Protein Overexpression - Inquiring about growth time before induction (Dec/08/2008 )
I have been following a protocol for overexpressing my protein but I have been getting very low yield. So instead of growing the cells until they reach an OD of 0.5-0.6 as indicated on the protocol, I have been growing them until they have an OD of ~1.7. The yield has significantly increased. Should I be worried about letting the cells grow that long before induction with IPTG (~4:30hrs)? Basically, I would like to know how long is too long when growing cells for protein overexpression.
Thank you very much for your time and help.
If it works stick with it!! I guess just don't grow them so much that they run out of food and room and start to die en mass before you induce.