Plant Protein Extraction - (Mar/29/2007 )
I want my students to extract plant proteins (from cabbage, beans or peas) for a lab exercise. I would like to use a kit for this. Does anyone know of a good plant protein extraction kit?
[Rather than using a Kit you can use Tris HCL buffer pH-6.8 or 8.0 to extract proteins by squashing the fresh samples in chilled buffer and fiter through 8 layerd cheese muslin cloth . centrifuge for 14,000rpm for 20min at 4 dgrees. collect the supernatant and go for a amminum sulphate precipitaion with satjrated amminun sulp[hate solution. overnight at 4 dgrees at constant sterr. then estimate the amount of protein obtained by Stabderd porotein estimation by bradford, lowery, BCA assay and run minimmun 5-10 ug of sample on SDS-PAGE for confermation and stain with Commasies blue.
hi! after extracting with tris-hcl buffer, would 4C or 8C be alright for long-term storage (months)? i dont want to store at -80 because im afraid the proteins might crystallize. thanks!