4)
-
Ni-NTA problem - His-tag protein that never bound with Ni-NTA agarose (reply: 10)
-
His-tag protein purification - Question (reply: 9)
-
Protein with His6 tag - (reply: 2)
-
Need protocol for SDS-Page on protein extracted from soil - (reply: 1)
-
Nuclear protein extract (Scrape or Trypsinize)? - (reply: 1)
-
SDS-PAGE of protein from membrane fraction - problems with protein band profile (reply: 1)
-
Protein degradation - Recombinant protein degradation in insect cells (reply: 1)
-
IgA anti Ovalbumin - (reply: 1)
-
isolation of nuclear protein - isolation of a nuclear protein, namely Akt protein (reply: 1)
-
IgG contamination of immunoprecipitated proteins - (reply: 1)
-
Removing Exonuclease From Protein Prep - How can I remove exonuclease from a protein prep (reply: 4)
-
protein sequence alignment using suffix tree - (reply: 1)
-
Cleaved GST fusion protein retained on beads! - Affinity of GST-cleaved protein for glutathione sepharose (reply: 7)
-
Signal peptide - periplasmic protein - (reply: 2)
-
the protein band disappear in the second dimension - 2D-Gel (reply: 2)
-
Plant cell wall protein - remove the polysaccharide and purify the protein (reply: 1)
-
antibodies to epitope tags - (reply: 1)
-
No Protein Induction - (reply: 3)
-
protein precipitation after IMAC - protein purification (reply: 1)
-
membrane protein purification - solubilization and detergent removal (reply: 2)
-
measuring protein concentration - (reply: 5)
-
NOC - A program for protein structure explorer - https://noc.ibp.ac.cn (reply: 1)
-
Solubility ribosomal proteins - Solubility ribosomal proteins (reply: 4)
-
protein expression - pkk223-3 vector (reply: 3)
-
Protein extraction from rat liver tissue - (reply: 1)
-
protein solubility - (reply: 1)
-
Protein purification - Problem with his-tag protein gel filtration (reply: 5)
-
protein dimerisation - how do I determine if it is? (reply: 1)
-
Have any one know about Protein A - (reply: 2)
-
The best way to get nuclear proteins from mammalian cells - How to break your cells? (reply: 1)
-
neomycine resistance protein antibody - does anyone exist? (reply: 2)
-
Question about silver staining - How silver saining can bind all of DNA, protein and RNA? (reply: 2)
-
Protein expression problems - (reply: 4)
-
Phosphorylation of large quantities of protein - (reply: 1)
-
Bacterial Periplasmic protein localization - B-lactamase- alkaline phosp Fusion experiments (reply: 4)
-
GST fusion protein quantitation - (reply: 1)
-
quesiton about GST fusion protein - (reply: 1)
-
TCA protein precipitation - (reply: 2)
-
How to predict the 3-D structure with the sequence of protein? - (reply: 4)
-
Is adding reducing agent necessary before protein boil? - For Western blot (reply: 4)
-
Removal of endotoxin from proteins & DNA - Protein & DNA purification (reply: 15)
-
Protein precipitated after buffer exchange - (reply: 3)
-
problem with expression of GST fusion protein - (reply: 1)
-
Protein isolation from acrylamide gels - (reply: 2)
-
protein purifiacation - (reply: 1)
-
Problem in non-specific protein synthesis - Please help! (reply: 1)
-
trizol extraction for phospho proteins - (reply: 4)
-
protein solubility in TCA - PRPs have special properties? (reply: 1)
-
aggregation of purified proteins - (reply: 4)
-
Phospho Protein detection method - Phosphoprotein detection with phospho-specific dye (reply: 1)
-
How imidazole can be washed off the Ni-NTA agarose beads - reuse of Ni-NTA agarose beads for His protein (reply: 2)
-
protocol for immunoprecipitation of bacterial proteins - (reply: 3)
-
total protein in Western blotting - (reply: 1)
-
Amersham Hybond-P membrane and small protein - (reply: 2)
-
Problem in protein expression - (reply: 5)
-
Sucrose Gradient Protocol for IgG/IgM separation? - Protocol for sucrose IgM separation (reply: 1)
-
Protein reduction without mRNA reduction? - (reply: 1)
-
GRA1 expression - elongation of the protein (reply: 1)
-
UV (280nm) Protein Quantification - Rough estimation of [protein] does it work? (reply: 1)
-
membrane protein in SDS-PAGE ! - (reply: 3)
-
let me have a simple test for detecting phosphoproteins - (reply: 5)
-
Inconsistent results on protein intereations - (reply: 1)
-
Binding of protein to immunoassay plates - binding in denaturing conditions (reply: 1)
-
tracking 150kDa protein through tissue - molecule needed for tracking (reply: 1)
-
Protein solubilization and Western Blotting - (reply: 1)
-
How to insert a Tag to the middle of a protein? - (reply: 3)
-
check protein expression from tissue - (reply: 1)
-
Membrane Protein Western Blot. - Troubleshooting western blot (reply: 4)
-
Protein Structure - (reply: 1)
-
Theory question on protein phosphorylation - (reply: 2)
-
Phosphorylation not affecting SDS-PAGE mobility - (reply: 7)
-
SDS-PAGE high MW protein trapped - 250 kDa protein is trapped at the interface (reply: 2)
-
remove BSA from IgG prep - (reply: 2)
-
How resuspend protein after (NH4)2SO4 precipitation? - (reply: 2)
-
losing protein activity - (reply: 3)
-
His-tag protein purification - contamination with other... - ...His-rich proteins (reply: 15)
-
Extraction of membrane proteins and western on them - (reply: 3)
-
Proteins in sample buffer - Interference with protein assays (reply: 1)
-
I forgot to pretreat my dialysis membrane ! - (reply: 1)
-
I forgot pretreating the dialysis membrane ! - (reply: 1)
-
Who is my closest gene relative? - Help to determine phylogeny of a protein (reply: 2)
-
Problem with his tag protein purification - (reply: 2)
