protein refolding from IB.. why done at alkaline pH? - (May/01/2008 )
hii.. why is protein refolding from inclusion bodies.. done at alkaline pH? my protein has a pI of 6.. refolding procedures say the refolding is done in tris buffer,pH 9.. with arginine cysteine cystine.. could someone tell me why this is chosen?
-alice!-
QUOTE (alice! @ May 1 2008, 06:33 AM)
hii.. why is protein refolding from inclusion bodies.. done at alkaline pH? my protein has a pI of 6.. refolding procedures say the refolding is done in tris buffer,pH 9.. with arginine cysteine cystine.. could someone tell me why this is chosen?
basic pH is in favour of disulfide bond formation.
-genehunter-1-