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BCA assay - folded or denatured protein (Jul/15/2005 )

Hi all

I run a BCA assay (bicinchoninic acid assay) to determine the concentration of protein in cell extracts.

Will this assay still work with denatured protein that has been heated in laemmli buffer.

i.e. does protein still reduce Cu(II) to Cu(I) regardless of whether it is folded or denatured


Thanks in advance


since peptide bonds and AA-residues are still present BCA will be ok

suggestion: in this case reaction should be easier; is the signal enhanced?


assuming BCA liks to peptidic bond it would be ok.