-
protein purification - his-tag protein purification (reply: 2)
-
gst-fusion protein, mainly - recombinant protein (reply: 2)
-
choosing insect cell for high-cystine protein expression - (reply: 1)
-
Flag-tag protein - Need advice for Flag-tag protein (reply: 1)
-
SDS-PAGE question - seperate protein (reply: 3)
-
protein purification problem - DNA stuck to protein problem (reply: 1)
-
help with protein size - western blot (reply: 1)
-
Degradation of GST fusion proteins - (reply: 1)
-
protocols for mouse cells - splenocyte preps, protein extraction (reply: 1)
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Electroblotting of Proteins on - transfer buffer without Tris and Glycine (reply: 1)
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RNA/protein Isolation - Is there some good KIT or ...? (reply: 3)
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"disappeared" Proteins Using Bradford - Bradford reaction does not give correct results (reply: 2)
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problems with loss of purified protein due to precipitation - protein purification (reply: 4)
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Expression of Proteins for western blot - (reply: 1)
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Phosphorylation of protein - Trouble with backphospho (reply: 3)
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Phosphorylation site and Src - question about a phosphrylation site and an SH3 (reply: 1)
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protein lysates preparation - (reply: 4)
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SDS gel troubleshoot - My proteins do not show up in the gel. (reply: 1)
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Protein expression troubleshooting - (reply: 2)
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protein content in FBS - (reply: 1)
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protein expression - How to express protein of a high molecul (reply: 4)
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[35s] Met labeling protein protocol ! - (reply: 1)
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293T cells go wild after >50 passages??? - protein expression changed!?? (reply: 1)
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GST fusion protein degradation - (reply: 2)
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question about SDS-PAGE - electrophoresis of proteins (reply: 4)
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western blotting, (GFP is there - But where's the protein I want?) (reply: 3)
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Purification of nuclear proteins - (reply: 2)
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prediction of putative epitopes - reliable softwares for epitope predictio (reply: 1)
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TCA precipitation of proteins - (reply: 15)
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can RIPA buffer use to prepare nuclear protein? - (reply: 1)
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western blotting of expressed protein - (reply: 1)
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IP/Western complication - Trying to pull down 2 conjoined proteins (reply: 2)
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protein expression in Pichia pastoris - (reply: 91)
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protein aggregation - aggregation during concentration (reply: 2)
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help with protein expression and detection - (reply: 4)
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protein aggegation or? - protein in the wells of native gel (reply: 3)
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ionic Charge and pI of protein information!? - Proteomic (reply: 2)
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His tag protein purification problem - (reply: 66)
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isolate secreted proteins - (reply: 3)
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Membrane protein isolation - (reply: 1)
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Protein concentrators - (reply: 1)
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protein mistargeting - (reply: 1)
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DNA binding proteins - (reply: 3)
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Protein Domain Id program/site? - (reply: 2)
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Dot blot on bacteria - screen of protein expression (reply: 1)
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Dialysis - (reply: 2)
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western blot for membrane protein with his-tag - can't detect with anti-his tag Ab? (reply: 3)
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Concentrating proteins from solution - (reply: 2)
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FORTE protein profile¨Cprofile comparison tool - (reply: 1)
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Problem with protein concentration - (reply: 11)
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Extracting Protein Using TriZOL - Is it possible? Are there problems? (reply: 3)
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where can I predict the 3-D structure of a protein - (reply: 1)
