I accidentally put two start codons on my protein . . . - (Aug/04/2006 )
Does anybody know if I will have problems expressing this protein? Should I reengineer it to take out the second start codon?
-amulekii-
Things to check: Are the two codon in frame to what you wish for? Is your N-terminal sequence critical to the protein that you want to express?
-genehunter-1-
i would think that one would be the start codon and the other would just code for methionine.
-mdfenko-
same as mdfenko. as the positionning of ribosome is driven by more that ATG, the first codon is the starting met and the other one is an extra methionine. Moreover i don't think 2 met in begining will be a severe problem, as it's only 1 repetition.
-fred_33-