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Protein in active form - (Sep/06/2006 )

Hi colleagues
I want to ask whcih test is good to check protein activity. Some time you get 90 percent soluble protein but protein is not active form. So any one have a good idea about to check the activity of protein . I know little bit about pull down assay but any one knows some thing more simple or some more good test to check the protein activity.
best regards
samita

-samita-

QUOTE (samita @ Sep 6 2006, 11:59 AM)
Hi colleagues
I want to ask whcih test is good to check protein activity. Some time you get 90 percent soluble protein but protein is not active form. So any one have a good idea about to check the activity of protein . I know little bit about pull down assay but any one knows some thing more simple or some more good test to check the protein activity.
best regards
samita


Depents on the protein, for kinases we┬┤re using a kinase-assay (cook-aasay) for the activity. Every protein is a diva, therefore no general answer...

-ms-olli-

it depends, of course, on the protein: simpliest for enzymes is to check enzyme activity; binding assays to other proteins is more difficult to estimate activity; ligand binding assays are commonly used for receptors; some proteins such as membrane proteins have to be reconstituted to membrane for full activity; may be there is some literature to assay your special protein

-The Bearer-