islet protein extraction - (Sep/24/2008 )

hi


i am trying to extratc protein from islets cells ? i am using 500 islets in 50ul RIPA but still dont have enough concentration?

could you please help with this asap??????

thanks

Well, the obvious answer is to use more cells or less lysis buffer...It's not very helpful however.

I suggest trying to precipitate the protein and resuspending in a smaller volume.

Another suggestion is that you protein is insoluble in the RIPA buffer, try playing with the detergents used to see if that makes any difference.