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Triton X-100 - solubilization of proteins (reply: 1)
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Antibodies using Recombinant proteins as antigen - (reply: 3)
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Protein Expression - (reply: 1)
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protein concentration - (reply: 5)
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concentrate protein in culture media? - (reply: 2)
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Antibodies to recombinant proteins - Any advice on production of proteins? (reply: 2)
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Detection of low abundant proteins with IHC - (reply: 1)
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Help! - Method to enhance protein expression! (reply: 1)
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Protein pellet solubilisation - (reply: 4)
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protein expression - I can not express in E.coli (reply: 2)
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His purification of a regular expressed protein - (reply: 1)
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Nucleic acid contamination in protein extract - (reply: 2)
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protein precipitation - protein precipitation (reply: 5)
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protein aggregation - prevention/inhibition (reply: 4)
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Spec problems - Trying to measure protein conc. (reply: 2)
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Separating protein from sucrose by precipitation? - help! (reply: 1)
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tissus proteins extraction - (reply: 3)
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Protein Concentration from Medium - (reply: 2)
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Hydrophobic proteins extraction from yeast wall cell - How to extract hydrophobic proteins? (reply: 1)
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Purification of a cell membrane protein - (reply: 1)
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Protein extraction protocol - (reply: 1)
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Western Blot for Elastin orother Matrix Proteins - (reply: 1)
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Protein assay - Percoll containing samples (reply: 1)
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non-radioactive detection ofphosphorylation - (reply: 2)
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total protein assay in cell extracts - (reply: 3)
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protein isolation - (reply: 1)
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Yeast protein extraction - need to change my expression system... (reply: 3)
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Protein extraction from gram-positive bacteria - (reply: 1)
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Precipitation of Native Protein - IMAC of Native Protein (reply: 1)
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Protein expression with IPTG in pET3a system - (reply: 3)
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Autoradiography for proteins - Radioactive protein probes (reply: 1)
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Protein Glycosylation/Thrombin cleavage of GST fusion prote - Proteomics (reply: 2)
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protein precipitates after dialysis - Protein purification (reply: 4)
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please help me - how to extract cd3 protein from jurkat cell (reply: 1)
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PROTEIN ASSAY - LISTING ALL THE PROTOCOLS FOR ASSAYING IMPORTANT PROTEINS (reply: 2)
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prevention of protein precipitation during dialysis - (reply: 3)
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PROTEIN ASSAY - MEASUREMENT OF PROTEIN IN CELL LYSATES (reply: 2)
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How to elute proteins from a SDS-PAGE gel? - (reply: 1)
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How to extract hydrophobic proteins from yeast wall cells? - (reply: 1)
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URGENT: IgG subclass - (reply: 1)
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ELISA - direct protein assay with ELISA (reply: 1)
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protein fluorescent labeling - (reply: 1)
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Murine MAb IgG purification - monoclonal antibody purification from ascites & supernatant (reply: 1)
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Proteins and Membranes - (reply: 2)
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electroelution of protein fromsds-page for use in immunization - (reply: 2)
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Protein synthesis inhibitionassay - (reply: 2)
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IgM Purification - (reply: 1)
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IgG determination - (reply: 1)
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IgM hypermutation - (reply: 1)
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protein quantification - protein expression (reply: 1)
