Ab's production from recombinant proteins (2) - new idea (Dec/04/2007 )
I have new thought (idea) upon the information i got from u.
I am trying to produce poly Ab's. As i understood, when using danaturing protein i likely to
lose Ab's produce against the conformational epitops of the protein. So my thought is trying to combine
denaturing protein (after cleaning and dialysis) with small quantity of the protein in native form
(purified under native conditions).
I would like to get ur feedback please.
should work the way you hope.
one more thing u shud be keeping in mind is, how IMMUNOGENIC ur proteins is? which determines the strength (affinity) of the product (Ab).
otherwise u r on the way to get good antibodies!!!!