How to calculate protein size in Kilodaltons - (Apr/16/2006 )
I want to know the formula for determining size of proteins in kilodlaton and how we can determine that what percentage of sds gel is required to isolate protein according to their size.
For example I have 2000 bp gene fargemet how can I detemine size of proteins in Kilodaltons and what percentage of proteins is required for separation according to their size.
best regards
a very, very rough approximation can be made by dividing the number of base pairs (or bases, if single stranded) by 3 for the number of codons. multiply the number of codons by 100 (a very rough approximation of the average weight of an amino acid). the result is a rough approximation of the formula weight of your protein in daltons.
did i say "rough" enough?
Bu how much base pair or amino acid sequence is equal to 1 kilodalton or daltons.
did i say "rough" enough?
as mdfenko said, small correction needs to be done ofcourse it does not make big difference,
that is
1 amino acid = 110 Dalton
the answer for ur question is in here
1kilodalton = 1000 daltons
(since 110 dalton = 1 amino acid weight approximately)
1000 daltons = 9.09 or 10 amino acids
(since each amino acid corresponds to 3 bases)
10amino acids X 3 bases = 30 bases
(since each nucleotide weighs approximately 330 daltons)
30 bases(not pairs) X 330 dalton = 9900 Da
inconclusion, 10 amino acids corresponds to 1KDa of protein
OR
30 bases corresponds to 10KDa of DNA
i hope u understand
gud luk
sravan k. payeli
YES YES I WANT TO KNOW SOMETHNG LIKE THAT. ITS AMAZING. I LIKE THAT. I GOT CONCEPT.
10 AMINO ACID IS EQUAL TO 10 KILDALTONS OF PROTEINS. IS IT????.
I THINK U DID SOME TYPING MISTAKE BECAUSE U WROTE
10 amino acids corresponds to 1KDa of protein
OR
30 bases corresponds to 10KDa of DNA
as mdfenko said, small correction needs to be done ofcourse it does not make big difference,
that is
1 amino acid = 110 Dalton
the answer for ur question is in here
1kilodalton = 1000 daltons
(since 110 dalton = 1 amino acid weight approximately)
1000 daltons = 9.09 or 10 amino acids
(since each amino acid corresponds to 3 bases)
10amino acids X 3 bases = 30 bases
(since each nucleotide weighs approximately 330 daltons)
30 bases(not pairs) X 330 dalton = 9900 Da
inconclusion, 10 amino acids corresponds to 1KDa of protein
OR
30 bases corresponds to 10KDa of DNA
i hope u understand
gud luk
sravan k. payeli
No, payeli is correct - almost.
10 amino acids weighs about 1 kilodalton whereas the weight of the piece of DNA needed to code those 10 amino acids is actually ~20 kilodaltons (30 bp - as the DNA is double stranded initially).
hi samita !
mdfenko gave the shortest possible answer i suppose . the mol. wt of the protein coded by a 2000 bp gene will be
2000bp gene = 666.67 codons app. = 666.67 amino acids app.
1 amino acid = 110 daltons
666.67 amino acids = 110 x 666.67 =73333.7 daltons or 73.337 ~ 73.3 kDa.
you can separate this protein in a 10% PAGE