How to get rid of contaminated nucleases from purified proteins - (Aug/08/2006 )

Hey everyone,

I have purified a protein from E. coli which will be subject to an assay which requires nuclease-free condition. And I realize that the proteins that i have purified have nuclease contamination, possibly because it's too concentrated...Could anyone give me advice and suggestions of how to clean up the nucleases? Thanks.

Ron

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If ur "purified "protein has nuclease contamination. I would suggest u repeat purification with a better accuracy method. If not possible, try repeatedly purifying ur sample till u get sufficient purity.
I cant think of any method 4 removing nucleases only.

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If there's a size difference between the protein and the nucleases, try gel filtration. Otherwise, try ion exchange.

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