protein solubilization with NDSB - (May/03/2001 )
I was wondering if anyone has tried solubilising an insoluble protein with a NDSB. I am going to try it, but many people around my lab are sceptical about its success. I would like to hear any stories from anyone who has tried them.
I have had great success with NDSB. I overexpress protein in baculovirus and had great difficulty with solubility. NDSB 256 increased my yield approx 60-80% of resolubilization with sarkosyl alone.
I have recently found (the hard way!) that NDSB from commerical sources must be further purified through a mixed bed ion exchange resin like TMD-8, before use in refolding
Otherwise, it still works great for my nuclear DNA binding protein.