Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

I accidentally put two start codons on my protein . . . - (Aug/04/2006 )

Does anybody know if I will have problems expressing this protein? Should I reengineer it to take out the second start codon?


Things to check: Are the two codon in frame to what you wish for? Is your N-terminal sequence critical to the protein that you want to express?


i would think that one would be the start codon and the other would just code for methionine.


same as mdfenko. as the positionning of ribosome is driven by more that ATG, the first codon is the starting met and the other one is an extra methionine. Moreover i don't think 2 met in begining will be a severe problem, as it's only 1 repetition.