DMSO for protein stability at -80oC - protein storage (Aug/07/2008 )
Hello. My problem is on protein storage. My purified protein precipitates after storage at -80oC. Someone suggested to add DMSO to a final concentration of 20%, but I've read somewhere to add glycerol, 25 to 50%. I would really appreciate if someone can help me with my problem. Thank you very much.
-tenuissimus-
QUOTE (tenuissimus @ Aug 7 2008, 08:14 PM)
Hello. My problem is on protein storage. My purified protein precipitates after storage at -80oC. Someone suggested to add DMSO to a final concentration of 20%, but I've read somewhere to add glycerol, 25 to 50%. I would really appreciate if someone can help me with my problem. Thank you very much.
I would use glycerol; to stabilize a protein, some ionic strength or trace elements may be necessary...
-The Bearer-
I have also used Glycerol for storage purposes.
-labrat612-
QUOTE (The Bearer @ Aug 8 2008, 07:14 AM)
QUOTE (tenuissimus @ Aug 7 2008, 08:14 PM)
Hello. My problem is on protein storage. My purified protein precipitates after storage at -80oC. Someone suggested to add DMSO to a final concentration of 20%, but I've read somewhere to add glycerol, 25 to 50%. I would really appreciate if someone can help me with my problem. Thank you very much.
I would use glycerol; to stabilize a protein, some ionic strength or trace elements may be necessary...
thanks!
-tenuissimus-
QUOTE (labrat612 @ Aug 8 2008, 08:51 AM)
I have also used Glycerol for storage purposes.
thanks!
-tenuissimus-