what generally methods to improve the protein solubility - (Apr/18/2005 )

as the title
thanks

is your question regarding enhancing solublity during protein expression system? if yes I have found this website to be very helpful http://www.embl.de/ExternalInfo/protein_un...owchart_ext.htm

or is it a solubility from a membrane fraction or a total cell lysate? please find enclosed a flow chart that I have created "Protein Analysis from Sample Preparation to Identification"
Good Luck!