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about IgG - (reply: 1)
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Electroelution of protein fromSDS-PAGE for use in immunization - (reply: 3)
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Protein extraction from humantissue - (reply: 1)
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Protein Extraction fromLymphocytes - (reply: 1)
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problem with trizol - solubilization and western of proteins (reply: 2)
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protein solubilization with NDSB - (reply: 2)
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Ligation in PTYB12 vectors ofthe IMPACT protein purification system... - (reply: 1)
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His-tag protein purification - (reply: 5)
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his-tagged protein purification - (reply: 1)
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membrane protein - (reply: 2)
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protein express - (reply: 1)
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Proteins sticking to Dialysis Bag - (reply: 3)
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Sigma nuclear protein extraction - (reply: 1)
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"pSP64T" where can Iget it - (reply: 1)
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How to concentrate protein from culture medium - for Western blot (reply: 1)
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Search for a protein containingthis AA sequence - (reply: 1)
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Plant Protein Extraction - (reply: 1)
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DNA contamination in protein sample - DNA contamination (reply: 2)
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Flag Tagged Proteins - (reply: 1)
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protein linker??? - (reply: 1)
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Polysaccharides co-precipitatingwith protein in ammonium sulfate - (reply: 1)
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Nickle column and His-tagged protein purification - recharging Ni+2 column (reply: 1)
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Protein extraction form gels - (reply: 2)
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Total Protein Extraction - (reply: 1)
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electroelution - precipitate on dialysis (reply: 3)
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His tag - protein purification - (reply: 2)
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Conservation GST fusion proteins - (reply: 1)
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insulin like growth factor IIprotein - (reply: 1)
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Supernatant proteinprecipitation - (reply: 1)
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Sigma Nuclear Protein ExtractionKit - (reply: 1)
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Bradford Protein Assay - (reply: 2)
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Protein Extraction from planttissue - (reply: 1)
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Protein size ! - (reply: 6)
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Electroblotting of proteins(western) - (reply: 2)
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SDS-PAge problem! - Protein separation (reply: 5)
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Complete elution of proteinsfrom SDS-PAGE - (reply: 1)
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Protein extraction from bacterial cells - Suitable protocol (reply: 1)
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Making GST Fusion proteins - (reply: 1)
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membrane protein purification - how to pufify cd3 protein from jurkat cell (reply: 1)
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Resuspension of Triton-insoluble proteins for SDS-PAGE - (reply: 2)
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protein expression - What is a suitable strain (reply: 1)
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protein purification - (reply: 3)
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proteins electrophoresis - (reply: 2)
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Protein solubilization - (reply: 1)
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how to precipitate the nucleicacid with out proteins - (reply: 2)
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Methods needed (E.coli): - membrane separation, spheroplasts, periplasmic proteins (reply: 1)
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Hydrophobic proteins extraction? - (reply: 1)
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protein expression - (reply: 5)
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Bradford Protein Assay - (reply: 1)
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Micro BCA Help needed (Pierce);mistake made! - (reply: 1)
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crosslinking of monoclonal Absto Protein G (or A) -Sepharose - (reply: 1)
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Protein isolation using Trizol - Trizol for protein isolation and subsequent use in W-blot (reply: 5)